| Applications: |
CLIA |
| Reactivity: |
Rat |
| Note: |
FOR SCIENTIFIC EDUCATIONAL RESEARCH USE ONLY (RUO). MUST NOT BE USED IN DIAGNOSTIC OR OTHER MEDICAL APPLICATIONS. |
| Sensitivity: |
0.19ng/mL |
| Detection Limit: |
0.31~20ng/mL |
| Short Description: |
This rat MIF kit is a highly sensitive in-vitro chemiluminescent immunoassay for the measurement of trace amounts of analytes. |
| Storage Instruction: |
If unopened the kit may be stored at 2-8°C for up to 1 month. If the kit will not be used within 1 month, store the components separately, according to the component table in the manual. |
| Assay Time: |
3.5h |
| Detection: |
Chemiluminescence |
| Gene Symbol: |
Mif |
| Gene ID: |
81683 |
| Uniprot ID: |
MIF_RAT |
| Specificity: |
This kit recognizes Rat MIF in samples. No significant cross-reactivity or interference between Rat MIF and analogues was observed. |
| Sample Type: |
Serum, plasma and other biological fluids |
| Tissue Specificity | Expressed in a wide variety of organs including brain, spleen, liver, muscle and kidney. |
| Function | Pro-inflammatory cytokine involved in the innate immune response to bacterial pathogens. The expression of MIF at sites of inflammation suggests a role as mediator in regulating the function of macrophages in host defense. Counteracts the anti-inflammatory activity of glucocorticoids. Has phenylpyruvate tautomerase and dopachrome tautomerase activity (in vitro), but the physiological substrate is not known. It is not clear whether the tautomerase activity has any physiological relevance, and whether it is important for cytokine activity. |
| Protein Name | Macrophage Migration Inhibitory FactorMifGlutathione-Binding 13 Kda ProteinL-Dopachrome IsomeraseL-Dopachrome TautomerasePhenylpyruvate Tautomerase |
| Database Links | Reactome: R-RNO-202733Reactome: -RNO-6798695 |
| Cellular Localisation | SecretedCytoplasmDoes Not Have A Cleavable Signal Sequence And Is Secreted Via A SpecializedNon-Classical PathwaySecreted By Macrophages Upon Stimulation By Bacterial Lipopolysaccharide (Lps)Or By MTuberculosis Antigens |
| Alternative CLIA Names | Macrophage Migration Inhibitory Factor CLIA kitMif CLIA kitGlutathione-Binding 13 Kda Protein CLIA kitL-Dopachrome Isomerase CLIA kitL-Dopachrome Tautomerase CLIA kitPhenylpyruvate Tautomerase CLIA kitMif CLIA kit |
| Specificity | This kit recognizes Rat MIF in samples. No significant cross-reactivity or interference between Rat MIF and analogues was observed. |
| Reproducibility | Both intra-CV and inter-CV are |
Information sourced from Uniprot.org
| Item | Specifications | Storage |
| Micro CLIA Plate (Dismountable) | 96T: 8 wells ×12 strips strips | -20℃, 6 months |
| Reference Standard | 96T: 2 vials 48T: 1 vial | -20℃, 6 months |
| Concentrated Biotinylated Detection Ab (100×) | 96T: 1 vial, 120 μL 60 μL | -20℃, 6 months |
| Concentrated HRP Conjugate (100×) | 96T: 1 vial, 120 μL 60 μL | -20℃ (Protect from light), 6 months |
| Reference Standard & Sample Diluent | 1 vial, 20 mL | 2-8°C, 6 months |
| Biotinylated Detection Ab Diluent | 1 vial, 14 mL | 2-8°C, 6 months |
| HRP Conjugate Diluent | 1 vial, 14 mL | 2-8°C, 6 months |
| Concentrated Wash Buffer (25×) | 1 vial, 30 mL | 2-8°C, 6 months |
| Substrate Reagent A | 1 vial, 5 mL | 2-8℃ (Protect from light) |
| Substrate Reagent B | 1 vial, 5 mL | 2-8℃ (Protect from light) |
| Plate Sealer | 5 pieces | |
| Manual | 1 copy | |
| Certificate of Analysis | 1 copy | |
| Sample Type | Range (%) | Average Recovery (%) |
| Serum(n=8) | 88-103 | 94 |
| EDTA plasma(n=8) | 99-110 | 105 |
| Cell culture media(n=8) | 96-108 | 103 |
| | Intra-assay Precision | Intra-assay Precision | Intra-assay Precision | Inter-assay Precision | Inter-assay Precision | Inter-assay Precision |
| Sample | 1.00 | 2.00 | 3.00 | 1.00 | 2.00 | 3.00 |
| n | 20.00 | 20.00 | 20.00 | 20.00 | 20.00 | 20.00 |
| Mean (ng/mL) | 1.04 | 3.05 | 6.98 | 1.13 | 3.03 | 7.18 |
| Standard deviation | 0.12 | 0.33 | 0.69 | 0.09 | 0.35 | 0.70 |
| CV (%) | 11.54 | 10.82 | 9.89 | 7.96 | 11.55 | 9.75 |
12 months for antibodies. 6 months for ELISA Kits. Please see website T&Cs for further guidance
