Western Blot Antibodies | St John's Laboratory

Western blot analysis of K562 cells using CAF-1 p150 Polyclonal Antibody. Secondary antibody was diluted at 1:20000 cells nucleus extracted by Minute TM Cytoplasmic and Nuclear Fractionation kit (SC-003, Inventbiotech, MN, USA).

Anti-CHAF1A antibody (300-380 aa) (STJ96382)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/ELISA

Reactivity :

Human/Mouse

£48.00 - £252.00

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Immunohistochemistry analysis of paraffin-embedded mouse spleen using p60 CAF1 antibody (STJ28015) at dilution of 1:100 (40x lens). Perform microwave antigen retrieval with 10 mM PBS buffer pH 7. 2 before commencing with immunohistochemistry staining protocol.

Anti-CHAF1B antibody (270-559) (STJ28015)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/IHC-P/ELISA

Reactivity :

Human/Mouse/Rat

£112.50 - £524.50

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Immunohistochemistry analysis of paraffin-embedded human colon carcinoma using p60 CAF1 Rabbit monoclonal antibody (STJ11102241) at dilution of 1:100 (40x lens). Perform microwave antigen retrieval with 10 mM PBS buffer pH 7. 2 before commencing with immunohistochemistry staining protocol.

Anti-CHAF1B antibody (459-559) [S1MR] (STJ11102241)

Clonality :

Monoclonal

Host :

Rabbit

Applications :

WB/IHC-P/ELISA

Reactivity :

Human

£129.50 - £574.50

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Immunohistochemical analysis of paraffin-embedded Human brain. Antibody was diluted at 1:100 (4°C overnight). High-pressure and temperature Tris-EDTA, pH8.0 was used for antigen retrieval. Negetive contrl (right) obtaned from antibody was pre-absorbed by immunogen peptide.

Anti-CHAF1B antibody (71-120 aa) (STJ91970)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/IHC/IF/ELISA

Reactivity :

Human/Mouse/Rat

£48.00 - £252.00

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Western blot analysis of extracts of various tissues from the japonica rice (Oryza sativa L. ) variety Zhonghua 11, using Chalk5 Rabbit polyclonal antibody (STJ11104067) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Enhanced Kit. Exposure time: 3s.

Anti-Chalk5 antibody (1-140 aa) (STJ11104067)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/ELISA

Reactivity :

O.sativa

£146.50 - £642.50

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Immunofluorescence analysis of Hela cells using CHAMP1 Rabbit polyclonal antibody (STJ113290) at dilution of 1:100 (40x lens). Secondary antibody: Cy3 Goat Anti-Rabbit IgG (H+L) at 1:500 dilution. Blue: DAPI for nuclear staining.

Anti-CHAMP1 antibody (563-812) (STJ113290)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/IF/ICC/ELISA

Reactivity :

Human/Mouse

£112.50 - £524.50

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Anti-GroEL antibody at 1:2500 dilution; 50-100 µg of total protein per lane; rabbit polyclonal to goat IgG (HRP) at 1:10000 dilution

Anti-Chaperonin GroEL protein antibody (400aa C-Term) (STJ140039)

Clonality :

Polyclonal

Host :

Goat

Applications :

WB

Reactivity :

Bacteria

£101.50 - £394.50

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Immunohistochemistry analysis of CHAT in paraffin-embedded rat brain using CHAT Rabbit polyclonal antibody (STJ23113) at dilution of 1:50 (40x lens). Perform high pressure antigen retrieval with 10 mM citrate buffer pH 6. 0 before commencing with immunohistochemistry staining protocol.

Anti-CHAT antibody (200-300) (STJ23113)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/IHC-P/ELISA

Reactivity :

Human/Mouse/Rat

£112.50 - £524.50

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Immunofluorescence analysis of THP-1 cells using CHAT antibody (STJ115210) at dilution of 1:100. Blue: DAPI for nuclear staining.

Anti-CHAT antibody (331-630) (STJ115210)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/IHC-P/IF/ICC/ELISA

Reactivity :

Human/Mouse/Rat

£112.50 - £524.50

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Western blot analysis of various cells using Choactase Polyclonal Antibody diluted at 1：1000

Anti-CHAT antibody (334-383 aa) (STJ92274)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/ELISA

Reactivity :

Human/Mouse/Rat

£48.00 - £252.00

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Immunocytochemistry analysis of Choline Acetyltransferase (green) in SH-SY5Y using Choline Acetyltransferase antibody, and DAPI (blue).

