| Applications: |
CLIA |
| Reactivity: |
Rat |
| Note: |
FOR SCIENTIFIC EDUCATIONAL RESEARCH USE ONLY (RUO). MUST NOT BE USED IN DIAGNOSTIC OR OTHER MEDICAL APPLICATIONS. |
| Sensitivity: |
75pg/mL |
| Detection Limit: |
125~8000pg/mL |
| Short Description: |
This rat F12 kit is a highly sensitive in-vitro chemiluminescent immunoassay for the measurement of trace amounts of analytes. |
| Storage Instruction: |
If unopened the kit may be stored at 2-8°C for up to 1 month. If the kit will not be used within 1 month, store the components separately, according to the component table in the manual. |
| Assay Time: |
3.5h |
| Detection: |
Chemiluminescence |
| Gene Symbol: |
F12 |
| Gene ID: |
306761 |
| Uniprot ID: |
FA12_RAT |
| Specificity: |
This kit recognizes Rat F12 in samples. No significant cross-reactivity or interference between Rat F12 and analogues was observed. |
| Sample Type: |
Serum, plasma and other biological fluids |
| Post Translational Modifications | O- and N-glycosylated. |
| Function | Factor XII is a serum glycoprotein that participates in the initiation of blood coagulation, fibrinolysis, and the generation of bradykinin and angiotensin. Prekallikrein is cleaved by factor XII to form kallikrein, which then cleaves factor XII first to alpha-factor XIIa and then trypsin cleaves it to beta-factor XIIa. Alpha-factor XIIa activates factor XI to factor XIa. |
| Protein Name | Coagulation Factor XiiHageman FactorHaf Cleaved Into - Coagulation Factor Xiia Heavy Chain - Coagulation Factor Xiia Light Chain |
| Database Links | Reactome: R-RNO-140837 |
| Cellular Localisation | Secreted |
| Alternative CLIA Names | Coagulation Factor Xii CLIA kitHageman Factor CLIA kitHaf Cleaved Into - Coagulation Factor Xiia Heavy Chain - Coagulation Factor Xiia Light Chain CLIA kitF12 CLIA kit |
| Specificity | This kit recognizes Rat F12 in samples. No significant cross-reactivity or interference between Rat F12 and analogues was observed. |
| Reproducibility | Both intra-CV and inter-CV are |
Information sourced from Uniprot.org
| Item | Specifications | Storage |
| Micro CLIA Plate (Dismountable) | 96T: 8 wells ×12 strips strips | -20℃, 6 months |
| Reference Standard | 96T: 2 vials 48T: 1 vial | -20℃, 6 months |
| Concentrated Biotinylated Detection Ab (100×) | 96T: 1 vial, 120 μL 60 μL | -20℃, 6 months |
| Concentrated HRP Conjugate (100×) | 96T: 1 vial, 120 μL 60 μL | -20℃ (Protect from light), 6 months |
| Reference Standard & Sample Diluent | 1 vial, 20 mL | 2-8°C, 6 months |
| Biotinylated Detection Ab Diluent | 1 vial, 14 mL | 2-8°C, 6 months |
| HRP Conjugate Diluent | 1 vial, 14 mL | 2-8°C, 6 months |
| Concentrated Wash Buffer (25×) | 1 vial, 30 mL | 2-8°C, 6 months |
| Substrate Reagent A | 1 vial, 5 mL | 2-8℃ (Protect from light) |
| Substrate Reagent B | 1 vial, 5 mL | 2-8℃ (Protect from light) |
| Plate Sealer | 5 pieces | |
| Manual | 1 copy | |
| Certificate of Analysis | 1 copy | |
| Sample Type | Range (%) | Average Recovery (%) |
| Serum(n=8) | 93-107 | 98 |
| EDTA plasma(n=8) | 95-110 | 101 |
| Cell culture media(n=8) | 89-102 | 95 |
| | Intra-assay Precision | Intra-assay Precision | Intra-assay Precision | Inter-assay Precision | Inter-assay Precision | Inter-assay Precision |
| Sample | 1.00 | 2.00 | 3.00 | 1.00 | 2.00 | 3.00 |
| n | 20.00 | 20.00 | 20.00 | 20.00 | 20.00 | 20.00 |
| Mean (pg/mL) | 437.47 | 685.58 | 3146.69 | 422.80 | 721.59 | 3416.12 |
| Standard deviation | 46.11 | 63.96 | 305.86 | 39.15 | 54.91 | 312.23 |
| CV (%) | 10.54 | 9.33 | 9.72 | 9.26 | 7.61 | 9.14 |
12 months for antibodies. 6 months for ELISA Kits. Please see website T&Cs for further guidance
