Immunofluorescence Antibodies | St John's Laboratory

Western blot analysis of various lysates using Hsp90 Alpha Rabbit pAb (STJ114322) at 1:1000 dilution. <br/>Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. <br/>Lysates/proteins: 25 Mu g per lane. <br/>Blocking buffer: 3% nonfat dry milk in TBST. <br/>Detection: ECL Basic Kit <br/>Exposure time: 30s.

Anti-HSP90AA1 antibody (629-732) (STJ114322)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/IHC-P/IF/ICC/ELISA

Reactivity :

Human/Mouse/Rat

£112.50 - £524.50

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Western blot analysis of 1) Hela, 2) Mouse Brain Tissue, 3) Rat Brain Tissue with HSP90 a Mouse mAb diluted at 1:2, 000.

Anti-HSP90AA1 antibody (660-740 aa) [1F6] (STJ97496)

Clonality :

Monoclonal

Host :

Mouse

Applications :

WB/IHC/IF

Reactivity :

Human/Rat/Mouse

£48.00 - £252.00

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Immunofluorescence analysis of Hela cell. 1, HSP90A Polyclonal Antibody (red) was diluted at 1:200 (4°C overnight). Active Caspase-3 monoclonal antibody (5E1) (green) was diluted at 1:200 (4°C overnight). 2, Goat Anti Rabbit Alexa Fluor 594 Catalog: (NA was diluted at 1:1000 (room temperature, 50min). Goat Anti Mouse Alexa Fluor 488 Catalog: (NA was diluted at 1:1000 (room temperature, 50min).

Anti-HSP90AA1 antibody (683-732 aa) (STJ93622)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/IHC/IF/ELISA

Reactivity :

Human/Mouse/Rat

£48.00 - £252.00

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Immunofluorescence analysis of U2OS cells using Hsp90 Beta Rabbit polyclonal antibody (STJ11104548). Secondary antibody: Cy3 Goat Anti-Rabbit IgG (H+L) at 1:500 dilution. Blue: DAPI for nuclear staining.

Anti-HSP90AB1 antibody (1-300) (STJ11104548)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/IHC-P/IF/ICC/ELISA

Reactivity :

Human/Mouse/Rat

£112.50 - £524.50

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Western blot analysis of lysates from wild type (WT) and HSP90AB1 knockout (KO) HeLa cells, using [KO Validated] Hsp90 Beta Rabbit polyclonal antibody (STJ119988) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 90s.

Anti-HSP90AB1 antibody (1-300) (STJ119988)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/IF/ICC/ELISA

Reactivity :

Human/Mouse/Rat/Monkey

£129.50 - £642.50

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Western blot analysis of various cells using HSP90 Beta Polyclonal Antibody diluted at 1：2000

Anti-HSP90AB1 antibody (226-275 aa) (STJ93609)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/IHC/IP/IF/ELISA

Reactivity :

Human/Mouse/Rat/Monkey

£48.00 - £252.00

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Western blot analysis using HSP90 Beta monoclonal antibody against Jurkat (1) , A431 (2) , HeLa (3) , A549 (4) , HEK293 (5) , K562 (6) , NIH/3T3 (7) , PC-12 (8) and Cos7 (9) cell lysate.

Anti-HSP90AB1 antibody [5G4] (STJ98144)

Clonality :

Monoclonal

Host :

Mouse

Applications :

WB/IHC/IF/FC/ELISA

Reactivity :

Human/Mouse/Rat/Monkey

£144.00 - £348.00

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Immunofluorescence analysis of paraffin-embedded Mouse colon cancer tissue using Grp94 Rabbit polyclonal antibody (STJ24090) at a dilution of 1:100 (40x lens). Secondary antibody:Cy3 Goat Anti-Rabbit IgG (H+L) at 1:500 dilution. Blue: DAPI for nuclear staining. Perform high pressure antigen retrieval with 0. 01 M citrate buffer (pH 6. 0) prior to IF staining.

Anti-HSP90B1 antibody (40-310) (STJ24090)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/IHC-P/IF/ICC/ELISA

Reactivity :

Human/Mouse/Rat

£112.50 - £524.50

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Immunofluorescence analysis of NIH-3T3 cells using Grp94 Polyclonal Antibody (STJ28194) at dilution of 1:100 (40x lens). Blue: DAPI for nuclear staining.

