Gene Expression And Transcription | Research Area | St John's Laboratory

Immunofluorescence analysis of Hela cell. 1, c-Myc Polyclonal Antibody (red) was diluted at 1:200 (4°C overnight). CYCS monoclonal antibody (4B10) (green) was diluted at 1:200 (4°C overnight). 2, Goat Anti Rabbit Alexa Fluor 594 Catalog: (NA was diluted at 1:1000 (room temperature, 50min). Goat Anti Mouse Alexa Fluor 488 Catalog: (NA was diluted at 1:1000 (room temperature, 50min).

Anti-CYCS antibody [4B10] (STJ97419)

Clonality :

Monoclonal

Host :

Mouse

Applications :

WB/IF/IHC

Reactivity :

Human/Mouse/Rat/Chicken

£48.00 - £252.00

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Anti-D-Lactic acid-D-Lactic acid-Histone H4-K12 antibody (STJ11107133)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

DB/ELISA

Reactivity :

Human

£129.50 - £642.50

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Western blot analysis of various lysates using Daxx Rabbit polyclonal antibody (STJ11102771) at 1:500 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 180s.

Anti-Daxx antibody (175-400) (STJ11102771)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/ELISA

Reactivity :

Human/Mouse

£112.50 - £524.50

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Western blot analysis of extracts of various cell lines, using Daxx Rabbit monoclonal antibody (STJ11103075) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 180s.

Anti-Daxx antibody (200-300) [S5MR] (STJ11103075)

Clonality :

Monoclonal

Host :

Rabbit

Applications :

WB/ELISA

Reactivity :

Human

£129.50 - £574.50

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Immunocytochemistry analysis of Daxx (green) in K562 using Daxx antibody, and DAPI (blue).

Anti-DAXX antibody (253-272 aa) [R07-7F4] (STJA0012343)

Clonality :

Monoclonal

Host :

Rabbit

Applications :

WB/ICC/IF

Reactivity :

Human

£264.50

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Western blot analysis of various cells using Daxx Polyclonal Antibody

Anti-DAXX antibody (361-410 aa) (STJ92657)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/IHC/IF/ELISA

Reactivity :

Human/Mouse/Rat

£48.00 - £252.00

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Western blot analysis using Daxx monoclonal antibody against K562 cell lysate (1).

Anti-DAXX antibody [7A11] (STJ97995)

Clonality :

Monoclonal

Host :

Mouse

Applications :

WB/IF/FC/ELISA

Reactivity :

Human

£144.00 - £348.00

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Anti-Daxx antibody [DAXX-01] (STJ16100577)

Clonality :

Monoclonal

Host :

Mouse

Applications :

WB/ICC

Reactivity :

Human

£457.50

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Immunohistochemistry analysis of DCR2 in paraffin-embedded mouse brain tissue using DCR2 Rabbit monoclonal antibody (STJ11103526) at a dilution of 1:200 (40x lens). High pressure antigen retrieval was performed with 0. 01 M citrate buffer (pH 6. 0) prior to immunohistochemistry staining.

Anti-DCR2 antibody (287-386) [S6MR] (STJ11103526)

Clonality :

Monoclonal

Host :

Rabbit

Applications :

WB/IHC-P/ELISA

Reactivity :

Human/Mouse/Rat

£129.50 - £574.50

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Immunofluorescence analysis of MCF-7 cells using DDB2 antibody (STJ11104312). Blue: DAPI for nuclear staining.

Anti-DDB2 antibody (1-315) (STJ11104312)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

IF/ICC/ELISA

Reactivity :

Human

£112.50 - £524.50

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Western blot analysis of extracts of various cell lines, using DDB2 antibody (STJ23354) at 1:500 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 90s.

Anti-DDB2 antibody (1-315) (STJ23354)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/IF/ICC/ELISA

Reactivity :

Human/Mouse/Rat

£112.50 - £524.50

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Western blot analysis of extracts of Mouse lung, using DDB2 Rabbit monoclonal antibody (STJ11101370) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 3min.

