| Applications: |
ELISA |
| Reactivity: |
Human |
| Note: |
FOR SCIENTIFIC EDUCATIONAL RESEARCH USE ONLY (RUO). MUST NOT BE USED IN DIAGNOSTIC OR OTHER MEDICAL APPLICATIONS. |
| Sensitivity: |
0.09ng/mL |
| Detection Limit: |
0.16~10ng/mL |
| Short Description: |
This TXNRD1 Sandwich ELISA is an in-vitro enzyme-linked immunosorbent assay for the measurement of samples in human cell culture supernatant, serum and plasma (EDTA, citrate, heparin). |
| Storage Instruction: |
If unopened the kit may be stored at 2-8°C for up to 1 month. If the kit will not be used within 1 month, store the components separately, according to the component table in the manual. |
| Assay Time: |
3.5h |
| Detection: |
Colormetric |
| Gene Symbol: |
TXNRD1 |
| Gene ID: |
7296 |
| Uniprot ID: |
TRXR1_HUMAN |
| Specificity: |
This kit recognizes Human TrxR1 in samples. No significant cross-reactivity or interference between Human TrxR1 and analogues was observed. |
| Sample Type: |
Serum, plasma and other biological fluids |
| Post Translational Modifications | Isoform 5: The N-terminus is blocked. ISGylated. |
| Function | Reduces disulfideprotein thioredoxin (Trx) to its dithiol-containing form. Homodimeric flavoprotein involved in the regulation of cellular redox reactions, growth and differentiation. Contains a selenocysteine residue at the C-terminal active site that is essential for catalysis (Probable). Also has reductase activity on hydrogen peroxide (H2O2). Isoform 1: Induces actin and tubulin polymerization, leading to formation of cell membrane protrusions. Isoform 4: Enhances the transcriptional activity of estrogen receptors ESR1 and ESR2. Isoform 5: Enhances the transcriptional activity of the estrogen receptor ESR2 only. Mediates cell death induced by a combination of interferon-beta and retinoic acid. |
| Protein Name | Thioredoxin Reductase 1 - CytoplasmicTrGene Associated With Retinoic And Interferon-Induced Mortality 12 ProteinGrim-12Gene Associated With Retinoic And Ifn-Induced Mortality 12 ProteinKm-102-Derived Reductase-Like FactorPeroxidase Txnrd1Thioredoxin Reductase Tr1 |
| Database Links | Reactome: R-HSA-1989781Reactome: R-HSA-2408550Reactome: R-HSA-3299685Reactome: R-HSA-499943Reactome: R-HSA-5263617Reactome: R-HSA-5336415Reactome: R-HSA-5628897Reactome: R-HSA-9818027 |
| Cellular Localisation | Isoform 1: CytoplasmIsoform 4: CytoplasmNucleusIsoform 5: Cytoplasm |
| Alternative ELISA Names | Thioredoxin Reductase 1 - Cytoplasmic ELISA kitTr ELISA kitGene Associated With Retinoic And Interferon-Induced Mortality 12 Protein ELISA kitGrim-12 ELISA kitGene Associated With Retinoic And Ifn-Induced Mortality 12 Protein ELISA kitKm-102-Derived Reductase-Like Factor ELISA kitPeroxidase Txnrd1 ELISA kitThioredoxin Reductase Tr1 ELISA kitTXNRD1 ELISA kitGRIM12 ELISA kitKDRF ELISA kit |
| Specificity | This kit recognizes Human TrxR1 in samples. No significant cross-reactivity or interference between Human TrxR1 and analogues was observed. |
| Reproducibility | Both intra-CV and inter-CV are |
Information sourced from Uniprot.org
| Item | Specifications | Storage |
| Micro ELISA Plate (Dismountable) | 96T: 8 wells ×12 strips strips | -20℃, 6 months |
| Reference Standard | 96T: 2 vials 48T: 1 vial | -20℃, 6 months |
| Concentrated Biotinylated Detection Ab (100×) | 96T: 1 vial, 120 μL 60 μL | -20℃, 6 months |
| Concentrated HRP Conjugate (100×) | 96T: 1 vial, 120 μL 60 μL | -20℃ (Protect from light), 6 months |
| Reference Standard & Sample Diluent | 1 vial, 20 mL | 2-8°C, 6 months |
| Biotinylated Detection Ab Diluent | 1 vial, 14 mL | 2-8°C, 6 months |
| HRP Conjugate Diluent | 1 vial, 14 mL | 2-8°C, 6 months |
| Concentrated Wash Buffer (25×) | 1 vial, 30 mL | 2-8°C, 6 months |
| Substrate Reagent | 1 vial, 10 mL | 2-8℃ (Protect from light) |
| Stop Solution | 1 vial, 10 mL | 2-8°C |
| Plate Sealer | 5 pieces | |
| Manual | 1 copy | |
| Certificate of Analysis | 1 copy | |
| Sample Type | Range (%) | Average Recovery (%) |
| Serum(n=8) | 86-98 | 93 |
| EDTA plasma (n=8) | 95-108 | 100 |
| Cell culture media (n=8) | 88-100 | 94 |
| | Intra-assay Precision | Intra-assay Precision | Intra-assay Precision | Inter-assay Precision | Inter-assay Precision | Inter-assay Precision |
| Sample | 1.00 | 2.00 | 3.00 | 1.00 | 2.00 | 3.00 |
| n | 20.00 | 20.00 | 20.00 | 20.00 | 20.00 | 20.00 |
| Mean (ng/mL) | 0.46 | 1.42 | 4.77 | 0.44 | 1.48 | 5.18 |
| Standard deviation | 0.02 | 0.06 | 0.23 | 0.03 | 0.09 | 0.21 |
| CV (%) | 4.35 | 4.23 | 4.82 | 6.82 | 6.08 | 4.05 |
12 months for antibodies. 6 months for ELISA Kits. Please see website T&Cs for further guidance
