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Anti-14-3-3 sigma/Stratifin antibody (Internal) (STJ70838)

STJ70838 (0. 03µg/ml) staining of Human Skin lysate (35µg protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.
STJ70838 (0. 03µg/ml) staining of A431 (A) and U2OS (B) cell lysate (35µg protein in RIPA buffer). Detected by chemiluminescence.
STJ70838 (0. 03µg/ml) staining of Mouse (A) and Rat (B) Skin lysate (35µg protein in RIPA buffer). Detected by chemiluminescence.
STJ70838 (2µg/ml) staining of paraffin embedded Human Skin. Steamed antigen retrieval with Tris/EDTA buffer pH 9, HRP-staining.

Anti-14-3-3 sigma/Stratifin antibody (Internal) (STJ70838)

SKU:

STJ70838-100

£303.50

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General Information
Product Properties
Target Information
Additional Info
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Host:	Goat
Applications:	Pep-ELISA/WB/IHC
Reactivity:	Human/Mouse/Rat/Dog/Pig
Note:	STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS.
Short Description:	Goat polyclonal antibody anti-14-3-3 sigma/Stratifin (Internal) is suitable for use in ELISA, Western Blot and Immunohistochemistry research applications.

Clonality:	Polyclonal
Conjugation:	Unconjugated
Isotype:	IgG
Formulation:	0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin. NA
Purification:	Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Concentration:	0.5 mg/mL
Dilution Range:	WB-0.03-0.1µg/ml IHC-Recommended concentration, 2-4µg/ml ELISA-antibody detection limit dilution 1:16000.
Storage Instruction:	Store at-20°C on receipt and minimise freeze-thaw cycles.

Gene Symbol:	SFN
Gene ID:	2810
Uniprot ID:	1433S_HUMAN
Immunogen Region:	Internal
Accession Number:	NP_006133.1
Immunogen Sequence:	DLHTLSEDSYKDST

Post Translational Modifications	Ubiquitinated. Ubiquitination by RFFL induces proteasomal degradation and indirectly regulates p53/TP53 activation.
Function	Adapter protein implicated in the regulation of a large spectrum of both general and specialized signaling pathways. Binds to a large number of partners, usually by recognition of a phosphoserine or phosphothreonine motif. Binding generally results in the modulation of the activity of the binding partner. When bound to KRT17, regulates protein synthesis and epithelial cell growth by stimulating Akt/mTOR pathway. May also regulate MDM2 autoubiquitination and degradation and thereby activate p53/TP53. p53-regulated inhibitor of G2/M progression.
Protein Name	14-3-3 Protein Sigma Epithelial Cell Marker Protein 1 Stratifin
Database Links	Reactome: R-HSA-111447 Reactome: R-HSA-1445148 Reactome: R-HSA-5625740 Reactome: R-HSA-5628897 Reactome: R-HSA-6804114 Reactome: R-HSA-75035 Reactome: R-HSA-9614399 Reactome: R-HSA-9735871 Reactome: R-HSA-9755779
Cellular Localisation	Cytoplasm Nucleus Secreted May Be Secreted By A Non-Classical Secretory Pathway
Alternative Antibody Names	Anti-14-3-3 Protein Sigma antibody Anti-Epithelial Cell Marker Protein 1 antibody Anti-Stratifin antibody Anti-SFN antibody Anti-HME1 antibody

Information sourced from Uniprot.org

12 months for antibodies. 6 months for ELISA Kits. Please see website T&Cs for further guidance

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