Anti-DDB2 antibody (220-427) [S0MR] (STJ11101370)

Clonality :

Monoclonal

Host :

Rabbit

Applications :

WB/ELISA

Reactivity :

Human/Mouse

£129.50 - £574.50

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Western blot analysis of lysates from K562 cells, primary antibody was diluted at 1:1000, 4°C over night

Anti-DDB2 antibody (260-340 aa) (STJ191776)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/ELISA

Reactivity :

Human/Mouse

£48.00 - £252.00

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Western blot analysis of lysates from C2C12, using DDIT3/CHOP rabbit polyclonal antibody (STJ116305) at 1:1000 dilution. C2C12 cells were treated by tunicamycin (2 Mu g/ml) for 8 hours. Secondary antibody: HRP Goat Anti-rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% non-fat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 30s.

Anti-DDIT3 antibody (1-169) (STJ116305)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/IHC-P/IF/ICC/ELISA

Reactivity :

Human/Mouse/Rat

£112.50 - £524.50

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Immunofluorescence analysis of U2OS cells using DDIT3/CHOP Rabbit polyclonal antibody (STJ23356) at dilution of 1:100. Blue: DAPI for nuclear staining.

Anti-DDIT3 antibody (1-169) (STJ23356)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/IHC-P/IF/ICC/ELISA

Reactivity :

Human/Mouse/Rat

£112.50 - £524.50

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Immunohistochemical analysis of paraffin-embedded Human Stomach Carcinoma Tissue using CHOP Mouse mAb diluted at 1:200.

Anti-DDIT3 antibody (10-90 aa) [1E1] (STJ97775)

Clonality :

Monoclonal

Host :

Mouse

Applications :

IHC/IF

Reactivity :

Human/Rat/Mouse

£48.00 - £252.00

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Immunofluorescence analysis of Hela cell. 1, Calnexin Polyclonal Antibody (green) was diluted at 1:200 (4°C overnight). (red) was diluted at 1:200 (4°C overnight). 2, Goat Anti Rabbit Alexa Fluor 488 Catalog: (NA was diluted at 1:1000 (room temperature, 50min). Goat Anti Mouse Alexa Fluor 594 Catalog: (NA was diluted at 1:1000 (room temperature, 50min).

Anti-DDIT3 antibody (10-90 aa) [2B1] (STJ97774)

Clonality :

Monoclonal

Host :

Mouse

Applications :

WB/IF/IHC

Reactivity :

Human/Rat/Mouse

£48.00 - £252.00

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Western blot analysis of various cells using CHOP Polyclonal Antibody diluted at 1：500

Anti-DDIT3 antibody (15-64 aa) (STJ92277)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/IHC/IF/ELISA

Reactivity :

Human/Mouse

£48.00 - £252.00

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Western blot analysis of extracts of C6 cells, using DDIT3/CHOP antibody (STJ113090) at 1:1000 dilution. C6 cells were treated by tunicamycin (2 Mu g/ml) for 8 hours. Secondary antibody: HRP Goat Anti-rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% non-fat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 90s.

Anti-DDIT3 antibody (39-169) (STJ113090)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/IHC-P/IP/ELISA

Reactivity :

Mouse/Rat

£112.50 - £524.50

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Western blot with Anti-DDIT3 Antibody (STJ501130) and peptide. At 1:500 dilution in buffer on a 14% SDS gel.

Anti-DDIT3 antibody (60-160) (STJ501130)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

ELISA/IP/WB

Reactivity :

Human/Mouse/Rat

£427.50

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Anti-DDIT3 antibody (60-160) {Biotin} (STJ501131)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

ELISA/IP/WB

Reactivity :

Human/Mouse/Rat

£528.50

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Anti-DDIT3 antibody (60-160) {FITC} (STJ501132)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

ELISA/IP/WB

Reactivity :

Human/Mouse/Rat

£528.50

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Anti-DDIT3/CHOP antibody (1-100 aa) [SRM] (STJ11106862)

Clonality :

Monoclonal

Host :

Rabbit

Applications :

WB/IHC-P/ELISA

Reactivity :

Human/Mouse/Rat

£129.50 - £574.50

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Western blot analysis of lysates from wild type (WT) and DDIT4 knockout (KO) HeLa cells, using [KO Validated] DDIT4 Rabbit pAb (STJ110386) at 1:1000 dilution. <br/>Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. <br/>Lysates/proteins: 25 Mu g per lane. <br/>Blocking buffer: 3% nonfat dry milk in TBST. <br/>Detection: ECL Enhanced Kit <br/>Exposure time: 90s.