Anti-CHAT antibody (735-748 aa) [R07-1G8] (STJA0011140)

Clonality :

Monoclonal

Host :

Rabbit

Applications :

WB/ICC/IF/IP

Reactivity :

Mouse/Rat

£264.50

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Anti-ChAT antibody (STJ13100065)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

IHC/WB

Reactivity :

Mouse/Rat/Human

£331.50

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Anti-ChAT antibody (STJ13100069)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

IHC/WB

Reactivity :

Rat/Mouse

£331.50

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Anti-ChAT antibody (STJ13100112)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

IHC/WB

Reactivity :

Rat/Mouse/Marmoset/Human

£331.50

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Anti-ChAT antibody (STJ13100500)

Clonality :

Polyclonal

Host :

Guinea Pig

Applications :

IHC/WB

Reactivity :

Rat/Mouse/Marmoset

£331.50

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Anti-ChAT antibody (STJ13100501)

Clonality :

Polyclonal

Host :

Guinea Pig

Applications :

IHC/WB

Reactivity :

Mouse

£331.50

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Anti-ChAT antibody (STJ13100790)

Clonality :

Polyclonal

Host :

Sheep

Applications :

IHC/WB

Reactivity :

Rat/Mouse/Human/Marmoset

£331.50

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Anti-ChAT antibody (STJ13100791)

Clonality :

Polyclonal

Host :

Sheep

Applications :

IHC/WB

Reactivity :

Rat/Mouse/Human/Marmoset

£331.50

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Western blot of rat hippocampal lysate showing specific immunolabeling of the ~70 kDa ChAT.

Anti-ChAT-Choline Acetyltransferase antibody (STJA0003837)

Clonality :

Polyclonal

Host :

Goat

Applications :

WB/IHC

Reactivity :

Chicken/Guinea Pig/Human/Mouse/Non-Human Primates/Rat

£454.50

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STJ70927 (1µg/ml) staining of Mouse (A) and Rat (B) Brain lysate (35µg protein in RIPA buffer). Detected by chemiluminescence.

Anti-Chat-Mouse antibody (C-Term) (STJ70927)

Clonality :

Polyclonal

Host :

Goat

Applications :

Pep-ELISA/WB/IHC

Reactivity :

Mouse/Rat

£312.50

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Western blot analysis of various lysates using CHCHD1 Rabbit polyclonal antibody (STJ118386) at 1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 90s.

Anti-CHCHD1 antibody (1-118) (STJ118386)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/ELISA

Reactivity :

Human/Mouse

£112.50 - £524.50

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Anti-CHCHD1 antibody (N-Term) (STJA0010348)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

IHC/WB

Reactivity :

Human/Mouse

£48.00 - £252.00

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Anti-CHCHD10 antibody (40-100 aa) (STJ11106347)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/ELISA

Reactivity :

Human

£112.50 - £524.50

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Anti-CHCHD10 antibody (STJA0006888)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB

Reactivity :

Human

£48.00 - £252.00

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Western blot analysis of extracts of various cell lines, using CHCHD2 antibody (STJ119068) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% non-fat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 90s.

Anti-CHCHD2 antibody (75-145) (STJ119068)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/IHC-P/IF/ICC/ELISA

Reactivity :

Human/Mouse/Rat

£129.50 - £642.50

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Anti-CHCHD2 antibody (STJA0006772)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB

Reactivity :

Human/Mouse

£48.00 - £252.00

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Western blot analysis of lysates from wild type (WT) and CHCHD3 knockout (KO) 293T cells, using [KO Validated] CHCHD3 Rabbit polyclonal antibody (STJ11100835) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 30s.

Anti-CHCHD3 antibody (1-227) (STJ11100835)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/ELISA

Reactivity :

Human/Mouse/Rat

£129.50 - £642.50

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Western blot analysis of various lysates using CHCHD3 Rabbit pAb (STJ111317) at 1:1000 dilution. <br/>Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. <br/>Lysates/proteins: 25 Mu g per lane. <br/>Blocking buffer: 3% nonfat dry milk in TBST. <br/>Detection: ECL Basic Kit <br/>Exposure time: 40s.

Anti-CHCHD3 antibody (1-227) (STJ111317)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/IF/ICC/IP/ELISA

Reactivity :

Human/Mouse/Rat

£112.50 - £524.50

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Anti-CHCHD3 antibody (144-194) (STJA0006536)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB

Reactivity :

Human/Mouse

£48.00 - £252.00

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STJ72149 (0. 3µg/ml) staining of Human Placenta lysate (35µg protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.

Anti-CHCHD3 antibody (151-164) (STJ72149)

Clonality :

Polyclonal

Host :

Goat

Applications :

Pep-ELISA/WB/IHC

Reactivity :

Human/Mouse/Rat/Dog/Cow/Sheep

£312.50

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STJ72148 (1µg/ml) staining of Human Heart lysate (35µg protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.

Anti-CHCHD3 antibody (Internal) (STJ72148)

Clonality :

Polyclonal

Host :

Goat

Applications :

Pep-ELISA/WB/IHC

Reactivity :

Human/Mouse/Rat/Dog/Cow/Sheep

£312.50

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Immunohistochemistry analysis of CHCHD4 in paraffin-embedded human liver cancer tissue using CHCHD4 Rabbit polyclonal antibody (STJ11105536) at a dilution of 1:100 (40x lens). High pressure antigen retrieval was performed with 0. 01 M citrate buffer (pH 6. 0) prior to immunohistochemistry staining.