Anti-HSP90B1 antibody (501-750) (STJ28194)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/IHC-P/IF/ICC/ELISA

Reactivity :

Human/Mouse/Rat

£112.50 - £524.50

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Immunofluorescence analysis of PC-12 cells using Grp94 Rabbit polyclonal antibody (STJ11104452) at dilution of 1:100 (40x lens). Secondary antibody: Cy3 Goat Anti-Rabbit IgG (H+L) at 1:500 dilution. Blue: DAPI for nuclear staining.

Anti-HSP90B1 antibody (600-700) (STJ11104452)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/IF/ICC/ELISA

Reactivity :

Human/Mouse/Rat

£112.50 - £524.50

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Western blot analysis of various cells using Endoplasmin Polyclonal Antibody diluted at 1：1000

Anti-HSP90B1 antibody (754-803 aa) (STJ92925)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/IHC/IF/ELISA

Reactivity :

Human/Mouse

£48.00 - £252.00

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Western blot analysis of lysates from U-87MG cells, using HSPA13 Rabbit pAb (STJ116234) at 1:3000 dilution. <br/>Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. <br/>Lysates/proteins: 25 Mu g per lane. <br/>Blocking buffer: 3% nonfat dry milk in TBST. <br/>Detection: ECL Enhanced Kit <br/>Exposure time: 90s.

Anti-HSPA13 antibody (165-471) (STJ116234)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/IF/ICC/ELISA

Reactivity :

Human/Mouse/Rat

£112.50 - £524.50

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Confocal imaging of NIH/3T3 cells using HSPA14 Rabbit monoclonal antibody (STJ11101806, dilution 1:100) followed by a further incubation with Cy3 Goat Anti-Rabbit IgG (H+L) ( dilution 1:500) (Red). The cells were counterstained with Alpha-Tubulin Mouse monoclonal antibody ( dilution 1:400) followed by incubation with ABflo® 488-conjugated Goat Anti-Mouse IgG (H+L) antibody ( dilution 1:500) (Green). DAPI was used for nuclear staining (Blue). Objective: 100x.

Anti-HSPA14 antibody (1-100) [S6MR] (STJ11101806)

Clonality :

Monoclonal

Host :

Rabbit

Applications :

WB/IHC-P/IF/ICC/ELISA

Reactivity :

Human/Mouse

£129.50 - £574.50

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Immunocytochemistry analysis of HspA14 (green) in Jurkat using HspA14 antibody, and DAPI (blue).

Anti-HSPA14 antibody (75-89 aa) [R02-1I2] (STJA0012419)

Clonality :

Monoclonal

Host :

Rabbit

Applications :

WB/ICC/IF/IP

Reactivity :

Human/Mouse

£264.50

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Immunofluorescence analysis of U2OS cells using HSP70 Rabbit polyclonal antibody (STJ114814) at dilution of 1:100 (40x lens). Secondary antibody: Cy3 Goat Anti-Rabbit IgG (H+L) at 1:500 dilution. Blue: DAPI for nuclear staining.

Anti-HSPA1A antibody (500-600) (STJ114814)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/IHC-P/IF/ICC/ELISA

Reactivity :

Human/Mouse/Rat

£112.50 - £524.50

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Western blot analysis of extracts of various cell lines, using HSP70/HSPA1 antibody (STJ24091) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% non-fat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 10s.

Anti-HSPA1A antibody (500-600) (STJ24091)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/IHC-P/IF/ICC/ELISA

Reactivity :

Human/Mouse/Rat

£112.50 - £524.50

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Western blot analysis of various lysates using HSPA2 Rabbit pAb (STJ110211) at 1:1000 dilution. <br/>Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. <br/>Lysates/proteins: 25 Mu g per lane. <br/>Blocking buffer: 3% nonfat dry milk in TBST. <br/>Detection: ECL Basic Kit <br/>Exposure time: 60s.

Anti-HSPA2 antibody (400-639) (STJ110211)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/IF/ICC/ELISA

Reactivity :

Human/Mouse/Rat

£112.50 - £524.50

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Confocal imaging of paraffin-embedded Rat testis tissue using HSPA2 Rabbit monoclonal antibody (STJ11102321, dilution 1:100) followed by a further incubation with Cy3 Goat Anti-Rabbit IgG (H+L) ( dilution 1:500) (Red). DAPI was used for nuclear staining (Blue). Objective: 40x. Perform high pressure antigen retrieval with 0. 01 M citrate buffer (pH 6. 0) prior to IF staining.

