| Applications: |
ELISA |
| Reactivity: |
Human |
| Note: |
STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS. |
| Sensitivity: |
0.081ng/mL |
| Detection Limit: |
0.312-20ng/mL |
| Short Description: |
This UBE2A Sandwich ELISA Kit is an in-vitro enzyme-linked immunosorbent assay for the measurement of samples in human cell culture supernatant, serum and plasma (EDTA, citrate, heparin). |
| Storage Instruction: |
Store the unopened kit in the fridge at 2-8°C for up to 6 months. Once opened store individual kit contents according to components table provided with the kit. |
| Assay Time: |
4.5 hrs |
| Gene Symbol: |
UBE2A |
| Gene ID: |
7319 |
| Uniprot ID: |
UBE2A_HUMAN |
| Sample Type: |
serum, plasma, tissue homogenates, cell lysates, cell culture supernates or other biological fluids. |
| Tissue Specificity | |
| Post Translational Modifications | Phosphorylation at Ser-120 by CDK9 increases activity towards histone H2B. |
| Function | Accepts ubiquitin from the E1 complex and catalyzes its covalent attachment to other proteins. In association with the E3 enzyme BRE1 (RNF20 and/or RNF40), it plays a role in transcription regulation by catalyzing the monoubiquitination of histone H2B at 'Lys-120' to form H2BK120ub1. H2BK120ub1 gives a specific tag for epigenetic transcriptional activation, elongation by RNA polymerase II, telomeric silencing, and is also a prerequisite for H3K4me and H3K79me formation. In vitro catalyzes 'Lys-11', as well as 'Lys-48'-linked polyubiquitination. Required for postreplication repair of UV-damaged DNA. |
| Protein Name | Ubiquitin-Conjugating Enzyme E2 AE2 Ubiquitin-Conjugating Enzyme ARad6 Homolog AHr6aHhr6aUbiquitin Carrier Protein AUbiquitin-Protein Ligase A |
| Database Links | Reactome: R-HSA-8866652Reactome: R-HSA-8866654Reactome: R-HSA-983168 |
| Cellular Localisation | |
| Alternative ELISA Names | Ubiquitin-Conjugating Enzyme E2 A ELISA kitE2 Ubiquitin-Conjugating Enzyme A ELISA kitRad6 Homolog A ELISA kitHr6a ELISA kitHhr6a ELISA kitUbiquitin Carrier Protein A ELISA kitUbiquitin-Protein Ligase A ELISA kitUBE2A ELISA kitRAD6A ELISA kit |
| output | |
Information sourced from Uniprot.org
12 months for antibodies. 6 months for ELISA Kits. Please see website T&Cs for further guidance
