| Applications: |
ELISA |
| Reactivity: |
Human |
| Note: |
FOR SCIENTIFIC EDUCATIONAL RESEARCH USE ONLY (RUO). MUST NOT BE USED IN DIAGNOSTIC OR OTHER MEDICAL APPLICATIONS. |
| Sensitivity: |
0.19ng/mL |
| Detection Limit: |
0.31~20ng/mL |
| Short Description: |
This TXN Sandwich ELISA is an in-vitro enzyme-linked immunosorbent assay for the measurement of samples in human cell culture supernatant, serum and plasma (EDTA, citrate, heparin). |
| Storage Instruction: |
If unopened the kit may be stored at 2-8°C for up to 1 month. If the kit will not be used within 1 month, store the components separately, according to the component table in the manual. |
| Assay Time: |
3.5h |
| Detection: |
Colormetric |
| Gene Symbol: |
TXN |
| Gene ID: |
7295 |
| Uniprot ID: |
THIO_HUMAN |
| Specificity: |
This kit recognizes Human Trx in samples. No significant cross-reactivity or interference between Human Trx and analogues was observed. |
| Sample Type: |
Serum, plasma and other biological fluids |
| Function | Participates in various redox reactions through the reversible oxidation of its active center dithiol to a disulfide and catalyzes dithiol-disulfide exchange reactions. Plays a role in the reversible S-nitrosylation of cysteine residues in target proteins, and thereby contributes to the response to intracellular nitric oxide. Nitrosylates the active site Cys of CASP3 in response to nitric oxide (NO), and thereby inhibits caspase-3 activity. Induces the FOS/JUN AP-1 DNA-binding activity in ionizing radiation (IR) cells through its oxidation/reduction status and stimulates AP-1 transcriptional activity. ADF augments the expression of the interleukin-2 receptor TAC (IL2R/P55). |
| Protein Name | ThioredoxinTrxAtl-Derived FactorAdfSurface-Associated Sulphydryl ProteinSaspAllergen Hom S Trx |
| Database Links | Reactome: R-HSA-2559580Reactome: R-HSA-3299685Reactome: R-HSA-499943Reactome: R-HSA-5628897Reactome: R-HSA-5676934Reactome: R-HSA-844456Reactome: R-HSA-9617629Reactome: R-HSA-9660826 |
| Cellular Localisation | NucleusCytoplasmSecretedTranslocates From The Cytoplasm Into The Nucleus After Phorbol 12-Myristate 13-Acetate Induction (Pma)Predominantly In The Cytoplasm In Non Irradiated CellsRadiation Induces Translocation Of Trx From The Cytoplasm To The NucleusSecreted By A Leaderless Secretory Pathway |
| Alternative ELISA Names | Thioredoxin ELISA kitTrx ELISA kitAtl-Derived Factor ELISA kitAdf ELISA kitSurface-Associated Sulphydryl Protein ELISA kitSasp ELISA kitAllergen Hom S Trx ELISA kitTXN ELISA kitTRDX ELISA kitTRX ELISA kitTRX1 ELISA kit |
| Specificity | This kit recognizes Human Trx in samples. No significant cross-reactivity or interference between Human Trx and analogues was observed. |
| Reproducibility | Both intra-CV and inter-CV are |
Information sourced from Uniprot.org
| Item | Specifications | Storage |
| Micro ELISA Plate (Dismountable) | 96T: 8 wells ×12 strips strips | -20℃, 6 months |
| Reference Standard | 96T: 2 vials 48T: 1 vial | -20℃, 6 months |
| Concentrated Biotinylated Detection Ab (100×) | 96T: 1 vial, 120 μL 60 μL | -20℃, 6 months |
| Concentrated HRP Conjugate (100×) | 96T: 1 vial, 120 μL 60 μL | -20℃ (Protect from light), 6 months |
| Reference Standard & Sample Diluent | 1 vial, 20 mL | 2-8°C, 6 months |
| Biotinylated Detection Ab Diluent | 1 vial, 14 mL | 2-8°C, 6 months |
| HRP Conjugate Diluent | 1 vial, 14 mL | 2-8°C, 6 months |
| Concentrated Wash Buffer (25×) | 1 vial, 30 mL | 2-8°C, 6 months |
| Substrate Reagent | 1 vial, 10 mL | 2-8℃ (Protect from light) |
| Stop Solution | 1 vial, 10 mL | 2-8°C |
| Plate Sealer | 5 pieces | |
| Manual | 1 copy | |
| Certificate of Analysis | 1 copy | |
| Sample Type | Range (%) | Average Recovery (%) |
| Serum(n=8) | 84-95 | 90 |
| EDTA plasma (n=8) | 89-103 | 95 |
| Cell culture media (n=8) | 93-107 | 101 |
| | Intra-assay Precision | Intra-assay Precision | Intra-assay Precision | Inter-assay Precision | Inter-assay Precision | Inter-assay Precision |
| Sample | 1.00 | 2.00 | 3.00 | 1.00 | 2.00 | 3.00 |
| n | 20.00 | 20.00 | 20.00 | 20.00 | 20.00 | 20.00 |
| Mean (ng/mL) | 1.00 | 2.96 | 9.77 | 1.03 | 3.22 | 10.25 |
| Standard deviation | 0.05 | 0.14 | 0.33 | 0.07 | 0.17 | 0.46 |
| CV (%) | 5.00 | 4.73 | 3.38 | 6.80 | 5.28 | 4.49 |
12 months for antibodies. 6 months for ELISA Kits. Please see website T&Cs for further guidance