Anti-HSPA2 antibody (488-637) [S1MR] (STJ11102321)

Clonality :

Monoclonal

Host :

Rabbit

Applications :

WB/IF/ICC/ELISA

Reactivity :

Human/Mouse/Rat

£129.50 - £574.50

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Western blot analysis using HSP70 monoclonal antibody against HeLa (1) cell lysate.

Anti-HSPA4 antibody [5A6] (STJ98143)

Clonality :

Monoclonal

Host :

Mouse

Applications :

WB/IHC/IF/ELISA

Reactivity :

Human

£144.00 - £348.00

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Immunofluorescence analysis of A431 cells using HSPA4L Rabbit polyclonal antibody (STJ119695) at dilution of 1:100. Secondary antibody: Cy3 Goat Anti-Rabbit IgG (H+L) at 1:500 dilution. Blue: DAPI for nuclear staining.

Anti-HSPA4L antibody (500-600) (STJ119695)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/IF/ICC/ELISA

Reactivity :

Human/Mouse/Rat

£112.50 - £524.50

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Western blot analysis of 3T3 Cell Lysate, antibody was diluted at 1:1000. Secondary antibody was diluted at 1:20000

Anti-HSPA5 antibody (505-570 aa) (STJ98969)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/IHC/IF/ELISA

Reactivity :

Human/Mouse/Rat/Fish

£48.00 - £252.00

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Immunofluorescence analysis of U2OS cells using BiP/GRP78 Rabbit polyclonal antibody (STJ113776) at dilution of 1:100 (40x lens). Blue: DAPI for nuclear staining.

Anti-HSPA5 antibody (550-654) (STJ113776)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/IHC-P/IF/ICC/ELISA

Reactivity :

Human/Mouse/Rat

£112.50 - £524.50

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Western blot analysis of 1) Hela, 2) Rat LiverTissue with GRP78/Bip Mouse mAb diluted at 1:2, 000.

Anti-HSPA5 antibody [10C9] (STJ97523)

Clonality :

Monoclonal

Host :

Mouse

Applications :

WB/IHC/IF

Reactivity :

Human/Rat

£48.00 - £252.00

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Western blot analysis of extracts from 3T3, A549, C6 and Hela cell lysates using BiP/GRP78 mouse mAb (1:1000 diluted).Predicted band size:72KDa.Observed band size:72KDa.

Anti-HSPA5 antibody [4F11-D5-C10] (STJ99068)

Clonality :

Monoclonal

Host :

Mouse

Applications :

WB/IF/IP

Reactivity :

Human/Mouse/Rat/Hamster

£144.00 - £348.00

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Anti-HSPA5 antibody [8G3] (STJ97526)

Clonality :

Monoclonal

Host :

Mouse

Applications :

WB/IHC/IF

Reactivity :

Human/Rat

£48.00 - £252.00

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STJ73249 (0. 1µg/ml) staining of HepG2 (A) , HeLa (B) and MCF7 (C) cell lysate (35µg protein in RIPA buffer). Detected by chemiluminescence.

Anti-HSPA5/GRP78 antibody (118-131) (STJ73249)

Clonality :

Polyclonal

Host :

Goat

Applications :

Pep-ELISA/IF/WB

Reactivity :

Human

£312.50

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STJ73250 (1µg/ml) staining of HepG2 (A) and MCF7 (B) lysates (35µg protein in RIPA buffer). Detected by chemiluminescence.

Anti-HSPA5/GRP78 antibody (Internal) (STJ73250)

Clonality :

Polyclonal

Host :

Goat

Applications :

Pep-ELISA/WB/IHC/IF

Reactivity :

Human

£312.50

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Immunofluorescence analysis of L929 cells using Hsc70/HSPA8 Rabbit polyclonal antibody (STJ24099) at dilution of 1:100. Blue: DAPI for nuclear staining.