Anti-DDIT4 antibody (1-232) (STJ110386)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/ELISA

Reactivity :

Human

£129.50 - £642.50

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Western blot analysis of extracts of various cell lines, using DDIT4 antibody (STJ116070) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 60s.

Anti-DDIT4 antibody (1-232) (STJ116070)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/ELISA

Reactivity :

Human/Mouse

£112.50 - £524.50

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Anti-DDIT4 antibody (STJA0006731)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB

Reactivity :

Human/Mouse/Rat

£48.00 - £252.00

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Immunohistochemistry analysis of paraffin-embedded mouse testis using DDX21 antibody (STJ117020) at dilution of 1:100 (40x lens). Perform microwave antigen retrieval with 10 mM PBS buffer pH 7. 2 before commencing with immunohistochemistry staining protocol.

Anti-DDX21 antibody (1-180) (STJ117020)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/IHC-P/IF/ICC/ELISA

Reactivity :

Human/Mouse

£112.50 - £524.50

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Immunocytochemistry analysis of DDX21 (green) in 293T using DDX21 antibody, and DAPI (blue).

Anti-DDX21 antibody (248-260 aa) [R04-4J2] (STJA0011226)

Clonality :

Monoclonal

Host :

Rabbit

Applications :

WB/ICC/IF

Reactivity :

Human

£246.50

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Western blot analysis of lysates from VEC cells, primary antibody was diluted at 1:1000, 4°C over night

Anti-DDX21 antibody (287-337 aa) (STJ195748)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB

Reactivity :

Human/Mouse/Rat

£48.00 - £252.00

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Western blot analysis of extracts of various cell lines, using DDX21 antibody (STJ29114) at 1:1000 dilution. _Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. _Lysates/proteins: 25 Mu g per lane. _Blocking buffer: 3% nonfat dry milk in TBST. _Detection: ECL Enhanced Kit. _Exposure time: 60s.

Anti-DDX21 antibody (624-783) (STJ29114)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/ELISA

Reactivity :

Human/Mouse

£112.50 - £524.50

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Western blot analysis of various lysates using DDX39A Rabbit pAb (STJ110264) at 1:1000 dilution. <br/>Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. <br/>Lysates/proteins: 25 Mu g per lane. <br/>Blocking buffer: 3% nonfat dry milk in TBST. <br/>Detection: ECL Basic Kit <br/>Exposure time: 90s.

Anti-DDX39A antibody (1-100) (STJ110264)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/IHC-P/IF/ICC/ELISA

Reactivity :

Human/Mouse/Rat

£112.50 - £524.50

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Western blot analysis of extracts of various cell lines, using DDX39A antibody (STJ114432) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 90s.

Anti-DDX39A antibody (178-427) (STJ114432)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/ELISA

Reactivity :

Mouse/Rat

£112.50 - £524.50

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Anti-DDX39A antibody (STJA0007477)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB

Reactivity :

Human/Mouse/Rat

£48.00 - £252.00

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Western blot analysis of various lysates using UAP56/DDX39B Rabbit mAb (STJ11102538) at 1:1000 dilution. <br/>Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. <br/>Lysates/proteins: 25 Mu g per lane. <br/>Blocking buffer: 3% nonfat dry milk in TBST. <br/>Detection: ECL Basic Kit <br/>Exposure time: 10s.

Anti-DDX39B antibody (310-428) [S8MR] (STJ11102538)

Clonality :

Monoclonal

Host :

Rabbit

Applications :

WB/IHC-P/ELISA

Reactivity :

Human/Mouse/Rat

£129.50 - £574.50

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Western blot analysis of various lysates using UAP56/DDX39B Rabbit pAb (STJ110654) at 1:1000 dilution. <br/>Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. <br/>Lysates/proteins: 25 Mu g per lane. <br/>Blocking buffer: 3% nonfat dry milk in TBST. <br/>Detection: ECL Basic Kit <br/>Exposure time: 5s.

