Signal Transduction | Research Area | St John's Laboratory

Western blot analysis of HepG2, using PerilipinA antibody (STJ118726) at 1:400 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 90s.

Anti-PLIN1 antibody (400-500) (STJ118726)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/ELISA

Reactivity :

Human

£112.50 - £524.50

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Confocal imaging of paraffin-embedded Rat brown fat tissue using Perilipin A Rabbit monoclonal antibody (STJ11102149, dilution 1:100) followed by a further incubation with Cy3 Goat Anti-Rabbit IgG (H+L) ( dilution 1:500) (Red). DAPI was used for nuclear staining (Blue). Objective: 40x. Perform high pressure antigen retrieval with 0. 01 M citrate buffer (pH 6. 0) prior to IF staining.

Anti-PLIN1 antibody (400-500) [S9MR] (STJ11102149)

Clonality :

Monoclonal

Host :

Rabbit

Applications :

WB/IHC-P/IF/ICC/ELISA

Reactivity :

Mouse/Rat

£146.50 - £642.50

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Western blot analysis of Perilipin A in Human fetal liver lysates using Perilipin 1 antibody.

Anti-PLIN1 antibody (475-500 aa) [R08-9B4] (STJA0014403)

Clonality :

Monoclonal

Host :

Rabbit

Applications :

WB/ICC/IF

Reactivity :

Human/Mouse

£264.50

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Anti-PLIN1 antibody (STJA0009775)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB

Reactivity :

Human/Mouse/Rat

£48.00 - £252.00

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Western blot analysis of various lysates using PLIN3 Rabbit polyclonal antibody (STJ116409) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Enhanced Kit. Exposure time: 30s.

Anti-PLIN3 antibody (135-434) (STJ116409)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/ELISA

Reactivity :

Human

£112.50 - £524.50

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Western blot analysis of various lysates using PLIN3 Rabbit polyclonal antibody (STJ118221) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 90s.

Anti-PLIN3 antibody (135-434) (STJ118221)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/ELISA

Reactivity :

Human/Mouse

£112.50 - £524.50

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Western blot analysis of lysates from K562 cells, primary antibody was diluted at 1:1000, 4°C over night

Anti-PLIN3 antibody (STJ193144)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/ELISA

Reactivity :

Human/Rat/Mouse

£48.00 - £252.00

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Immunofluorescence analysis of A549 cells, using PLK1 Antibody. The picture on the right is blocked with the synthesized peptide.

Anti-PLK1 antibody (176-225 aa) (STJ95162)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/IHC/IF/ELISA

Reactivity :

Human/Mouse/Rat

£48.00 - £252.00

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Western blot analysis of extracts of various cell lines, using PLK1 antibody (STJ11104170) at1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (STJS000856) at1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 60s.

Anti-PLK1 antibody (272-495) [S4170RM] (STJ11104170)

Clonality :

Monoclonal

Host :

Rabbit

Applications :

WB/ELISA

Reactivity :

Human/Mouse/Rat/Monkey

£129.50 - £574.50

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Western blot analysis of lysates from Rat testis using PLK1 Rabbit monoclonal antibody (STJ11104884) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Enhanced Kit. Exposure time: 120s.

Anti-PLK1 antibody (272-495) [S4884RM] (STJ11104884)

Clonality :

Monoclonal

Host :

Rabbit

Applications :

WB/ELISA

Reactivity :

Human/Mouse/Rat

£129.50 - £574.50

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Immunocytochemistry analysis of PLK1 (green) in Hela using PLK1 antibody, and DAPI (blue)

Anti-PLK1 antibody (455-474 aa) [R07-3E0] (STJA0012682)

Clonality :

Monoclonal

Host :

Rabbit

Applications :

WB/ICC/IF/IP

Reactivity :

Human/Rat

£264.50

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Western blot analysis of extracts from NIH-3T3 cells, using Plk1 Polyclonal Antibody.. Secondary antibody was diluted at 1:20000

Anti-PLK1 antibody (80-160 aa) (STJ96419)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/IHC/IF/ELISA

Reactivity :

Human/Mouse/Rat

£48.00 - £252.00

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Anti-PLK1 antibody [3H10] (STJA0019789)

Clonality :

Monoclonal

Applications :

ELISA

Reactivity :

Human

£341.50 - £1,447.50

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Western blot analysis of 1) PC12 Cell, 2) HepG2 Cell lysate using PLK1 mouse monoclonal antibody diluted at 1:2,000.