Anti-HSPA8 antibody (510-615) (STJ24099)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/IHC-P/IF/ICC/ELISA

Reactivity :

Human/Mouse/Rat/Monkey

£112.50 - £524.50

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Western blot analysis of 1) Hela, 2) HepG2, 3) Raw, 4) Mouse Brain, 5) Rat Brain, 6) Rat Liver using HSC 70 Polyclonal Antibody. Secondary antibody (STJ99666) was diluted at 1:20000

Anti-HSPA8 antibody (588-638) (STJA0006703)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/IHC/IF

Reactivity :

Human/Mouse/Rat

£48.00 - £252.00

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Western blot analysis of 1) Hela, 2) Mouse Brain Tissue, 3) Rat Brain Tissue with HSC70 Mouse mAb diluted at 1:2, 000.

Anti-HSPA8 antibody (Internal) [6C7] (STJ97498)

Clonality :

Monoclonal

Host :

Mouse

Applications :

WB/IHC/IF

Reactivity :

Human/Rat/Mouse

£48.00 - £252.00

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STJ70949 (0. 03µg/ml) staining of HepG2 (A) and NIH3T3 (B) cell lysate (35µg protein in RIPA buffer). Detected by chemiluminescence.

Anti-HSPA8-Isoform 1 antibody (Internal) (STJ70949)

Clonality :

Polyclonal

Host :

Goat

Applications :

Pep-ELISA/WB/IF/IHC

Reactivity :

Human/Mouse/Rat/Dog

£312.50

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Immunofluorescence analysis of U2OS cells using Grp75/MOT/Grp75/MOT/HSPA9 antibody (STJ24100). Blue: DAPI for nuclear staining.

Anti-HSPA9 antibody (380-679) (STJ24100)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/IHC-P/IF/ICC/ELISA

Reactivity :

Human/Mouse/Rat

£112.50 - £524.50

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Immunofluorescence analysis of A549 cells using HSP27/HSP27/HSPB1 antibody (STJ115636). Blue: DAPI for nuclear staining.

Anti-HSPB1 antibody (1-100) (STJ115636)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/IF/ICC/ELISA

Reactivity :

Human

£112.50 - £524.50

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Immunoprecipitation analysis of 200 Mu g extracts of HepG2 cells, using 3 Mu g HSP27/HSP27/HSPB1 antibody (STJ11104354). Western blot was performed from the immunoprecipitate using HSP27/HSP27/HSPB1 antibody (STJ11104354) at a dilution of 1:1000.

Anti-HSPB1 antibody (1-120) (STJ11104354)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/IF/ICC/IP/ELISA

Reactivity :

Human/Mouse/Rat

£112.50 - £524.50

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Immunofluorescence analysis of HeLa cells using HSP27/HSP27/HSPB1 antibody (STJ118760) at dilution of 1:100. Blue: DAPI for nuclear staining.

Anti-HSPB1 antibody (1-120) (STJ118760)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/IF/ICC/ELISA

Reactivity :

Human/Mouse/Rat

£129.50 - £642.50

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Confocal imaging of NIH/3T3 cells using HSP27/HSPB1 Rabbit monoclonal antibody (STJ11101283, dilution 1:200) followed by a further incubation with Cy3 Goat Anti-Rabbit IgG (H+L) ( dilution 1:500) (Red). The cells were counterstained with Alpha-Tubulin Mouse monoclonal antibody ( dilution 1:400) followed by incubation with ABflo® 488-conjugated Goat Anti-Mouse IgG (H+L) antibody ( dilution 1:500) (Green). DAPI was used for nuclear staining (Blue). Objective: 100x.

Anti-HSPB1 antibody (100-205) [S3MR] (STJ11101283)

Clonality :

Monoclonal

Host :

Rabbit

Applications :

WB/IF/ICC/ELISA

Reactivity :

Human/Mouse/Rat

£146.50 - £642.50

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Immunofluorescence analysis of mouse-liver tissue. 1, HSP27 Polyclonal Antibody (red) was diluted at 1:200 (4°C, overnight). 2, Cy3 labled Secondary antibody was diluted at 1:300 (room temperature, 50min).3, Picture B: DAPI (blue) 10min. Picture A:Target. Picture B: DAPI. Picture C: merge of A+B

Anti-HSPB1 antibody (48-97 aa) (STJ93617)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

IF/WB/IHC/ELISA

Reactivity :

Human/Mouse/Rat

£48.00 - £252.00

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Western blot analysis using HSP27 monoclonal antibody against HeLa (1) , A549 (2) , Jurkat (3) , A431 (4) , HEK293 (5) , HepG2 (6) and PC-12 (7) cell lysate.

