| Applications: |
CLIA |
| Reactivity: |
Rat |
| Note: |
FOR SCIENTIFIC EDUCATIONAL RESEARCH USE ONLY (RUO). MUST NOT BE USED IN DIAGNOSTIC OR OTHER MEDICAL APPLICATIONS. |
| Sensitivity: |
75pg/mL |
| Detection Limit: |
125~8000pg/mL |
| Short Description: |
This rat F10 kit is a highly sensitive in-vitro chemiluminescent immunoassay for the measurement of trace amounts of analytes. |
| Storage Instruction: |
If unopened the kit may be stored at 2-8°C for up to 1 month. If the kit will not be used within 1 month, store the components separately, according to the component table in the manual. |
| Assay Time: |
3.5h |
| Detection: |
Chemiluminescence |
| Gene Symbol: |
F10 |
| Gene ID: |
29243 |
| Uniprot ID: |
FA10_RAT |
| Specificity: |
This kit recognizes Rat F10 in samples. No significant cross-reactivity or interference between Rat F10 and analogues was observed. |
| Sample Type: |
Serum, plasma and other biological fluids |
| Tissue Specificity | Plasma.synthesized in the liver. |
| Post Translational Modifications | The vitamin K-dependent, enzymatic carboxylation of some glutamate residues allows the modified protein to bind calcium. N- and O-glycosylated. Proteolytically cleaved and activated by cathepsin CTSG. The activation peptide is cleaved by factor IXa (in the intrinsic pathway), or by factor VIIa (in the extrinsic pathway). The iron and 2-oxoglutarate dependent 3-hydroxylation of aspartate and asparagine is (R) stereospecific within EGF domains. |
| Function | Factor Xa is a vitamin K-dependent glycoprotein that converts prothrombin to thrombin in the presence of factor Va, calcium and phospholipid during blood clotting. |
| Protein Name | Coagulation Factor XStuart Factor Cleaved Into - Factor X Light Chain - Factor X Heavy Chain - Activated Factor Xa Heavy Chain |
| Database Links | Reactome: R-RNO-140834Reactome: -RNO-140837Reactome: -RNO-140875Reactome: -RNO-159740Reactome: -RNO-159763Reactome: -RNO-159782 |
| Cellular Localisation | Secreted |
| Alternative CLIA Names | Coagulation Factor X CLIA kitStuart Factor Cleaved Into - Factor X Light Chain - Factor X Heavy Chain - Activated Factor Xa Heavy Chain CLIA kitF10 CLIA kit |
| Specificity | This kit recognizes Rat F10 in samples. No significant cross-reactivity or interference between Rat F10 and analogues was observed. |
| Reproducibility | Both intra-CV and inter-CV are |
Information sourced from Uniprot.org
| Item | Specifications | Storage |
| Micro CLIA Plate (Dismountable) | 96T: 8 wells ×12 strips strips | -20℃, 6 months |
| Reference Standard | 96T: 2 vials 48T: 1 vial | -20℃, 6 months |
| Concentrated Biotinylated Detection Ab (100×) | 96T: 1 vial, 120 μL 60 μL | -20℃, 6 months |
| Concentrated HRP Conjugate (100×) | 96T: 1 vial, 120 μL 60 μL | -20℃ (Protect from light), 6 months |
| Reference Standard & Sample Diluent | 1 vial, 20 mL | 2-8°C, 6 months |
| Biotinylated Detection Ab Diluent | 1 vial, 14 mL | 2-8°C, 6 months |
| HRP Conjugate Diluent | 1 vial, 14 mL | 2-8°C, 6 months |
| Concentrated Wash Buffer (25×) | 1 vial, 30 mL | 2-8°C, 6 months |
| Substrate Reagent A | 1 vial, 5 mL | 2-8℃ (Protect from light) |
| Substrate Reagent B | 1 vial, 5 mL | 2-8℃ (Protect from light) |
| Plate Sealer | 5 pieces | |
| Manual | 1 copy | |
| Certificate of Analysis | 1 copy | |
| Sample Type | Range (%) | Average Recovery (%) |
| Serum(n=8) | 89-102 | 95 |
| EDTA plasma(n=8) | 89-103 | 95 |
| Cell culture media(n=8) | 87-99 | 92 |
| | Intra-assay Precision | Intra-assay Precision | Intra-assay Precision | Inter-assay Precision | Inter-assay Precision | Inter-assay Precision |
| Sample | 1.00 | 2.00 | 3.00 | 1.00 | 2.00 | 3.00 |
| n | 20.00 | 20.00 | 20.00 | 20.00 | 20.00 | 20.00 |
| Mean (pg/mL) | 442.82 | 1252.08 | 3842.61 | 413.70 | 1166.91 | 3760.41 |
| Standard deviation | 36.84 | 98.29 | 410.39 | 51.71 | 108.76 | 399.73 |
| CV (%) | 8.32 | 7.85 | 10.68 | 12.50 | 9.32 | 10.63 |
12 months for antibodies. 6 months for ELISA Kits. Please see website T&Cs for further guidance