Anti-DDX39B antibody (319-428) (STJ110654)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/IHC-P/IF/ICC/ELISA

Reactivity :

Human/Mouse/Rat

£112.50 - £524.50

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Anti-DDX39B antibody (STJA0007045)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB

Reactivity :

Human/Mouse/Rat

£48.00 - £252.00

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Anti-DDX4 antibody (550-600 aa) (STJ13101035)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

IHC/WB

Reactivity :

Human/Rat/Mouse

£331.50

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Western blot analysis of extracts of mouse testis, using DDX4 antibody (STJ118072) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% non-fat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 10s.

Anti-DDX4 antibody (635-724) (STJ118072)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/IHC-P/IF/ICC/ELISA

Reactivity :

Mouse/Rat

£112.50 - £524.50

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Western blot analysis using DDX4 monoclonal antibody against DDX4-hIgGFc transfected HEK293 cell lysate.

Anti-DDX4 antibody [2F9H5] (STJ97998)

Clonality :

Monoclonal

Host :

Mouse

Applications :

WB/IHC/IF/FC/ELISA

Reactivity :

Human

£144.00 - £348.00

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Anti-DDX4/Vasa antibody [SAA1970] (STJA0020398)

Clonality :

Monoclonal

Host :

Mouse

Applications :

ELISA

Reactivity :

Human

£341.50 - £1,447.50

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Immunohistochemistry of mouse tissue with Anti-DEK primary antibody (STJ500750) at 1:50 dilution in Buffer. Section was treated with DAB and Haematoxylin stain. Magnification is at 20X.

Anti-DEK antibody (324-373) (STJ500750)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

ELISA/IHC/IP/WB

Reactivity :

Bovine/Cat/Human/Monkey/Mouse/Rat

£427.50

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Anti-DEK antibody (324-373) {Biotin} (STJ500751)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

ELISA/IHC/IP/WB

Reactivity :

Bovine/Cat/Human/Monkey/Mouse/Rat

£528.50

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Anti-DEK antibody (324-373) {FITC} (STJ500752)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

ELISA/IHC/IP/WB

Reactivity :

Bovine/Cat/Human/Monkey/Mouse/Rat

£528.50

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Immunofluorescence analysis of C6 cells using DEK Rabbit monoclonal antibody (STJ11102678) at dilution of 1:100 (40x lens). Blue: DAPI for nuclear staining.

Anti-DEK antibody (51-200) [S8MR] (STJ11102678)

Clonality :

Monoclonal

Host :

Rabbit

Applications :

WB/IHC-P/IF/ICC/ELISA

Reactivity :

Human/Rat

£129.50 - £574.50

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Anti-DEK antibody (STJA0006779)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB

Reactivity :

Human/Mouse/Rat

£48.00 - £252.00

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Immunoprecipitation analysis of 300 Mu g extracts of HeLa cells using 3 Mu g DGCR8 antibody (STJ11100564). Western blot was performed from the immunoprecipitate using DGCR8 antibody (STJ11100564) at a dilution of 1:1000.

Anti-DGCR8 antibody (180-340) (STJ11100564)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/IHC-P/IP/ELISA

Reactivity :

Human/Mouse/Rat

£112.50 - £524.50

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Immunocytochemistry analysis of DGCR8 (green) in K562 using DGCR8 antibody, and DAPI (blue).

Anti-DGCR8 antibody (424-773 aa) [R05-5A6] (STJA0011234)

Clonality :

Monoclonal

Host :

Rabbit

Applications :

WB/ICC/IF/IP

Reactivity :

Human/Mouse/Rat

£264.50

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Western blot analysis of extracts of Mouse brain, using DGCR8 Rabbit monoclonal antibody (STJ11101977) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 3min.

Anti-DGCR8 antibody (436-764) [S7MR] (STJ11101977)

Clonality :

Monoclonal

Host :

Rabbit

Applications :

WB/ELISA

Reactivity :

Human/Mouse

£129.50 - £574.50

Choose Options