Anti-HSPB1 antibody [5D7] (STJ98141)

Clonality :

Monoclonal

Host :

Mouse

Applications :

WB/IHC/IF/FC/ELISA

Reactivity :

Human/Rat

£144.00 - £348.00

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Western blot analysis of Hela with HSP27 Mouse mAb diluted at 1:2, 000

Anti-HSPB1 antibody [Mix-mA] (STJ97485)

Clonality :

Monoclonal

Host :

Mouse

Applications :

WB/IHC/IF

Reactivity :

Human

£48.00 - £252.00

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Immunofluorescence analysis of L929 cells using HSPB11 Rabbit polyclonal antibody (STJ11100181) at dilution of 1:100. Secondary antibody: Cy3 Goat Anti-Rabbit IgG (H+L) at 1:500 dilution. Blue: DAPI for nuclear staining.

Anti-HSPB11 antibody (1-144) (STJ11100181)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/IHC-P/IF/ICC/ELISA

Reactivity :

Human/Mouse/Rat

£112.50 - £524.50

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Immunofluorescence analysis of U2OS cells using HSPB2 antibody (STJ26207). Blue: DAPI for nuclear staining.

Anti-HSPB2 antibody (1-182) (STJ26207)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/IF/ICC/ELISA

Reactivity :

Human/Mouse

£112.50 - £524.50

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Western blot analysis of various cells using HSP Beta 2 Polyclonal Antibody

Anti-HSPB2 antibody (121-170 aa) (STJ93612)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/IHC/IF/ELISA

Reactivity :

Human/Mouse/Rat

£48.00 - £252.00

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Immunohistochemistry analysis of paraffin-embedded human breast carcinoma tissue, using HSP20 Antibody. The picture on the right is blocked with the synthesized peptide.

Anti-HSPB6 antibody (5-54 aa) (STJ93615)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

IHC/IF/ELISA

Reactivity :

Human/Mouse/Rat

£48.00 - £252.00

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Western blot analysis of various lysates using HSP20/HSPB6 Rabbit mAb (STJ11102422) at 1:1000 dilution. <br/>Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. <br/>Lysates/proteins: 25 Mu g per lane. <br/>Blocking buffer: 3% nonfat dry milk in TBST. <br/>Detection: ECL Basic Kit <br/>Exposure time: 1s.

Anti-HSPB6 antibody (70-150) [S2MR] (STJ11102422)

Clonality :

Monoclonal

Host :

Rabbit

Applications :

WB/IHC-P/IF/ICC/ELISA

Reactivity :

Human/Mouse/Rat

£129.50 - £574.50

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Immunofluorescence analysis of U-2 OS cells using HSPB8/HSP22 Rabbit monoclonal antibody (STJ11101424) at dilution of 1:100 (40x lens). Blue: DAPI for nuclear staining.

Anti-HSPB8 antibody (1-100) [S4MR] (STJ11101424)

Clonality :

Monoclonal

Host :

Rabbit

Applications :

WB/IF/ICC/ELISA

Reactivity :

Human/Mouse/Rat

£129.50 - £574.50

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Immunofluorescence analysis of paraffin-embedded mouse heart using HSPB8/HSP22 Rabbit polyclonal antibody (STJ24102) at dilution of 1:100 (40x lens). Secondary antibody: Cy3 Goat Anti-Rabbit IgG (H+L) at 1:500 dilution. Blue: DAPI for nuclear staining.

Anti-HSPB8 antibody (1-196) (STJ24102)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/IHC-P/IF/ICC/ELISA

Reactivity :

Human/Mouse/Rat

£112.50 - £524.50

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Western blot analysis of HeLa cells using HSPBAP1 Polyclonal Antibody.. Secondary antibody was diluted at 1:20000

Anti-HSPBAP1 antibody (321-370 aa) (STJ96504)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/IHC/IF/ELISA

Reactivity :

Human/Rat/Mouse

£48.00 - £252.00

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STJ72588 (0. 3µg/ml) staining of HeLa lysate (35µg protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.

Anti-HSPC117 antibody (201-215) (STJ72588)

Clonality :

Polyclonal

Host :

Goat

Applications :

Pep-ELISA/WB/IHC/IF/FC

Reactivity :

Human/Mouse/Rat/Dog/Pig/Cow

£312.50

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