| Applications: |
ELISA |
| Reactivity: |
Rat |
| Note: |
STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS. |
| Sensitivity: |
0.137ng/mL |
| Detection Limit: |
0.312-20ng/mL |
| Short Description: |
This Trx Sandwich ELISA Kit is an in-vitro enzyme-linked immunosorbent assay for the measurement of samples in rat cell culture supernatant, serum and plasma (EDTA, citrate, heparin). |
| Storage Instruction: |
Store the unopened kit in the fridge at 2-8°C for up to 6 months. Once opened store individual kit contents according to components table provided with the kit. |
| Assay Time: |
4.5 hrs |
| Gene Symbol: |
Txn |
| Gene ID: |
116484 |
| Uniprot ID: |
THIO_RAT |
| Sample Type: |
serum, plasma or other biological fluids. |
| Tissue Specificity | |
| Post Translational Modifications | In the fully reduced protein, both Cys-69 and Cys-73 are nitrosylated in response to nitric oxide (NO). When two disulfide bonds are present in the protein, only Cys-73 is nitrosylated. Cys-73 can serve as donor for nitrosylation of target proteins. |
| Function | Participates in various redox reactions through the reversible oxidation of its active center dithiol to a disulfide and catalyzes dithiol-disulfide exchange reactions. Plays a role in the reversible S-nitrosylation of cysteine residues in target proteins, and thereby contributes to the response to intracellular nitric oxide. Nitrosylates the active site Cys of CASP3 in response to nitric oxide (NO), and thereby inhibits caspase-3 activity. Induces the FOS/JUN AP-1 DNA binding activity in ionizing radiation (IR) cells through its oxidation/reduction status and stimulates AP-1 transcriptional activity. |
| Protein Name | ThioredoxinTrx |
| Database Links | Reactome: R-RNO-2559580Reactome: -RNO-3299685Reactome: -RNO-499943Reactome: -RNO-5628897Reactome: -RNO-5676934Reactome: -RNO-844456 |
| Cellular Localisation | NucleusCytoplasmSecretedTranslocates From The Cytoplasm Into The Nucleus After Phorbol 12-Myristate 13-Acetate Induction (Pma)Predominantly In The Cytoplasm In Non Irradiated CellsRadiation Induces Translocation Of Trx From The Cytoplasm To The NucleusSecreted By A Leaderless Secretory Pathway |
| Alternative ELISA Names | Thioredoxin ELISA kitTrx ELISA kitTxn ELISA kitTxn1 ELISA kit |
| output | |
Information sourced from Uniprot.org
12 months for antibodies. 6 months for ELISA Kits. Please see website T&Cs for further guidance
