Extracellular Matrix Organisation | Research Area | St John's Laboratory

Whole cell lysates were separated by 10% SDS-PAGE, and the membrane was blotted with anti-CD61 (ABT032) antibody. The HRP-conjugated Goat anti- mouse IgG (H + L) antibody was used to detect the antibody. Lane 1: HUVECPredicted band size: 70kDaObserved band size: 70kDa

Anti-ITGB3 antibody (1-100 aa) [PT0052] (STJ196789)

Clonality :

Monoclonal

Host :

Mouse

Applications :

IHC/WB/IF

Reactivity :

Human/Mouse/Rat

£174.00 - £252.00

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Immunohistochemistry of baboon kidney with Anti-ITGB3 primary antibody (STJ501482) at 1:100 dilution in Buffer. Section was treated with DAB and Haematoxylin stain. Magnification is at 40X.

Anti-ITGB3 antibody (1-50) (STJ501482)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

ELISA/IHC/IP/WB

Reactivity :

Human/Monkey

£427.50

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Anti-ITGB3 antibody (1-50) {Biotin} (STJ501483)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

ELISA/IHC/IP/WB

Reactivity :

Human/Monkey

£528.50

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Anti-ITGB3 antibody (1-50) {FITC} (STJ501484)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

ELISA/IHC/IP/WB

Reactivity :

Human/Monkey

£528.50

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Western blot analysis of various lysates using ITGB3 Rabbit polyclonal antibody (STJ11105487) at 1:900 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/ proteins: 25 Mu g per lane. Blocking buffer: 3 % nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 90s.

Anti-ITGB3 antibody (21-718) (STJ11105487)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/FC/ELISA

Reactivity :

Human/Mouse/Rat

£112.50 - £524.50

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Western blot analysis of extracts of various cell lines, using Integrin beta 3 (ITGB3/CD61) antibody (STJ114863) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% non-fat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 60s.

Anti-ITGB3 antibody (609-788) (STJ114863)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/ELISA

Reactivity :

Human/Mouse

£112.50 - £524.50

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Western blot analysis of extracts of various cell lines, using Integrin beta 3 (ITGB3/CD61) antibody (STJ11101610) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% non-fat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 90s.

Anti-ITGB3 antibody (689-788) [S0MR] (STJ11101610)

Clonality :

Monoclonal

Host :

Rabbit

Applications :

WB/IHC-P/IP/FC/ELISA

Reactivity :

Human/Mouse/Rat

£146.50 - £642.50

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Whole cell lysates of A549 were separated by 10% SDS-PAGE, and the membrane was blotted with anti-Integrin β3 (PTR2555) antibody. The HRP-conjugated Goat anti- mouse IgG (H + L) antibody was used to detect the antibody. Lane 1: A549Predicted band size: 87kDaObserved band size: 72kDa

Anti-ITGB3 antibody (700-788) [PTR2555] (STJA0006262)

Clonality :

Monoclonal

Host :

Mouse

Applications :

WB/ELISA

Reactivity :

Human/Mouse/Rat

£48.00 - £252.00

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Western blot analysis of various cells using Integrin Beta 3 Polyclonal Antibody diluted at 1：500

Anti-ITGB3 antibody (739-788 aa) (STJ93735)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/IHC/IF/ELISA

Reactivity :

Human/Mouse/Rat

£48.00 - £252.00

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Immunohistochemistry analysis of paraffin-embedded mouse bone marrow using Integrin beta 3 (ITGB3/CD61) Rabbit polyclonal antibody (STJ26210) at dilution of 1:100 (40x lens). Perform high pressure antigen retrieval with 10 mM citrate buffer pH 6. 0 before commencing with immunohistochemistry staining protocol.

