| Applications: |
ELISA |
| Reactivity: |
Human |
| Note: |
STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS. |
| Sensitivity: |
0.072ng/mL |
| Detection Limit: |
0.156-10ng/mL |
| Short Description: |
This UBE2L3 Sandwich ELISA Kit is an in-vitro enzyme-linked immunosorbent assay for the measurement of samples in human cell culture supernatant, serum and plasma (EDTA, citrate, heparin). |
| Storage Instruction: |
Store the unopened kit in the fridge at 2-8°C for up to 6 months. Once opened store individual kit contents according to components table provided with the kit. |
| Assay Time: |
4.5 hrs |
| Gene Symbol: |
UBE2L3 |
| Gene ID: |
7332 |
| Uniprot ID: |
UB2L3_HUMAN |
| Sample Type: |
tissue homogenates or other biological fluids. |
| Tissue Specificity | Ubiquitous, with highest expression in testis. |
| Post Translational Modifications | Ubiquitinated. The alteration of UBE2L3 protein levels during the S-phase of the cell cycle is due to ubiquitin-dependent proteasomal degradation. Autoubiquitinated in vitro. |
| Function | Ubiquitin-conjugating enzyme E2 that specifically acts with HECT-type and RBR family E3 ubiquitin-protein ligases. Does not function with most RING-containing E3 ubiquitin-protein ligases because it lacks intrinsic E3-independent reactivity with lysine: in contrast, it has activity with the RBR family E3 enzymes, such as PRKN, RNF31 and ARIH1, that function like RING-HECT hybrids. Accepts ubiquitin from the E1 complex and catalyzes its covalent attachment to other proteins. In vitro catalyzes 'Lys-11'-linked polyubiquitination. Involved in the selective degradation of short-lived and abnormal proteins. Down-regulated during the S-phase it is involved in progression through the cell cycle. Regulates nuclear hormone receptors transcriptional activity. May play a role in myelopoiesis. |
| Protein Name | Ubiquitin-Conjugating Enzyme E2 L3E2 Ubiquitin-Conjugating Enzyme L3L-UbcUbch7Ubiquitin Carrier Protein L3Ubiquitin-Conjugating Enzyme E2-F1Ubiquitin-Protein Ligase L3 |
| Database Links | Reactome: R-HSA-5357905Reactome: R-HSA-5675482Reactome: R-HSA-8866652Reactome: R-HSA-8866654Reactome: R-HSA-983168 |
| Cellular Localisation | NucleusCytoplasm |
| Alternative ELISA Names | Ubiquitin-Conjugating Enzyme E2 L3 ELISA kitE2 Ubiquitin-Conjugating Enzyme L3 ELISA kitL-Ubc ELISA kitUbch7 ELISA kitUbiquitin Carrier Protein L3 ELISA kitUbiquitin-Conjugating Enzyme E2-F1 ELISA kitUbiquitin-Protein Ligase L3 ELISA kitUBE2L3 ELISA kitUBCE7 ELISA kitUBCH7 ELISA kit |
| output | |
Information sourced from Uniprot.org
12 months for antibodies. 6 months for ELISA Kits. Please see website T&Cs for further guidance
