Western Blot Antibodies | St John's Laboratory

HEK293 lysate (10ug protein in RIPA buffer) overexpressing Human CSF1R with C-terminal MYC tag probed with STJ72332 (0. 5ug/ml) in Lane A and probed with anti-MYC Tag (1/1000) in lane C. Mock-transfected HEK293 probed with STJ72332 (1mg/ml) in Lane B. Primary incubations were for 1 hour. Detected by chemiluminescence.

Anti-CSF1R antibody (700-710) (STJ72332)

Clonality :

Polyclonal

Host :

Goat

Applications :

Pep-ELISA/WB-Trf

Reactivity :

Human/Mouse/Rat/Dog/Cow

£312.50

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HEK293 lysate (10ug protein in RIPA buffer) overexpressing Human CSF1R with C-terminal MYC tag probed with STJ72333 (1ug/ml) in Lane A and probed with anti-MYC Tag (1/1000) in lane C. Mock-transfected HEK293 probed with STJ72333 (1mg/ml) in Lane B. Primary incubations were for 1 hour. Detected by chemiluminescence.

Anti-CSF1R antibody (734-744) (STJ72333)

Clonality :

Polyclonal

Host :

Goat

Applications :

Pep-ELISA/WB/IHC

Reactivity :

Human/Dog/Cow

£312.50

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Western blot analysis of various cells using c-Fms Polyclonal Antibody

Anti-CSF1R antibody (781-830 aa) (STJ92246)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/IHC/IF/ELISA

Reactivity :

Human/Rat/Mouse

£48.00 - £252.00

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Anti-CSF1R antibody (873-972) (STJ23236)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/IF/ICC/ELISA

Reactivity :

Human/Mouse/Rat

£112.50 - £524.50

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Western blot analysis using GM-CSF monoclonal antibody against CSF2-hIgGFc transfected HEK293 cell lysate.

Anti-CSF2 antibody (18-144 aa) [3D3] (STJ98113)

Clonality :

Monoclonal

Host :

Mouse

Applications :

WB/ELISA

Reactivity :

Human

£144.00 - £348.00

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Western blot analysis of HuT 78, using Human GM-CSF Rabbit polyclonal antibody (STJ11104714) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 180s.

Anti-CSF2 antibody (18-144) (STJ11104714)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/ELISA

Reactivity :

Human

£112.50 - £524.50

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Immunofluorescence analysis of U-2 OS cells using CSF2 antibody (STJ27880) at dilution of 1:100. Blue: DAPI for nuclear staining.

Anti-CSF2 antibody (20-36) (STJ27880)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/IF/ICC/ELISA

Reactivity :

Human/Mouse/Rat

£112.50 - £524.50

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Western blot analysis of L929 cells using CD116 Polyclonal Antibody.. Secondary antibody was diluted at 1:20000

Anti-CSF2RA antibody (121-170 aa) (STJ96627)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/IHC/IF/ELISA

Reactivity :

Human/Rat/Mouse

£48.00 - £252.00

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Western blot analysis of various lysates, using CD116/GM-CSFR Alpha antibody (STJ11104972) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Enhanced Kit. Exposure time: 180s.

Anti-CSF2RA antibody (23-320 aa) [S4972RM] (STJ11104972)

Clonality :

Monoclonal

Host :

Rabbit

Applications :

WB/ELISA

Reactivity :

Human

£129.50 - £574.50

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Western blot analysis of various lysates, using CSF2RB Rabbit polyclonal antibody (STJ112525) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Enhanced Kit. Exposure time: 30s.

Anti-CSF2RB antibody (200-443) (STJ112525)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/ELISA

Reactivity :

Human/Mouse

£112.50 - £524.50

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Western blot analysis of various cells using IL-3R Beta Polyclonal Antibody

Anti-CSF2RB antibody (559-608 aa) (STJ93698)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/IHC/IF/ELISA

Reactivity :

Human/Mouse/Rat

£48.00 - £252.00

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Anti-CSF3 antibody (30-207) (STJ118106)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/ELISA

Reactivity :

Human/Mouse/Rat

£112.50 - £524.50

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Immunohistochemistry analysis of paraffin-embedded mouse spleen using CSF3 antibody (STJ27939) at dilution of 1:100 (40x lens). Perform microwave antigen retrieval with 10 mM PBS buffer pH 7. 2 before commencing with immunohistochemistry staining protocol.

Anti-CSF3 antibody (30-207) (STJ27939)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/IHC-P/ELISA

Reactivity :

Human/Mouse/Rat

£112.50 - £524.50

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Western blot analysis of SKOV3 cells using G-CSFR Polyclonal Antibody.. Secondary antibody was diluted at 1:20000

Anti-CSF3R antibody (321-370 aa) (STJ96892)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/ELISA

Reactivity :

Human/Rat/Mouse

£48.00 - £252.00

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Anti-CSF3R antibody (650-750 aa) (STJ11106244)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/ELISA

Reactivity :

Human/Mouse/Rat

£112.50 - £524.50

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Western blot analysis of extracts of various cell lines, using CSF3R antibody (STJ29974) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 90s.

