• Rat LIF (Leukemia Inhibitory Factor) CLIA Kit (STJC0001782)

Rat LIF (Leukemia Inhibitory Factor) CLIA Kit (STJC0001782)

SKU:
STJC0001782

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Applications: CLIA
Reactivity: Rat
Note: FOR SCIENTIFIC EDUCATIONAL RESEARCH USE ONLY (RUO). MUST NOT BE USED IN DIAGNOSTIC OR OTHER MEDICAL APPLICATIONS.
Sensitivity: 4.69pg/mL
Detection Limit: 7.81~500pg/mL
Short Description: This rat LIF kit is a highly sensitive in-vitro chemiluminescent immunoassay for the measurement of trace amounts of analytes.
Storage Instruction: If unopened the kit may be stored at 2-8°C for up to 1 month. If the kit will not be used within 1 month, store the components separately, according to the component table in the manual.
Assay Time: 3.5h
Detection: Chemiluminescence
Gene Symbol: LifUniprot ID=LIF_RAT"
Specificity: This kit recognizes Rat LIF in samples. No significant cross-reactivity or interference between Rat LIF and analogues was observed.
Sample Type: Serum, plasma and other biological fluids
Function LIF has the capacity to induce terminal differentiation in leukemic cells. Its activities include the induction of hematopoietic differentiation in normal and myeloid leukemia cells, the induction of neuronal cell differentiation, and the stimulation of acute-phase protein synthesis in hepatocytes.
Protein Name Leukemia Inhibitory Factor
Lif
Cholinergic Neuronal Differentiation Factor
Cellular Localisation Secreted
Alternative CLIA Names Leukemia Inhibitory Factor CLIA kit
Lif CLIA kit
Cholinergic Neuronal Differentiation Factor CLIA kit
Lif CLIA kit
Specificity This kit recognizes Rat LIF in samples. No significant cross-reactivity or interference between Rat LIF and analogues was observed.
Reproducibility Both intra-CV and inter-CV are

Information sourced from Uniprot.org


Item Specifications Storage
Micro CLIA Plate (Dismountable) 96T: 8 wells ×12 strips strips -20℃, 6 months
Reference Standard 96T: 2 vials 48T: 1 vial -20℃, 6 months
Concentrated Biotinylated Detection Ab (100×) 96T: 1 vial, 120 μL 60 μL -20℃, 6 months
Concentrated HRP Conjugate (100×) 96T: 1 vial, 120 μL 60 μL -20℃ (Protect from light), 6 months
Reference Standard & Sample Diluent 1 vial, 20 mL 2-8°C, 6 months
Biotinylated Detection Ab Diluent 1 vial, 14 mL 2-8°C, 6 months
HRP Conjugate Diluent 1 vial, 14 mL 2-8°C, 6 months
Concentrated Wash Buffer (25×) 1 vial, 30 mL 2-8°C, 6 months
Substrate Reagent A 1 vial, 5 mL 2-8℃ (Protect from light)
Substrate Reagent B 1 vial, 5 mL 2-8℃ (Protect from light)
Plate Sealer 5 pieces
Manual 1 copy
Certificate of Analysis 1 copy

Sample Type Range (%) Average Recovery (%)
Serum(n=8) 100-113 106
EDTA plasma(n=8) 100-113 105
Cell culture media(n=8) 85-98 90

Intra-assay Precision Intra-assay Precision Intra-assay Precision Inter-assay Precision Inter-assay Precision Inter-assay Precision
Sample 1.00 2.00 3.00 1.00 2.00 3.00
n 20.00 20.00 20.00 20.00 20.00 20.00
Mean (pg/mL) 22.94 45.59 238.15 21.57 42.85 261.24
Standard deviation 2.27 4.37 16.74 1.73 4.37 28.19
CV (%) 9.90 9.59 7.03 8.02 10.20 10.79
12 months for antibodies. 6 months for ELISA Kits. Please see website T&Cs for further guidance