• Western blot analysis of recombinant SARS-COV-2 N Protein, using SARS-CoV-2 N Protein Rabbit mAb, BSA and glycerol free (STJ11101210) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 30 ng/10 ng/5 ng/2 ng per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 5s.

Anti-2019-nCoV antibody [S0MR] (STJ11101210)

SKU:
STJ11101210

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Host: Rabbit
Applications: WB/DB/IF/ICC/ELISA
Reactivity: SARS-CoV-2
Note: STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS.
Clonality: Monoclonal
Clone ID: S0MR
Conjugation: Unconjugated
Isotype: IgG
Formulation: PBS with 0.05% Proclin300, pH 7.3.
Purification: Affinity purification
Concentration: Lot specific
Dilution Range: WB:1:1000-1:6000
DB:1:500-1:2000
IF/ICC:1:200-1:800
ELISA:Recommended starting concentration is 1 Mu g/mL. Please optimize the concentration based on your specific assay requirements.
Storage Instruction: Store at 4°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles.
Specificity: Recombinant fusion protein of SARS-CoV-2 Nucleoprotein Full length ( P59595 ).
Immunogen Sequence: MSDNGPQSNQRSAPRITFGG PTDSTDNNQNGGRNGARPKQ RRPQGLPNNTASWFTALTQH GKEELRFPRGQGVPINTNSG PDDQIGYYRRATRRVRGGDG KMKELSPRWYFYYLGTGPEA SLPYGANKEGIVWVATEGAL NTPKDHIGTRNPNNNAATVL QLPQGTTLPKGFYAEGSRGG SQASSRSSSRSRGNSRNSTP GSSRGNSPARMASGGGETAL ALLLLDRLNQLESKVSGKG
Background Packages the positive strand viral genome RNA into a helical ribonucleocapsid (RNP and plays a fundamental role during virion assembly through its interactions with the viral genome and membrane protein M. Plays an important role in enhancing the efficiency of subgenomic viral RNA transcription as well as viral replication. May modulate transforming growth factor-beta signaling by binding host SMAD3.

Information sourced from Uniprot.org

12 months for antibodies. 6 months for ELISA Kits. Please see website T&Cs for further guidance