Anti-ITGB3 antibody (80-788) (STJ26210)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/IHC-P/ELISA

Reactivity :

Human/Mouse/Rat

£112.50 - £524.50

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Anti-ITGB3 antibody (Extracellular region) [M581] (STJA0005450)

Clonality :

Monoclonal

Host :

Mouse

Applications :

WB/ICC

Reactivity :

Human/Mouse/Rat

£365.50

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Western blot detection of Integrin beta 3 (N-terminus) in 15ug and 25ug Leukocyte whole cell lysate using Integrin beta 3 (N-terminus) mouse mAb (1:1000 diluted).Predicted band size:110KDa.Observed band size:110KDa.

Anti-ITGB3 antibody (N-Term) [3C12-G2-E4-H9] (STJ99085)

Clonality :

Monoclonal

Host :

Mouse

Applications :

WB

Reactivity :

Human

£144.00 - £348.00

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Anti-ITGB3 antibody [ABT134R] (STJ197069)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

IHC/IF

Reactivity :

Human

£174.00 - £252.00

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Western blot analysis of various cells using Integrin Beta 4 Polyclonal Antibody

Anti-ITGB4 antibody (1481-1530 aa) (STJ93737)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/IHC/IF/ELISA

Reactivity :

Human/Mouse/Rat

£48.00 - £252.00

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Western blot analysis of extracts of Mouse brain, using Integrin beta 4 (ITGB4/CD104) Rabbit monoclonal antibody (STJ11102113) at 1:500 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Enhanced Kit. Exposure time: 3min.

Anti-ITGB4 antibody (1604-1822) [S3MR] (STJ11102113)

Clonality :

Monoclonal

Host :

Rabbit

Applications :

WB/ELISA

Reactivity :

Human/Mouse

£129.50 - £574.50

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Western blot analysis of various lysates, using Integrin beta 4 (ITGB4/CD104) Rabbit polyclonal antibody (STJ24260) at 1:900 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 90s.

Anti-ITGB4 antibody (611-710) (STJ24260)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/ELISA

Reactivity :

Human/Mouse/Rat

£112.50 - £524.50

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Anti-ITGB4 antibody (Cytoplasmic) [M126] (STJA0005451)

Clonality :

Monoclonal

Host :

Mouse

Applications :

WB/ICC

Reactivity :

Human

£390.50

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Western blot analysis of extracts from A549, using Integrin Beta 5/ITGB5 Rabbit monoclonal antibody (STJ11105106) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 30s.

Anti-ITGB5 antibody (24-490 aa) [S5106RM] (STJ11105106)

Clonality :

Monoclonal

Host :

Rabbit

Applications :

WB/ELISA

Reactivity :

Human

£129.50 - £574.50

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Western blot analysis of lysates from A549 cells, using ITGB5 Rabbit polyclonal antibody (STJ24261) at 1:2000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 90s.

Anti-ITGB5 antibody (490-720) (STJ24261)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/ELISA

Reactivity :

Human/Mouse/Rat

£112.50 - £524.50

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Western blot analysis of various cells using Integrin Beta 5 Polyclonal Antibody diluted at 1：1000

Anti-ITGB5 antibody (721-770 aa) (STJ93738)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/ELISA

Reactivity :

Human/Mouse/Rat/Monkey

£48.00 - £252.00

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Western blot analysis of various lysates using ITGB6 Rabbit polyclonal antibody (STJ119226) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 15s.

Anti-ITGB6 antibody (732-788) (STJ119226)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/ELISA

Reactivity :

Human/Mouse/Rat

£112.50 - £524.50

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Anti-ITGB6 antibody (STJ191874)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/ELISA

Reactivity :

Human/Mouse/Rat

£48.00 - £252.00

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Western blot analysis of various lysates using ITGB7 Rabbit polyclonal antibody (STJ27701) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 90s.