Anti-PLK1 antibody [Mix] (STJ196690)

Clonality :

Monoclonal

Host :

Mouse

Applications :

IHC/WB

Reactivity :

Human/Mouse/Rat

£174.00 - £252.00

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Anti-PLPPR2 antibody (STJA0009526)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB

Reactivity :

Human/Mouse/Rat

£48.00 - £252.00

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Anti-PLPPR3 antibody (STJA0008993)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB

Reactivity :

Human/Mouse/Rat

£48.00 - £252.00

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Anti-PLPPR4 antibody (55-80 aa) (STJ192133)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/ELISA

Reactivity :

Human/Rat/Mouse

£48.00 - £252.00

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Anti-PLPPR5 antibody (STJA0004445)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/IHC/ELISA

Reactivity :

Human/Mouse

£140.50 - £415.50

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Western blot analysis of extracts of various cell lines, using Plexin A1 Rabbit monoclonal antibody (STJ11101931) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 10s.

Anti-PLXNA1 antibody (100-200) [S1MR] (STJ11101931)

Clonality :

Monoclonal

Host :

Rabbit

Applications :

WB/ELISA

Reactivity :

Human/Mouse/Rat

£129.50 - £574.50

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Anti-PLXNA1 antibody (Sema Domain) (STJA0005538)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/ICC/IHC/IP

Reactivity :

Human/Mouse/Rat

£367.50

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Anti-PLXNA1 antibody (Sema Domain) (STJA0005540)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/ICC

Reactivity :

Human/Mouse/Rat

£367.50

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Anti-PLXNA1 antibody (Sema Domain) [M535] (STJA0005539)

Clonality :

Monoclonal

Host :

Mouse

Applications :

WB/ICC/IHC/IP

Reactivity :

Human/Mouse/Rat

£394.50

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Anti-PLXNA1 antibody (STJA0009804)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB

Reactivity :

Human/Mouse

£48.00 - £252.00

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Anti-PLXNA1/Plexin-A1 antibody [SAA1781] (STJA0020209)

Clonality :

Monoclonal

Host :

Mouse

Applications :

ELISA

Reactivity :

Human

£341.50 - £1,447.50

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Anti-PLXNB1 antibody (90-170 aa) (STJ191172)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/ELISA

Reactivity :

Human/Mouse

£48.00 - £252.00

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Anti-PLXNB1 antibody (C-Term) (STJA0005541)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/ICC

Reactivity :

Human/Mouse/Rat

£367.50

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Anti-PLXNB1 antibody [SAA1502] (STJA0019930)

Clonality :

Monoclonal

Host :

Mouse

Applications :

ELISA

Reactivity :

Human

£341.50 - £1,447.50

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Identification of a Novel Angiogenesis Signalling circSCRG1/miR-1268b/NR4A1 Pathway in Atherosclerosis and the Regulatory Effects of TMP-PF In Vitro MOLECULES Keji Chen WB Human human umbilical vein endothelial cells (HUVECs)

Anti-PLXND1 antibody (170-250 aa) (STJ192394)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/ELISA

Reactivity :

Human/Mouse

£48.00 - £252.00

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Anti-PLXND1 antibody (C-Term) (STJA0005544)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB

Reactivity :

Human/Mouse/Rat

£367.50

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Anti-PLXND1 antibody (Cytoplasmic) (STJA0005542)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB

Reactivity :

Human/Mouse/Rat

£367.50

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Anti-PLXND1 antibody (Sema Domain) (STJA0005543)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB

Reactivity :

Human/Mouse/Rat

£367.50

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Immunohistochemical analysis of paraffin-embedded human-testis, antibody was diluted at 1:200

Anti-PMCH antibody (112-161 aa) (STJ98825)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

IHC/IF/ELISA

Reactivity :

Human/Mouse/Rat

£48.00 - £252.00

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Western blot analysis of extracts of various cell lines, using PMCH antibody (STJ28775) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 10s.

Anti-PMCH antibody (22-165) (STJ28775)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/ELISA

Reactivity :

Human/Mouse

£112.50 - £524.50

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Western blot analysis of various cells using PMEPA1 Polyclonal Antibody diluted at 1：500

Anti-PMEPA1 antibody (101-150 aa) (STJ95165)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/IHC/IF/ELISA

Reactivity :

Human/Rat/Mouse

£48.00 - £252.00

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Western blot analysis of various lysates using PMEPA1 Rabbit polyclonal antibody (STJ114064) at 1:3000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 30s.

