Anti-ABCA2 antibody (140-350) (STJ119146)
SPECIFICATIONS
ClonalityPolyclonal
HostRabbit
ConjugationUnconjugated
IsotypeIgG
General Information
| Short Description | Rabbit polyclonal ABCA2 (140-350) antibody for use in WB, IF, ICC and ELISA in human, mouse and rat samples. Datasheet included with dilution recommendations, and related reagents. |
| Applications | WB/IF/ICC/ELISA |
| Host | Rabbit |
| Reactivity | Human/Mouse/Rat |
| Note | STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS. |
Product Properties
| Clonality | Polyclonal |
| Isotype | IgG |
| Conjugation | Unconjugated |
| Concentration | Lot specific |
| Purification | Affinity purification |
| Dilution Range | WB:1:500-1:1000IF/ICC:1:50-1:200ELISA:Recommended starting concentration is 1 Mu g/mL. Please optimize the concentration based on your specific assay requirements. |
| Formulation | PBS with 0.01% Thimerosal, 50% Glycerol, pH 7.3. |
| Storage Instruction | Store at-20ยฐC for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles. |
Target Information
| Gene Symbol | ABCA2 |
| Gene ID | 20 |
| Uniprot ID | ABCA2_HUMAN |
| Immunogen Region | 140-350 |
| Immunogen Sequence | SHLDRSTVSSFSLDSVARNP QELWRFLTQNLSLPNSTAQA LLAARVDPPEVYHLLFGPSS ALDSQSGLHKGQEPWSRLGG NPLFRMEELLLAPALLEQLT CTPGSGELGRILTVPESQKG ALQGYRDAVCSGQAAARARR FSGLSAELRNQLDVAKVSQQ LGLDAPNGSDSSPQAPPPRR LQALLGDLLDAQKVLQDVDV LSALALLLPQG |
| Specificity | Recombinant fusion protein containing a sequence corresponding to amino acids 140-350 of human ABCA2 (NP_001597.2). |
Additional Info
| Tissue Specificity | Isoform 3: Highly expressed in the brain,peripheral blood leukocytes and ovary, whereas lower levels of expression is observed in kidney and liver. Isoform 4: Weakly expressed in brain and highly in peripheral blood leukocytes. |
| Post Translational Modifications | Methylated at Gln-271 by N6AMT1. |
| Function | Probable lipid transporter that modulates cholesterol sequestration in the late endosome/lysosome by regulating the intracellular sphingolipid metabolism, in turn participates in cholesterol homeostasis (Probable). May alter the transbilayer distribution of ceramide in the intraluminal membrane lipid bilayer, favoring its retention in the outer leaflet that results in increased acid ceramidase activity in the late endosome/lysosome, facilitating ceramide deacylation to sphingosine leading to the sequestration of free cholesterol in lysosomes. In addition regulates amyloid-beta production either by activating a signaling pathway that regulates amyloid precursor protein transcription through the modulation of sphingolipid metabolism or through its role in gamma-secretase processing of APP. May play a role in myelin formation. |
| Protein Name | Atp-Binding Cassette Sub-Family A Member 2Atp-Binding Cassette Transporter 2Atp-Binding Cassette 2 |
| Database Links | Reactome: R-HSA-1369062 |
| Cellular Localisation | Endosome MembraneMulti-Pass Membrane ProteinLysosome MembraneForms DiscretePunctate Intracellular Vesicles |
| Alternative Antibody Names | Anti-Atp-Binding Cassette Sub-Family A Member 2 antibodyAnti-Atp-Binding Cassette Transporter 2 antibodyAnti-Atp-Binding Cassette 2 antibodyAnti-ABCA2 antibodyAnti-ABC2 antibodyAnti-KIAA1062 antibody |
Information sourced from Uniprot.org