| Host: | Mouse |
| Applications: | ELISA/WB/ICC/IF |
| Reactivity: | Human |
| Note: | STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS. |
| Short Description : | Mouse monoclonal anti-14-3-3 epsilon (1-255aa) for use in ELISA, WB, ICC and IF in Human samples. Datasheet included with dilution recommendations, and related reagents. |
| Clonality : | Monoclonal |
| Clone ID : | AT4F8 |
| Conjugation: | Unconjugated |
| Isotype: | IgG2bk |
| Formulation: | Liquid in phosphate-Buffered Saline (pH 7.4) with 0.02% Sodium Azide, 10% Glycerol |
| Purification: | By protein-A affinity chromatography |
| Concentration: | 1 mg/mL |
| Storage Instruction: | For short term storage, keep at +2C to +8C for up to 1 week. For long term storage, aliquot and store at-20C, and avoid repeat freeze-thaw cycles. |
| Gene Symbol: | YWHAE |
| Gene ID: | 7531 |
| Uniprot ID: | 1433E_HUMAN |
| Immunogen Region: | 1-255aa |
| Accession Number: | NP_006752 |
| Immunogen: | Recombinant human14-3-3 epsilon (1-255aa) purified from E. coli |
| Post Translational Modifications | (Microbial infection) Cleaved by poliovirus protease 3C, leading to disruption of the interaction with RIGI. |
| Function | Adapter protein implicated in the regulation of a large spectrum of both general and specialized signaling pathways. Binds to a large number of partners, usually by recognition of a phosphoserine or phosphothreonine motif. Binding generally results in the modulation of the activity of the binding partner. Positively regulates phosphorylated protein HSF1 nuclear export to the cytoplasm. Plays a positive role in the antiviral signaling pathway upstream of TBK1 via interaction with RIGI. Mechanistically, directs RIGI redistribution from the cytosol to mitochondrial associated membranes where it mediates MAVS-dependent innate immune signaling during viral infection. Plays a role in proliferation inhibition and cell cycle arrest by exporting HNRNPC from the nucleus to the cytoplasm to be degraded by ubiquitination. |
| Protein Name | 14-3-3 Protein Epsilon14-3-3e |
| Database Links | Reactome: R-HSA-111447Reactome: R-HSA-1445148Reactome: R-HSA-2028269Reactome: R-HSA-205025Reactome: R-HSA-2565942Reactome: R-HSA-3371453Reactome: R-HSA-3371511Reactome: R-HSA-380259Reactome: R-HSA-380270Reactome: R-HSA-380284Reactome: R-HSA-380320Reactome: R-HSA-5620912Reactome: R-HSA-5625740Reactome: R-HSA-5628897Reactome: R-HSA-75035Reactome: R-HSA-8854518Reactome: R-HSA-8862803Reactome: R-HSA-8876198Reactome: R-HSA-9735871Reactome: R-HSA-9755779Reactome: R-HSA-9856649 |
| Cellular Localisation | NucleusCytoplasmMelanosomeIdentified By Mass Spectrometry In Melanosome Fractions From Stage I To Stage Iv |
| Alternative Antibody Names | Anti-14-3-3 Protein Epsilon antibodyAnti-14-3-3e antibodyAnti-YWHAE antibody |
Information sourced from Uniprot.org
