| Host: |
Rabbit |
| Applications: |
IHC/WB |
| Reactivity: |
Human |
| Note: |
STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS. |
| Short Description: |
Rabbit polyclonal antibody anti-ABCA4 (1450-1500 aa) is suitable for use in Immunohistochemistry and Western Blot research applications. |
| Clonality: |
Polyclonal |
| Conjugation: |
Unconjugated |
| Isotype: |
IgG |
| Formulation: |
Lyophilised |
| Purification: |
IgG purified |
| Dilution Range: |
IHC, WB. A dilution of 1:300 to 1:2000 is recommended. The optimal dilution should be determined by the end user. Not yet tested in other applications. |
| Storage Instruction: |
Maintain the lyophilised/reconstituted antibodies frozen at-20°C for long term storage and refrigerated at 2-8°C for a shorter term. When reconstituting, Glycerol (1:1) may be added for an additional stability. Avoid freeze and thaw cycles. |
| Gene Symbol: |
ABCA4 |
| Gene ID: |
24 |
| Uniprot ID: |
ABCA4_HUMAN |
| Immunogen Region: |
1450-1500 aa |
| Specificity: |
Specific for ABCA4. |
| Immunogen: |
A synthetic peptide from aa region 1450-1500 of human ABCA4 conjugated to blue carrier protein was used as the antigen. |
| Post Translational Modifications | Proteolytic cleavage by trypsin leads to a 120-kDa N-terminal fragment and a 115-kDa C-terminal fragment that are linked through disulfide bonds. N-glycosylated. Phosphorylation is independent of light exposure and modulates ATPase activity. |
| Function | Flippase that catalyzes in an ATP-dependent manner the transport of retinal-phosphatidylethanolamine conjugates like the 11-cis and all-trans isomers of N-retinylidene-phosphatidylethanolamine from the lumen to the cytoplasmic leaflet of photoreceptor outer segment disk membranes, where N-cis-retinylidene-phosphatidylethanolamine (N-cis-R-PE) is then isomerized to its all-trans isomer (N-trans-R-PE) and reduced by RDH8 to produce all-trans-retinol (all-trans-rol) and therefore prevents the accumulation of excess of 11-cis-retinal and its schiff-base conjugate and the formation of toxic bisretinoid. May display both ATPase and GTPase activity that is strongly influenced by the lipid environment and the presence of retinoid compounds. Binds the unprotonated form of N-retinylidene-phosphatidylethanolamine with high affinity in the absence of ATP, and ATP binding and hydrolysis induce a protein conformational change that causes the dissociation of N-retinylidene-phosphatidylethanolamine. |
| Protein Name | Retinal-Specific Phospholipid-Transporting Atpase Abca4Atp-Binding Cassette Sub-Family A Member 4Rim Abc TransporterRim ProteinvRmpRetinal-Specific Atp-Binding Cassette TransporterStargardt Disease Protein |
| Database Links | Reactome: R-HSA-2453864Reactome: R-HSA-2453902Reactome: R-HSA-382556 |
| Cellular Localisation | MembraneMulti-Pass Membrane ProteinEndoplasmic ReticulumCytoplasmic VesicleCell ProjectionCiliumPhotoreceptor Outer SegmentLocalized To The Rim And Incisures Of Rod Outer Segments Disks |
| Alternative Antibody Names | Anti-Retinal-Specific Phospholipid-Transporting Atpase Abca4 antibodyAnti-Atp-Binding Cassette Sub-Family A Member 4 antibodyAnti-Rim Abc Transporter antibodyAnti-Rim Proteinv antibodyAnti-Rmp antibodyAnti-Retinal-Specific Atp-Binding Cassette Transporter antibodyAnti-Stargardt Disease Protein antibodyAnti-ABCA4 antibodyAnti-ABCR antibody |
Information sourced from Uniprot.org
12 months for antibodies. 6 months for ELISA Kits. Please see website T&Cs for further guidance