Anti-CHCHD4 antibody (1-105) (STJ11105536)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/IHC-P/ELISA

Reactivity :

Human/Rat

£112.50 - £524.50

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Western blot analysis of various lysates using CHCHD4 Rabbit polyclonal antibody (STJ115105) at 1:3000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Enhanced Kit. Exposure time: 90s.

Anti-CHCHD4 antibody (1-142) (STJ115105)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/IHC-P/ELISA

Reactivity :

Human/Mouse/Rat

£112.50 - £524.50

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Western blot analysis of lysates from VEC cells, primary antibody was diluted at 1:1000, 4°C over night

Anti-CHCHD4 antibody (35-85 aa) (STJ193463)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB

Reactivity :

Human/Mouse/Rat

£48.00 - £252.00

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Anti-CHCHD5 antibody (STJA0009743)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB

Reactivity :

Human/Mouse

£48.00 - £252.00

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Western blot analysis of various lysates using CHCHD6 Rabbit polyclonal antibody (STJ114777) at 1:3000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 90s.

Anti-CHCHD6 antibody (1-235) (STJ114777)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/ELISA

Reactivity :

Human

£112.50 - £524.50

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Anti-CHCHD6 antibody (STJA0006997)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB

Reactivity :

Human/Mouse/Rat

£48.00 - £252.00

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Immunofluorescence analysis of L929 cells using CHCHD7 Rabbit polyclonal antibody (STJ11100443) at dilution of 1:100. Secondary antibody: Cy3 Goat Anti-Rabbit IgG (H+L) at 1:500 dilution. Blue: DAPI for nuclear staining.

Anti-CHCHD7 antibody (1-97) (STJ11100443)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/IF/ICC/ELISA

Reactivity :

Human/Mouse/Rat

£112.50 - £524.50

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Anti-CHCHD7 antibody (STJA0009445)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB

Reactivity :

Human/Mouse

£48.00 - £252.00

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Western blot analysis of lysates from HpeG2 cells, primary antibody was diluted at 1:1000, 4°C over night

Anti-CHCT1 antibody (108-158 aa) (STJ194037)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB

Reactivity :

Human/Mouse

£48.00 - £252.00

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Immunohistochemistry analysis of paraffin-embedded mouse colon using CHD1 Rabbit monoclonal antibody (STJ11105059) at dilution of 1:100 (40x lens). Perform high pressure antigen retrieval with 10 mM citrate buffer pH 6. 0 before commencing with immunohistochemistry staining protocol.

Anti-CHD1 antibody (1510-1710 aa) [S5059RM] (STJ11105059)

Clonality :

Monoclonal

Host :

Rabbit

Applications :

WB/IHC-P/ELISA

Reactivity :

Human/Mouse

£129.50 - £574.50

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Anti-CHD1 antibody (960-1040 aa) (STJ190179)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/ELISA

Reactivity :

Human/Mouse

£48.00 - £252.00

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Western blot analysis of lysates from wild type (WT) and CHD1 knockout (KO) 293T cells, using [KO Validated] CHD1 Rabbit pAb (STJ110193) at 1:500 dilution. <br/>Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. <br/>Lysates/proteins: 25 Mu g per lane. <br/>Blocking buffer: 3% nonfat dry milk in TBST. <br/>Detection: ECL Basic Kit <br/>Exposure time: 90s.

Anti-CHD1 antibody (C-Term) (STJ110193)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/IHC-P/ELISA

Reactivity :

Human/Mouse/Rat

£129.50 - £642.50

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Western blot analysis of extracts from NIH-3T3, Jurkat, A549 cells, using CHD1L Polyclonal Antibody.. Secondary antibody was diluted at 1:20000 cells nucleus extracted by Minute TM Cytoplasmic and Nuclear Fractionation kit (SC-003, Inventbiotech, MN, USA).

Anti-CHD1L antibody (545-594 aa) (STJ96392)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/ELISA

Reactivity :

Human/Mouse

£48.00 - £252.00

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Western blot analysis of extracts of HepG2 cells, using CHD1L Rabbit monoclonal antibody (STJ11102913) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 30s.

Anti-CHD1L antibody (700-800) [S3MR] (STJ11102913)

Clonality :

Monoclonal

Host :

Rabbit

Applications :

WB/ELISA

Reactivity :

Human

£129.50 - £574.50

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Western blot analysis of extracts of various cell lines, using CHD2 antibody (STJ28168) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 30s.

Anti-CHD2 antibody (1-223) (STJ28168)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/ELISA

Reactivity :

Human/Mouse/Rat

£112.50 - £524.50

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Western blot analysis of extracts of rat brain, using CHD2 antibody (STJ115438) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Enhanced Kit. Exposure time: 90s.

Anti-CHD2 antibody (1729-1828) (STJ115438)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/ELISA

Reactivity :

Rat

£112.50 - £524.50

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Western blot analysis of extracts of various cell lines, using CHD2 antibody (STJ115439) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 90s.

Anti-CHD2 antibody (1729-1828) (STJ115439)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/ELISA

Reactivity :

Human

£112.50 - £524.50

Choose Options