| Host: |
Rabbit |
| Applications: |
IHC/WB |
| Reactivity: |
Human |
| Note: |
STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS. |
| Short Description: |
Rabbit polyclonal antibody anti-ABCA12 (1450-1500 aa) is suitable for use in Immunohistochemistry and Western Blot research applications. |
| Clonality: |
Polyclonal |
| Conjugation: |
Unconjugated |
| Isotype: |
IgG |
| Formulation: |
Lyophilised |
| Purification: |
IgG purified |
| Dilution Range: |
IHC, WB. A dilution of 1:300 to 1:2000 is recommended. The optimal dilution should be determined by the end user. Not yet tested in other applications. |
| Storage Instruction: |
Maintain the lyophilised/reconstituted antibodies frozen at-20°C for long term storage and refrigerated at 2-8°C for a shorter term. When reconstituting, Glycerol (1:1) may be added for an additional stability. Avoid freeze and thaw cycles. |
| Gene Symbol: |
ABCA12 |
| Gene ID: |
26154 |
| Uniprot ID: |
ABCAC_HUMAN |
| Immunogen Region: |
1450-1500 aa |
| Specificity: |
Specific for ABCA12. |
| Immunogen: |
A synthetic peptide from aa region 1450-1500 of human ABCA12 conjugated to an immunogenic carrier protein was used as the antigen. |
| Function | Transports lipids such as glucosylceramides from the outer to the inner leaflet of lamellar granules (LGs) membrane, whereby the lipids are finally transported to the keratinocyte periphery via the trans-Golgi network and LGs and released to the apical surface of the granular keratinocytes to form lipid lamellae in the stratum corneum of the epidermis, which is essential for skin barrier function. In the meantime, participates in the transport of the lamellar granules-associated proteolytic enzymes, in turn regulates desquamation and keratinocyte differentiation. Furthermore, is essential for the regulation of cellular cholesterol homeostasis by regulating ABCA1-dependent cholesterol efflux from macrophages through interaction with NR1H2 and ABCA1. Plays pleiotropic roles in regulating glucose stimulated insulin secretion from beta cells, regulating the morphology and fusion of insulin granules, lipid raft abundance and the actin cytoskeleton. Also involved in lung surfactant biogenesis. |
| Protein Name | Glucosylceramide Transporter Abca12Atp-Binding Cassette Sub-Family A Member 12Atp-Binding Cassette Transporter 12Atp-Binding Cassette 12 |
| Database Links | Reactome: R-HSA-1369062Reactome: R-HSA-5682294 |
| Cellular Localisation | Cytoplasmic VesicleSecretory Vesicle MembraneMulti-Pass Membrane ProteinGolgi Apparatus MembraneLocalizes In The Limiting Membrane Of The Lamellar Granules (Lgs)Trafficks From The Golgi Apparatus To The Lamellar Granules (Lgs) At The Cell Periphery In The Uppermost Granular Layer Keratinocytes Where Abca12-Positive Lgs Fuse With The Keratinocyte-Cell Membrane To Secrete Their Lipid Content To The Extracellular Space Of The Stratum CorneumCo-Localizes Through The Golgi Apparatus To The Cell Periphery With Glucosylceramide |
| Alternative Antibody Names | Anti-Glucosylceramide Transporter Abca12 antibodyAnti-Atp-Binding Cassette Sub-Family A Member 12 antibodyAnti-Atp-Binding Cassette Transporter 12 antibodyAnti-Atp-Binding Cassette 12 antibodyAnti-ABCA12 antibodyAnti-ABC12 antibody |
Information sourced from Uniprot.org
12 months for antibodies. 6 months for ELISA Kits. Please see website T&Cs for further guidance
