| Applications: |
WB |
| Note: |
STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS. |
| Short Description: |
ALDH1A1 Positive Control for STJ500065 is synthetically produced from the sequence and is suitable for use in western blot applications. |
| Formulation: |
Provided as 100 uL ready-to-use, in SDS-PAGE sample buffer (Laemelli's buffer) containing Tris, pH 6.8, 1 % SDS, Glycerol and Bromophenolblue blue as tracking dye. The sample is reduced by adding 2% beta mercaptoethanol. The protein concentration is |
| Dilution Range: |
WB: 1:2, 500-1:5, 000 |
| Storage Instruction: |
Store at-20°C for long term storage. Avoid freeze-thaw cycles. |
| Gene Symbol: |
Aldh1a1 |
| Gene ID: |
24188 |
| Uniprot ID: |
AL1A1_RAT |
| Specificity: |
This is positive control is recommended for use in combination with ALDH1A1 antibody STJ500065. |
| Tissue Specificity | Strongly expressed in kidney, lung, testis, intestine, stomach, and trachea, but weakly in the liver. |
| Post Translational Modifications | The N-terminus is blocked most probably by acetylation. |
| Function | Cytosolic dehydrogenase that catalyzes the irreversible oxidation of a wide range of aldehydes to their corresponding carboxylic acid. Functions downstream of retinol dehydrogenases and catalyzes the oxidation of retinaldehyde into retinoic acid, the second step in the oxidation of retinol/vitamin A into retinoic acid. This pathway is crucial to control the levels of retinol and retinoic acid, two important molecules which excess can be teratogenic and cytotoxic. Also oxidizes aldehydes resulting from lipid peroxidation like (E)-4-hydroxynon-2-enal/HNE, malonaldehyde and hexanal that form protein adducts and are highly cytotoxic. By participating for instance to the clearance of (E)-4-hydroxynon-2-enal/HNE in the lens epithelium prevents the formation of HNE-protein adducts and lens opacification. Functions also downstream of fructosamine-3-kinase in the fructosamine degradation pathway by catalyzing the oxidation of 3-deoxyglucosone, the carbohydrate product of fructosamine 3-phosphate decomposition, which is itself a potent glycating agent that may react with lysine and arginine side-chains of proteins. Has also an aminobutyraldehyde dehydrogenase activity and is probably part of an alternative pathway for the biosynthesis of GABA/4-aminobutanoate in midbrain, thereby playing a role in GABAergic synaptic transmission. |
| Peptide Name | Aldehyde Dehydrogenase 1a1 3-Deoxyglucosone DehydrogenaseAldh-E1AlhdiiAldehyde Dehydrogenase Family 1 Member A1Aldehyde Dehydrogenase - CytosolicRetinal Dehydrogenase 1Raldh 1Raldh1 |
| Database Links | Reactome: R-RNO-5365859Reactome: -RNO-70350Reactome: -RNO-71384 |
| Cellular Localisation | CytoplasmCytosolCell ProjectionAxon |
| Alternative Peptide Names | Aldehyde Dehydrogenase 1a1 protein3-Deoxyglucosone Dehydrogenase proteinAldh-E1 proteinAlhdii proteinAldehyde Dehydrogenase Family 1 Member A1 proteinAldehyde Dehydrogenase - Cytosolic proteinRetinal Dehydrogenase 1 proteinRaldh 1 proteinRaldh1 proteinAldh1a1 proteinAldh protein |
Information sourced from Uniprot.org
12 months for antibodies. 6 months for ELISA Kits. Please see website T&Cs for further guidance