Anti-CSF3R antibody (650-750) (STJ29974)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/ELISA

Reactivity :

Human

£112.50 - £524.50

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Immunohistochemistry analysis of paraffin-embedded human breast carcinoma tissue, using CSGALNACT1 Antibody. The picture on the right is blocked with the synthesized peptide.

Anti-CSGALNACT1 antibody (201-250 aa) (STJ96371)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/IHC/IF/ELISA

Reactivity :

Human/Mouse/Rat

£48.00 - £252.00

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Western blot analysis of mouse-brain cells using Antibody diluted at 800. Secondary antibody was diluted at 1:20000

Anti-CSH1 antibody (161-210 aa) (STJ98652)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/IHC/IF/ELISA

Reactivity :

Human/Rat/Mouse

£48.00 - £252.00

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Western blot analysis of various lysates using Placental lactogen (CSH1) Rabbit pAb (STJ116739) at 1:3000 dilution. <br/>Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. <br/>Lysates/proteins: 25 Mu g per lane. <br/>Blocking buffer: 3% nonfat dry milk in TBST. <br/>Detection: ECL Basic Kit <br/>Exposure time: 90s.

Anti-CSH1 antibody (27-207) (STJ116739)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/ELISA

Reactivity :

Human

£112.50 - £524.50

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Anti-CSHL1 antibody (STJA0008660)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB

Reactivity :

Human

£48.00 - £252.00

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Immunohistochemistry analysis of paraffin-embedded mouse spleen using CSK Rabbit monoclonal antibody (STJ11101252) at dilution of 1:100 (40x lens). Perform microwave antigen retrieval with 10 mM Tris/EDTA buffer pH 9. 0 before commencing with immunohistochemistry staining protocol.

Anti-CSK antibody (1-100) [S2MR] (STJ11101252)

Clonality :

Monoclonal

Host :

Rabbit

Applications :

WB/IHC-P/ELISA

Reactivity :

Human/Mouse/Rat

£129.50 - £574.50

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Western blot analysis of various lysates using CSK Rabbit polyclonal antibody (STJ23240) at 1:1000 dilution. Secondary antibody:HRP Goat Anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/ proteins: 25 Mu g per lane. Blocking buffer: 3 % nonfat dry milk in TBST. Detection:ECL Enhanced Kit. Exposuretime: 90s.

Anti-CSK antibody (1-200) (STJ23240)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/ELISA

Reactivity :

Human/Mouse/Rat

£112.50 - £524.50

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Western blot analysis of extracts of various cell lines, using CSK rabbit polyclonal antibody (STJ11102784) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% non-fat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 90s.

Anti-CSK antibody (188-450) (STJ11102784)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/IHC-P/IF/ICC/ELISA

Reactivity :

Human/Mouse/Rat

£112.50 - £524.50

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Western blot analysis of Jurkat cells using Csk Polyclonal Antibody

Anti-CSK antibody (31-80 aa) (STJ92497)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/IF/ELISA

Reactivity :

Human/Mouse/Rat

£48.00 - £252.00

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Western blot analysis of various cells using Csk Polyclonal Antibody diluted at 1：1000

Anti-CSK antibody (330-379 aa) (STJ92496)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/IHC/IF/ELISA

Reactivity :

Human/Mouse/Rat

£48.00 - £252.00

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STJ70044 (0. 1µg/ml) staining of Jurkat lysate (35µg protein in RIPA buffer). Detected by chemiluminescence.

Anti-CSK antibody (C-Term) (STJ70044)

Clonality :

Polyclonal

Host :

Goat

Applications :

Pep-ELISA/WB

Reactivity :

Human/Mouse/Rat/Dog/Cow

£312.50

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Western blot detection of CSK in C6 and Jurkat cell lysates using CSK mouse mAb (1:1000 diluted).Predicted band size:50KDa.Observed band size:50KDa.

Anti-CSK antibody [2B4-C6-A10] (STJ99130)

Clonality :

Monoclonal

Host :

Mouse

Applications :

WB/ICC

Reactivity :

Human/Rat

£144.00 - £348.00

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Western blot analysis using Csk monoclonal antibody against NIH/3T3 (1) , HeLa (2) , COS7 (3) , Jurkat (4) , Raw246.7 (5) , A549 (6) , HL-60 (7) and PC-12 (8) cell lysate.

Anti-CSK antibody [5F3] (STJ97972)

Clonality :

Monoclonal

Host :

Mouse

Applications :

WB/IF/FC/ELISA

Reactivity :

Human/Mouse/Rat/Monkey

£144.00 - £348.00

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Anti-Csk antibody [CSK-04] (STJ16100298)

Clonality :

Monoclonal

Host :

Mouse

Applications :

WB/IP/ICC

Reactivity :

Human/Mouse

£537.50

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Anti-Csk binding protein antibody [PAG-C1] (STJ16100205)

Clonality :

Monoclonal

Host :

Mouse

Applications :

IP/WB/IHC-P

Reactivity :

Rat/Cow/Human/Mouse

£537.50

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Biotinylated STJ70044 (0. 5µg/ml) staining of Jurkat lysate (35µg protein in RIPA buffer) , exactly mirroring its parental non-biotinylated product. Primary incubation was 1 hour. Detected by chemiluminescence, using streptavidin-HRP and using NAP blocker as a substitute for skimmed milk.