Anti-ITGB7 antibody (1-140) (STJ27701)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/ELISA

Reactivity :

Human/Mouse

£112.50 - £524.50

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Western blot analysis of mouse-heart cells using primary antibody diluted at 1:1000 (4°C overnight). Secondary antibody：Goat Anti-rabbit IgG IRDye 800 ( diluted at 1:5000, 25°C, 1 hour)

Anti-ITGB7 antibody (671-720 aa) (STJ99322)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/ELISA/IHC

Reactivity :

Human/Mouse

£48.00 - £252.00

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Anti-ITGB7 antibody [SAA1982] (STJA0020410)

Clonality :

Monoclonal

Host :

Mouse

Applications :

ELISA

Reactivity :

Human

£341.50 - £1,447.50

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Western blot analysis of various lysates, using ITGB8 Rabbit pAb (STJ110731) at 1:500 dilution. <br/>Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. <br/>Lysates/proteins: 25 Mu g per lane. <br/>Blocking buffer: 3% nonfat dry milk in TBST. <br/>Detection: ECL Basic Kit <br/>Exposure time: 10s.

Anti-ITGB8 antibody (43-320) (STJ110731)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/ELISA

Reactivity :

Human/Mouse/Rat

£112.50 - £524.50

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Anti-ITGB8 antibody (STJ191875)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/ELISA

Reactivity :

Human/Rat/Mouse

£48.00 - £252.00

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Anti-J1 epitope of COL2A1 antibody [M2139] (STJA0019067)

Clonality :

Monoclonal

Host :

Mouse

Applications :

ELISA/FC/IHC/WB

Reactivity :

Human/Mouse/Rat/Bovine

£378.50 - £1,632.50

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Anti-JAM-1 antibody [BV12] (STJ16100185)

Clonality :

Monoclonal

Host :

Mouse

Applications :

FC/IF/IP/WB

Reactivity :

Mouse

£802.50

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Anti-JAM-1 antibody [BV16] (STJ16100272)

Clonality :

Monoclonal

Host :

Mouse

Applications :

IHC-F/FC/IF/IP/WB

Reactivity :

Human

£883.50

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Anti-JAM-1 antibody [M.Ab.F11] (STJ16100273)

Clonality :

Monoclonal

Host :

Mouse

Applications :

FC/ELISA/IF/IP/IHC-P/WB

Reactivity :

Human

£883.50

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Anti-JAM-A/CD321/F11R antibody (28-242 aa) [SRM] (STJ11107263)

Clonality :

Monoclonal

Host :

Rabbit

Applications :

WB/ELISA

Reactivity :

Human

£129.50 - £574.50

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Western blot analysis of various lysates using JAM2 Rabbit pAb (STJ112019) at 1:1000 dilution. <br/>Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. <br/>Lysates/proteins: 25 Mu g per lane. <br/>Blocking buffer: 3% nonfat dry milk in TBST. <br/>Detection: ECL Basic Kit <br/>Exposure time: 90s.

Anti-JAM2 antibody (29-238) (STJ112019)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/ELISA

Reactivity :

Human/Mouse/Rat

£112.50 - £524.50

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Western blot analysis of K562 cells using JAM-B Polyclonal Antibody.. Secondary antibody was diluted at 1:20000

Anti-JAM2 antibody (Internal) (STJ96480)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/IHC/IF/ELISA

Reactivity :

Human/Mouse/Rat

£48.00 - £252.00

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Western blot analysis of lysates from HCT116 cells, primary antibody was diluted at 1:1000, 4°C over night

Anti-JAM3 antibody (60-140 aa) (STJ190344)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/ELISA

Reactivity :

Human/Mouse/Rat

£48.00 - £252.00

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Anti-JAM3 antibody [SAA1837] (STJA0020265)

Clonality :

Monoclonal

Host :

Mouse

Applications :

ELISA

Reactivity :

Human

£341.50 - £1,447.50

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Western blot analysis of extracts of A-549 cells, using VEGF Receptor 2 antibody (STJ112973) at 1:500 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 60s.

Anti-KDR antibody (1147-1356) (STJ112973)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/ELISA

Reactivity :

Human/Mouse/Rat

£112.50 - £524.50

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Western blot analysis of lysates from Mouse heart, using VEGF Receptor 2 Rabbit pAb (STJ28277) at 1:1000 dilution. <br/>Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. <br/>Lysates/proteins: 25 Mu g per lane. <br/>Blocking buffer: 3% nonfat dry milk in TBST. <br/>Detection: ECL Enhanced Kit <br/>Exposure time: 180s.