Anti-PMEPA1 antibody (173-252) (STJ114064)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/ELISA

Reactivity :

Human/Mouse/Rat

£112.50 - £524.50

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Immunohistochemistry analysis of PMEPA1 in paraffin-embedded mouse lung using PMEPA1 Rabbit polyclonal antibody (STJ118982) at dilution of 1:100 (40x lens). Perform microwave antigen retrieval with 10 mM PBS buffer pH 7. 2 before commencing with immunohistochemistry staining protocol.

Anti-PMEPA1 antibody (173-252) (STJ118982)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/IHC-P/ELISA

Reactivity :

Human/Mouse/Rat

£112.50 - £524.50

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Western blot analysis of 823 22RV1 HELA HEPG2 Cell Lysate, antibody was diluted at 1:2000. Secondary antibody was diluted at 1:20000

Anti-PMEPA1 antibody (240-287 aa) (STJ98968)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/ELISA

Reactivity :

Human/Rat/Mouse

£48.00 - £252.00

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Western blot analysis of various lysates using PMF1 Rabbit monoclonal antibody (STJ11104199) at1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Enhanced Kit. Exposure time: 180s.

Anti-PMF1 antibody (1-205) [S4199RM] (STJ11104199)

Clonality :

Monoclonal

Host :

Rabbit

Applications :

WB/ELISA

Reactivity :

Human/Mouse/Rat

£129.50 - £574.50

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Anti-PMF1 antibody (STJA0009249)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB

Reactivity :

Human/Mouse

£48.00 - £252.00

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Western blot analysis of extracts of various cell lines, using PML antibody (STJ11101744) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Enhanced Kit. Exposure time: 3min.

Anti-PML antibody (1-100) [S4MR] (STJ11101744)

Clonality :

Monoclonal

Host :

Rabbit

Applications :

WB/ELISA

Reactivity :

Human/Mouse/Rat

£129.50 - £574.50

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Western blot analysis of various cells using PML Polyclonal Antibody diluted at 1：1000

Anti-PML antibody (11-60 aa) (STJ95166)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/IHC/IF/ELISA

Reactivity :

Human/Rat/Mouse

£48.00 - £252.00

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Western blot analysis of extracts from normal (control) and PML knockout (KO) HeLa cells, using PML antibody (STJ25034) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 90s.

Anti-PML antibody (300-600) (STJ25034)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/IHC-P/ELISA

Reactivity :

Human

£129.50 - £642.50

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Western blot analysis of extracts of various cell lines, using PML antibody (STJ11100142) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% non-fat dry milk in TBST. Detection: ECL Enhanced Kit. Exposure time: 3min.

Anti-PML antibody (571-611) (STJ11100142)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/IHC-P/ELISA

Reactivity :

Human/Mouse/Rat

£112.50 - £524.50

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Confocal imaging of C2C12 cells using PMP70/ABCD3 Rabbit monoclonal antibody (STJ11103558, dilution 1:200) followed by a further incubation with Cy3 Goat Anti-Rabbit IgG (H+L) (dilution 1:500) (Red). The cells were counterstained with Alpha-Tubulin Mouse monoclonal antibody ( dilution 1:400) followed by incubation with ABflo® 488-conjugated Goat Anti-Mouse IgG (H+L) antibody ( dilution 1:500) (Green). DAPI was used for nuclear staining (Blue). Objective: 100x.

Anti-PMP70/ABCD3 antibody (560-659) [S8MR] (STJ11103558)

Clonality :

Monoclonal

Host :

Rabbit

Applications :

WB/IF/ICC/ELISA

Reactivity :

Human/Mouse/Rat

£146.50 - £642.50

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Anti-PNOC antibody (20-176) (STJ28478)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/ELISA

Reactivity :

Human

£112.50 - £524.50

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Immunohistochemistry analysis of paraffin-embedded human lung carcinoma tissue, using POFUT1 Antibody. The picture on the right is blocked with the synthesized peptide.

Anti-POFUT1 antibody (331-380) (STJ94602)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/IHC/IF/ELISA

Reactivity :

Human/Mouse/Rat

£48.00 - £252.00

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Immunofluorescence analysis of L929 cells using POGLUT1 Rabbit polyclonal antibody (STJ119778) at dilution of 1:100. Secondary antibody: Cy3 Goat Anti-Rabbit IgG (H+L) at 1:500 dilution. Blue: DAPI for nuclear staining.

Anti-POGLUT1 antibody (160-280) (STJ119778)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/IF/ICC/ELISA

Reactivity :

Mouse/Rat

£112.50 - £524.50

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Anti-POGLUT1 antibody (STJA0009181)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB

Reactivity :

Human/Mouse

£48.00 - £252.00

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