| Applications: |
ELISA |
| Reactivity: |
Mouse |
| Note: |
STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS. |
| Sensitivity: |
35pg/mL |
| Detection Limit: |
78.1-5000pg/mL |
| Short Description: |
This GPC1 Sandwich ELISA Kit is an in-vitro enzyme-linked immunosorbent assay for the measurement of samples in mouse cell culture supernatant, serum and plasma (EDTA, citrate, heparin). |
| Storage Instruction: |
Store the unopened kit in the fridge at 2-8°C for up to 6 months. Once opened store individual kit contents according to components table provided with the kit. |
| Assay Time: |
4.5 hrs |
| Gene Symbol: |
Gpc1 |
| Gene ID: |
14733 |
| Uniprot ID: |
GPC1_MOUSE |
| Sample Type: |
serum, plasma, tissue homogenates or other biological fluids. |
| Tissue Specificity | |
| Post Translational Modifications | S-nitrosylated in a Cu(2+)-dependent manner. Nitric acid (NO) is released from the nitrosylated cysteines by ascorbate or by some other reducing agent, in a Cu(2+) or Zn(2+) dependent manner. This free nitric oxide is then capable of cleaving the heparan sulfate side chains. N- and O-glycosylated. N-glycosylation is mainly of the complex type containing sialic acid. O-glycosylated with heparan sulfate. The heparan sulfate chains can be cleaved either by the action of heparanase or, degraded by a deaminative process that uses nitric oxide (NO) released from the S-nitrosylated cysteines. This process is triggered by ascorbate, or by some other reducing agent, in a Cu(2+)- or Zn(2+) dependent manner. Cu(2+) ions are provided by ceruloproteins such as APP, PRNP or CP which associate with GCP1 in intracellular compartments or lipid rafts. This cell-associated glypican is further processed to give rise to a medium-released species. |
| Function | Cell surface proteoglycan that bears heparan sulfate. Binds, via the heparan sulfate side chains, alpha-4 (V) collagen and participates in Schwann cell myelination. May act as a catalyst in increasing the rate of conversion of prion protein PRPN(C) to PRNP(Sc) via associating (via the heparan sulfate side chains) with both forms of PRPN, targeting them to lipid rafts and facilitating their interaction. Required for proper skeletal muscle differentiation by sequestering FGF2 in lipid rafts preventing its binding to receptors (FGFRs) and inhibiting the FGF-mediated signaling. Binds Cu(2+) or Zn(2+) ions. |
| Protein Name | Glypican-1 Cleaved Into - Secreted Glypican-1 |
| Database Links | Reactome: R-MMU-1971475Reactome: -MMU-2022928Reactome: -MMU-2024096Reactome: -MMU-202733Reactome: -MMU-376176Reactome: -MMU-975634 |
| Cellular Localisation | Cell MembraneLipid-AnchorGpi-AnchorExtracellular SideEndosomeS-Nitrosylated Form Recycled In EndosomesLocalizes To Cav1-Containing Vesicles Close To The Cell SurfaceCleavage Of Heparan Sulfate Side Chains Takes Place Mainly In Late EndosomesAssociates With Both Forms Of Prnp In Lipid RaftsColocalizes With App In Perinuclear Compartments And With Cp In Intracellular CompartmentsAssociates With Fibrillar App Amyloid-Beta Peptides In Lipid Rafts In Alzheimer Disease BrainsSecreted Glypican-1: SecretedExtracellular Space |
| Alternative ELISA Names | Glypican-1 Cleaved Into - Secreted Glypican-1 ELISA kitGpc1 ELISA kit |
| output | |
Information sourced from Uniprot.org
12 months for antibodies. 6 months for ELISA Kits. Please see website T&Cs for further guidance
