| Applications: |
ELISA |
| Reactivity: |
Rat |
| Note: |
STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS. |
| Sensitivity: |
12.5pg/mL |
| Detection Limit: |
31.2-2000pg/mL |
| Short Description: |
This ACAT1 Sandwich ELISA Kit is an in-vitro enzyme-linked immunosorbent assay for the measurement of samples in rat cell culture supernatant, serum and plasma (EDTA, citrate, heparin). |
| Storage Instruction: |
Store the unopened kit in the fridge at 2-8°C for up to 6 months. Once opened store individual kit contents according to components table provided with the kit. |
| Assay Time: |
4.5 hrs |
| Gene Symbol: |
Acat1 |
| Gene ID: |
25014 |
| Uniprot ID: |
THIL_RAT |
| Sample Type: |
tissue homogenates, cell lysates or other biological fluids. |
| Tissue Specificity | |
| Post Translational Modifications | Succinylation at Lys-265, adjacent to a coenzyme A binding site. Desuccinylated by SIRT5. |
| Function | This is one of the enzymes that catalyzes the last step of the mitochondrial beta-oxidation pathway, an aerobic process breaking down fatty acids into acetyl-CoA. Using free coenzyme A/CoA, catalyzes the thiolytic cleavage of medium- to long-chain 3-oxoacyl-CoAs into acetyl-CoA and a fatty acyl-CoA shortened by two carbon atoms. The activity of the enzyme is reversible and it can also catalyze the condensation of two acetyl-CoA molecules into acetoacetyl-CoA. Thereby, it plays a major role in ketone body metabolism. |
| Protein Name | Acetyl-Coa Acetyltransferase - MitochondrialAcetoacetyl-Coa Thiolase |
| Database Links | Reactome: R-RNO-70895Reactome: -RNO-77108Reactome: -RNO-77111 |
| Cellular Localisation | Mitochondrion |
| Alternative ELISA Names | Acetyl-Coa Acetyltransferase - Mitochondrial ELISA kitAcetoacetyl-Coa Thiolase ELISA kitAcat1 ELISA kit |
| output | |
Information sourced from Uniprot.org
12 months for antibodies. 6 months for ELISA Kits. Please see website T&Cs for further guidance