Anti-CSK, Biotinylated antibody (C-Term) {Biotin} (STJ73187)

Clonality :

Polyclonal

Host :

Goat

Applications :

Pep-ELISA/WB

Reactivity :

Human/Mouse/Rat/Dog/Cow

£312.50

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Anti-Csk-binding protein antibody (STJ16101301)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

IP/WB/IHC-P

Reactivity :

Mouse/Human

£537.50

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Anti-CSMD1 antibody (5-55 aa) (STJ197991)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB

Reactivity :

Human/Mouse

£48.00 - £252.00

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Western blot analysis of various lysates using CSMD2 Rabbit polyclonal antibody (STJ116443) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 90s.

Anti-CSMD2 antibody (2050-2150) (STJ116443)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/ELISA

Reactivity :

Human/Mouse/Rat

£112.50 - £524.50

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Anti-CSMD3 antibody (1623-1673) (STJA0006434)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB

Reactivity :

Human/Mouse

£48.00 - £252.00

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Western blot analysis of various lysates using CSMD3 Rabbit polyclonal antibody (STJ114091) at 1:3000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 90s.

Anti-CSMD3 antibody (220-440) (STJ114091)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/ELISA

Reactivity :

Human

£112.50 - £524.50

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Anti-CSN1S1 antibody (16-135 aa) (STJ11107451)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/ELISA

Reactivity :

Mouse

£112.50 - £524.50

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Anti-CSN1S1 antibody (45-95 aa) (STJ197835)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB

Reactivity :

Human/Mouse/Rat

£48.00 - £252.00

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Western blot analysis of lysates from MCF7 cells, using CSN2 Rabbit polyclonal antibody (STJ118024) at 1:3000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 90s.

Anti-CSN2 antibody (1-100) (STJ118024)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/ELISA

Reactivity :

Human

£112.50 - £524.50

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Anti-CSN2 antibody (19-69) (STJA0006311)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB

Reactivity :

Human/Mouse/Rat

£48.00 - £252.00

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STJ70367 staining (0. 03µg/ml) of NIH3T3 lysates (RIPA buffer, 30µg total protein per lane). Primary incubated for 1 hour. Detected by chemiluminescence.

Anti-CSN2/TRIP15 antibody (C-Term) (STJ70367)

Clonality :

Polyclonal

Host :

Goat

Applications :

Pep-ELISA/WB/IHC

Reactivity :

Human/Mouse/Rat/Dog/Cow

£312.50

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Anti-CSN3 antibody (79-129 aa) (STJ197649)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB

Reactivity :

Human/Mouse/Rat

£48.00 - £252.00

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Western blot analysis of various cells using Casein Kinase I Alpha Polyclonal Antibody

Anti-CSNK1A1 antibody (126-175 aa) (STJ92010)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/IHC/IF/ELISA

Reactivity :

Human/Mouse/Rat

£48.00 - £252.00

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Western blot analysis of various lysates using Casein Kinase 1 alpha (CSNK1A1) Rabbit mAb (STJ11102475) at 1:1000 dilution. <br/>Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. <br/>Lysates/proteins: 25 Mu g per lane. <br/>Blocking buffer: 3% nonfat dry milk in TBST. <br/>Detection: ECL Basic Kit <br/>Exposure time: 1s.

Anti-CSNK1A1 antibody (238-337) [S5MR] (STJ11102475)

Clonality :

Monoclonal

Host :

Rabbit

Applications :

WB/IHC-P/IF/ICC/ELISA

Reactivity :

Human/Mouse/Rat

£129.50 - £574.50

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Immunohistochemistry analysis of Casein Kinase 1 alpha (CSNK1A1) in paraffin-embedded human placenta using Casein Kinase 1 alpha (CSNK1A1) Rabbit polyclonal antibody (STJ118665) at dilution of 1:100 (40x lens). Perform microwave antigen retrieval with 10 mM PBS buffer pH 7. 2 before commencing with immunohistochemistry staining protocol.

Anti-CSNK1A1 antibody (250-337) (STJ118665)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/IHC-P/IF/ICC/ELISA

Reactivity :

Human/Mouse/Rat

£112.50 - £524.50

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Western blot analysis using Casein Kinase I Alpha monoclonal antibody against truncated CK1 recombinant protein.

Anti-CSNK1A1 antibody [3C10F7/3C10G5/PMC01/4D12B3/3C10F7C11] (STJ97894)

Clonality :

Monoclonal

Host :

Mouse

Applications :

WB/IHC/IF/ELISA

Reactivity :

Human

£144.00 - £348.00

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Anti-CSNK1A1L antibody (1-337) (STJ28573)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/ELISA

Reactivity :

Human

£112.50 - £524.50

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Western blot analysis of brain cells using Casein Kinase I Delta Polyclonal Antibody

Anti-CSNK1D antibody (291-340 aa) (STJ92015)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/ELISA

Reactivity :

Human/Mouse/Rat

£48.00 - £252.00

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