| Applications: |
ELISA |
| Reactivity: |
Human |
| Note: |
FOR SCIENTIFIC EDUCATIONAL RESEARCH USE ONLY (RUO). MUST NOT BE USED IN DIAGNOSTIC OR OTHER MEDICAL APPLICATIONS. |
| Sensitivity: |
0.09ng/mL |
| Detection Limit: |
0.16~10ng/mL |
| Short Description: |
This TBP2 Sandwich ELISA is an in-vitro enzyme-linked immunosorbent assay for the measurement of samples in human cell culture supernatant, serum and plasma (EDTA, citrate, heparin). |
| Storage Instruction: |
If unopened the kit may be stored at 2-8°C for up to 1 month. If the kit will not be used within 1 month, store the components separately, according to the component table in the manual. |
| Assay Time: |
3.5h |
| Detection: |
Colormetric |
| Gene Symbol: |
TXNIP |
| Gene ID: |
10628 |
| Uniprot ID: |
TXNIP_HUMAN |
| Specificity: |
This kit recognizes Human TBP2 in samples. No significant cross-reactivity or interference between Human TBP2 and analogues was observed. |
| Sample Type: |
Serum, plasma and other biological fluids |
| Function | May act as an oxidative stress mediator by inhibiting thioredoxin activity or by limiting its bioavailability. Interacts with COPS5 and restores COPS5-induced suppression of CDKN1B stability, blocking the COPS5-mediated translocation of CDKN1B from the nucleus to the cytoplasm. Functions as a transcriptional repressor, possibly by acting as a bridge molecule between transcription factors and corepressor complexes, and over-expression will induce G0/G1 cell cycle arrest. Required for the maturation of natural killer cells. Acts as a suppressor of tumor cell growth. Inhibits the proteasomal degradation of DDIT4, and thereby contributes to the inhibition of the mammalian target of rapamycin complex 1 (mTORC1). |
| Protein Name | Thioredoxin-Interacting ProteinThioredoxin-Binding Protein 2Vitamin D3 Up-Regulated Protein 1 |
| Database Links | Reactome: R-HSA-844456Reactome: R-HSA-9617629Reactome: R-HSA-9660826Reactome: R-HSA-9707564 |
| Cellular Localisation | Cytoplasm |
| Alternative ELISA Names | Thioredoxin-Interacting Protein ELISA kitThioredoxin-Binding Protein 2 ELISA kitVitamin D3 Up-Regulated Protein 1 ELISA kitTXNIP ELISA kitVDUP1 ELISA kit |
| Specificity | This kit recognizes Human TBP2 in samples. No significant cross-reactivity or interference between Human TBP2 and analogues was observed. |
| Reproducibility | Both intra-CV and inter-CV are |
Information sourced from Uniprot.org
| Item | Specifications | Storage |
| Micro ELISA Plate (Dismountable) | 96T: 8 wells ×12 strips strips | -20℃, 6 months |
| Reference Standard | 96T: 2 vials 48T: 1 vial | -20℃, 6 months |
| Concentrated Biotinylated Detection Ab (100×) | 96T: 1 vial, 120 μL 60 μL | -20℃, 6 months |
| Concentrated HRP Conjugate (100×) | 96T: 1 vial, 120 μL 60 μL | -20℃ (Protect from light), 6 months |
| Reference Standard & Sample Diluent | 1 vial, 20 mL | 2-8°C, 6 months |
| Biotinylated Detection Ab Diluent | 1 vial, 14 mL | 2-8°C, 6 months |
| HRP Conjugate Diluent | 1 vial, 14 mL | 2-8°C, 6 months |
| Concentrated Wash Buffer (25×) | 1 vial, 30 mL | 2-8°C, 6 months |
| Substrate Reagent | 1 vial, 10 mL | 2-8℃ (Protect from light) |
| Stop Solution | 1 vial, 10 mL | 2-8°C |
| Plate Sealer | 5 pieces | |
| Manual | 1 copy | |
| Certificate of Analysis | 1 copy | |
| Sample Type | Range (%) | Average Recovery (%) |
| Serum(n=8) | 89-106 | 97 |
| EDTA plasma (n=8) | 94-111 | 101 |
| Cell culture media (n=8) | 89-102 | 95 |
| | Intra-assay Precision | Intra-assay Precision | Intra-assay Precision | Inter-assay Precision | Inter-assay Precision | Inter-assay Precision |
| Sample | 1.00 | 2.00 | 3.00 | 1.00 | 2.00 | 3.00 |
| n | 20.00 | 20.00 | 20.00 | 20.00 | 20.00 | 20.00 |
| Mean (ng/mL) | 0.46 | 1.15 | 4.20 | 0.44 | 1.13 | 3.90 |
| Standard deviation | 0.03 | 0.06 | 0.17 | 0.03 | 0.06 | 0.16 |
| CV (%) | 6.52 | 5.22 | 4.05 | 6.82 | 5.31 | 4.10 |
12 months for antibodies. 6 months for ELISA Kits. Please see website T&Cs for further guidance
