• Immunofluorescence analysis of PC-12 cells using Grp94 Rabbit polyclonal antibody (STJ11104452) at dilution of 1:100 (40x lens). Secondary antibody: Cy3 Goat Anti-Rabbit IgG (H+L) at 1:500 dilution. Blue: DAPI for nuclear staining.
  • Immunofluorescence analysis of NIH/3T3 cells using Grp94 Rabbit polyclonal antibody (STJ11104452) at dilution of 1:100 (40x lens). Secondary antibody: Cy3 Goat Anti-Rabbit IgG (H+L) at 1:500 dilution. Blue: DAPI for nuclear staining.
  • Immunofluorescence analysis of HeLa cells using Grp94 Rabbit polyclonal antibody (STJ11104452) at dilution of 1:100 (40x lens). Secondary antibody: Cy3 Goat Anti-Rabbit IgG (H+L) at 1:500 dilution. Blue: DAPI for nuclear staining.
  • Western blot analysis of various lysates using Grp94 Rabbit polyclonal antibody (STJ11104452) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Enhanced Kit. Exposure time: 90s.

Anti-HSP90B1 antibody (600-700) (STJ11104452)

SKU:
STJ11104452

£112.50 - £522.50
Current Stock:
Host: Rabbit
Applications: WB/IF
Reactivity: Human/Mouse/Rat
Note: STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS.
Short Description: Rabbit polyclonal antibody anti-Endoplasmin (600-700) is suitable for use in Western Blot and Immunofluorescence research applications.
Clonality: Polyclonal
Conjugation: Unconjugated
Isotype: IgG
Formulation: PBS with 0.02% Sodium Azide, 50% Glycerol, pH7.3.
Purification: Affinity purification
Dilution Range: WB 1:500-1:2000
IF/ICC 1:50-1:200
Storage Instruction: Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles.
Gene Symbol: HSP90B1
Gene ID: 7184
Uniprot ID: ENPL_HUMAN
Immunogen Region: 600-700
Immunogen: A synthetic peptide corresponding to a sequence within amino acids 600-700 of human Grp94 (NP_003290.1).
Immunogen Sequence: ESEKTKESREAVEKEFEPLL NWMKDKALKDKIEKAVVSQR LTESPCALVASQYGWSGNME RIMKAQAYQTGKDISTNYYA SQKKTFEINPRHPLIRDMLR R
Function Molecular chaperone that functions in the processing and transport of secreted proteins. When associated with CNPY3, required for proper folding of Toll-like receptors. Functions in endoplasmic reticulum associated degradation (ERAD). Has ATPase activity. May participate in the unfolding of cytosolic leaderless cargos (lacking the secretion signal sequence) such as the interleukin 1/IL-1 to facilitate their translocation into the ERGIC (endoplasmic reticulum-Golgi intermediate compartment) and secretion.the translocation process is mediated by the cargo receptor TMED10.
Protein Name Endoplasmin
94 Kda Glucose-Regulated Protein
Grp-94
Heat Shock Protein 90 Kda Beta Member 1
Tumor Rejection Antigen 1
Gp96 Homolog
Database Links Reactome: R-HSA-1679131
Reactome: R-HSA-3000480
Reactome: R-HSA-381183
Reactome: R-HSA-381426
Reactome: R-HSA-6785807
Reactome: R-HSA-8957275
Cellular Localisation Endoplasmic Reticulum Lumen
Sarcoplasmic Reticulum Lumen
Melanosome
Identified By Mass Spectrometry In Melanosome Fractions From Stage I To Stage Iv
Alternative Antibody Names Anti-Endoplasmin antibody
Anti-94 Kda Glucose-Regulated Protein antibody
Anti-Grp-94 antibody
Anti-Heat Shock Protein 90 Kda Beta Member 1 antibody
Anti-Tumor Rejection Antigen 1 antibody
Anti-Gp96 Homolog antibody
Anti-HSP90B1 antibody
Anti-GRP94 antibody
Anti-TRA1 antibody

Information sourced from Uniprot.org

12 months for antibodies. 6 months for ELISA Kits. Please see website T&Cs for further guidance