Anti-KDR antibody (1150-1250) (STJ28277)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/ELISA

Reactivity :

Human/Mouse/Rat

£112.50 - £524.50

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Immunohistochemical analysis of paraffin-embedded human-breast-cancer, antibody was diluted at 1:200

Anti-KDR antibody (1268-1341 aa) (STJ98952)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

IF/WB/IHC/ELISA

Reactivity :

Human

£48.00 - £252.00

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Anti-VEGFR2 antibody at 1:500 dilution; lysates at 50µg per lane; rabbit polyclonal to goat IgG (HRP) at 1:10000 dilution

Anti-KDR antibody (20-125aa) (STJ140092)

Clonality :

Polyclonal

Host :

Goat

Applications :

WB/IF

Reactivity :

Human/Rat/Mouse/Monkey

£101.50 - £394.50

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Western blot analysis using Flk-1 monoclonal antibody against extracellular domain of human Flk-1 (aa20-764).

Anti-KDR antibody (20-764 aa) [4B4] (STJ98079)

Clonality :

Monoclonal

Host :

Mouse

Applications :

WB/ChIP/IF/FC/ELISA

Reactivity :

Human/Fish

£144.00 - £348.00

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Western blot analysis of lysates from wild type (WT) and 293F cells transfected with VEGFR2/CD309 using VEGFR2/CD309 Rabbit mAb (STJ11105664) at 1:1000 dilution incubated overnight at 4C. <br>Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. <br>Lysates/proteins: 25 Mu g per lane. <br>Blocking buffer: 3% nonfat dry milk in TBST. <br>Detection: ECL Basic Kit ( Exposure time: 30s.

Anti-KDR antibody (20-764) [S5664RM] (STJ11105664)

Clonality :

Monoclonal

Host :

Rabbit

Applications :

WB/ELISA

Reactivity :

Human

£129.50 - £574.50

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Immunofluorescence analysis of rat-lung tissue. 1, Flk-1 Polyclonal Antibody (red) was diluted at 1:200 (4°C, overnight). 2, Cy3 labled Secondary antibody was diluted at 1:300 (room temperature, 50min).3, Picture B: DAPI (blue) 10min. Picture A:Target. Picture B: DAPI. Picture C: merge of A+B

Anti-KDR antibody (917-966 aa) (STJ93088)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/IHC/IF/ELISA

Reactivity :

Human/Mouse/Fish

£48.00 - £252.00

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Anti-KDR antibody (N-Term) (STJA0005640)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/ICC

Reactivity :

Human/Mouse/Rat

£319.50

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Western blot analysis of extracts of various cell lines, using KLK7 antibody (STJ27457) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 90s.

Anti-KLK7 antibody (30-253) (STJ27457)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/ELISA

Reactivity :

Human/Mouse

£112.50 - £524.50

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Anti-KLK7 antibody [SAA0869] (STJA0019664)

Clonality :

Monoclonal

Host :

Mouse

Applications :

Blocking/ELISA

Reactivity :

Human

£378.50 - £1,632.50

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Immunohistochemistry analysis of paraffin-embedded human placenta using KLKB1 antibody (STJ27271) at dilution of 1:100 (40x lens). Perform microwave antigen retrieval with 10 mM PBS buffer pH 7. 2 before commencing with immunohistochemistry staining protocol.

Anti-KLKB1 antibody (20-300) (STJ27271)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/IHC-P/ELISA

Reactivity :

Human/Mouse/Rat

£112.50 - £524.50

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Immunohistochemistry analysis of paraffin-embedded mouse lung using KLKB1 antibody (STJ115285) at dilution of 1:200 (40x lens). Perform microwave antigen retrieval with 10 mM PBS buffer pH 7. 2 before commencing with immunohistochemistry staining protocol.

Anti-KLKB1 antibody (391-500) (STJ115285)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/IHC-P/ELISA

Reactivity :

Human/Mouse/Rat

£112.50 - £524.50

Choose Options