| Host: |
CHO cells |
| Note: |
STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS. |
| Short Description: |
2B4-protein was developed from cho cells. For use in research applications. |
| Formulation: |
PBS pH 6.0 |
| Purification: |
Purified by Ni-affinity chromatography, ion-exchange chromatography and desalting chromatography from serum-free CHO growth media |
| Storage Instruction: |
Store at-70°C upon receipt. Recommended to aliquot into smaller quantities. Avoid repeated freeze-thaw cycles |
| Tissue Specificity | Expressed in spleen, PBL, followed by lung, liver, testis and small intestine. Expressed in all natural killer (NK) cells, monocytes and basophils, TCR-gamma/delta+ T-cells, monocytes, basophils, and on a subset of CD8(+) T-cells. |
| Post Translational Modifications | N-linked glycosylation is essential for the binding to its ligand CD48. Also O-glycosylated, in contrast, O-linked sialylation has a negative impact on ligand binding. Phosphorylated by FYN and CSK on tyrosine residues following activation. Coligation with inhibitory receptors such as KIR2DL1 inhibits phosphorylation upon contact of NK cells with sensitive target cells. |
| Function | Heterophilic receptor of the signaling lymphocytic activation molecule (SLAM) family.its ligand is CD48. SLAM receptors triggered by homo- or heterotypic cell-cell interactions are modulating the activation and differentiation of a wide variety of immune cells and thus are involved in the regulation and interconnection of both innate and adaptive immune response. Activities are controlled by presence or absence of small cytoplasmic adapter proteins, SH2D1A/SAP and/or SH2D1B/EAT-2. Acts as activating natural killer (NK) cell receptor. Activating function implicates association with SH2D1A and FYN. Downstreaming signaling involves predominantly VAV1, and, to a lesser degree, INPP5D/SHIP1 and CBL. Signal attenuation in the absence of SH2D1A is proposed to be dependent on INPP5D and to a lesser extent PTPN6/SHP-1 and PTPN11/SHP-2. Stimulates NK cell cytotoxicity, production of IFN-gamma and granule exocytosis. Optimal expansion and activation of NK cells seems to be dependent on the engagement of CD244 with CD48 expressed on neighboring NK cells. Acts as costimulator in NK activation by enhancing signals by other NK receptors such as NCR3 and NCR1. At early stages of NK cell differentiation may function as an inhibitory receptor possibly ensuring the self-tolerance of developing NK cells. Involved in the regulation of CD8(+) T-cell proliferation.expression on activated T-cells and binding to CD48 provides costimulatory-like function for neighboring T-cells. Inhibits inflammatory responses in dendritic cells (DCs). |
| Protein Name | Natural Killer Cell Receptor 2b4Nk Cell Activation-Inducing LigandNailNk Cell Type I Receptor Protein 2b4Nkr2b4H2b4Slam Family Member 4Slamf4Signaling Lymphocytic Activation Molecule 4Cd Antigen Cd244 |
| Database Links | Reactome: R-HSA-202733 |
| Cellular Localisation | MembraneSingle-Pass Type I Membrane ProteinCell MembraneReceptor Engagement Results In A Recruitment To Lipid Drafts Essential For The Subsequent Tyrosine Phosphorylation Of The Itsms |
| Alternative Protein Names | Natural Killer Cell Receptor 2b4 proteinNk Cell Activation-Inducing Ligand proteinNail proteinNk Cell Type I Receptor Protein 2b4 proteinNkr2b4 proteinH2b4 proteinSlam Family Member 4 proteinSlamf4 proteinSignaling Lymphocytic Activation Molecule 4 proteinCd Antigen Cd244 proteinCD244 protein2B4 protein |
Information sourced from Uniprot.org
12 months for antibodies. 6 months for ELISA Kits. Please see website T&Cs for further guidance
