| Applications: |
ELISA |
| Reactivity: |
Human |
| Note: |
STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS. |
| Sensitivity: |
0.058ng/mL |
| Detection Limit: |
0.156-10ng/mL |
| Short Description: |
This SPTLC3 Sandwich ELISA Kit is an in-vitro enzyme-linked immunosorbent assay for the measurement of samples in human cell culture supernatant, serum and plasma (EDTA, citrate, heparin). |
| Storage Instruction: |
Store the unopened kit in the fridge at 2-8°C for up to 6 months. Once opened store individual kit contents according to components table provided with the kit. |
| Assay Time: |
4.5 hrs |
| Gene Symbol: |
SPTLC3 |
| Gene ID: |
55304 |
| Uniprot ID: |
SPTC3_HUMAN |
| Sample Type: |
tissue homogenates or other biological fluids. |
| Tissue Specificity | Expressed in most tissues, except peripheral blood cells and bone marrow, with highest levels in heart, kidney, liver, uterus and skin. |
| Post Translational Modifications | |
| Function | Component of the serine palmitoyltransferase multisubunit enzyme (SPT) that catalyzes the initial and rate-limiting step in sphingolipid biosynthesis by condensing L-serine and activated acyl-CoA (most commonly palmitoyl-CoA) to form long-chain bases. The SPT complex is composed of SPTLC1, SPTLC2 or SPTLC3 and SPTSSA or SPTSSB. Within this complex, the heterodimer consisting of SPTLC1 and SPTLC2/SPTLC3 forms the catalytic core. The composition of the serine palmitoyltransferase (SPT) complex determines the substrate preference. The SPTLC1-SPTLC2-SPTSSA complex shows a strong preference for C16-CoA substrate, while the SPTLC1-SPTLC3-SPTSSA isozyme uses both C14-CoA and C16-CoA as substrates, with a slight preference for C14-CoA. The SPTLC1-SPTLC2-SPTSSB complex shows a strong preference for C18-CoA substrate, while the SPTLC1-SPTLC3-SPTSSB isozyme displays an ability to use a broader range of acyl-CoAs, without apparent preference. |
| Protein Name | Serine Palmitoyltransferase 3Long Chain Base Biosynthesis Protein 2bLcb2bLong Chain Base Biosynthesis Protein 3Lcb 3Serine-Palmitoyl-Coa Transferase 3Spt 3 |
| Database Links | Reactome: R-HSA-1660661 |
| Cellular Localisation | Endoplasmic Reticulum MembraneSingle-Pass Membrane Protein |
| Alternative ELISA Names | Serine Palmitoyltransferase 3 ELISA kitLong Chain Base Biosynthesis Protein 2b ELISA kitLcb2b ELISA kitLong Chain Base Biosynthesis Protein 3 ELISA kitLcb 3 ELISA kitSerine-Palmitoyl-Coa Transferase 3 ELISA kitSpt 3 ELISA kitSPTLC3 ELISA kitC20orf38 ELISA kitSPTLC2L ELISA kit |
| output | |
Information sourced from Uniprot.org
12 months for antibodies. 6 months for ELISA Kits. Please see website T&Cs for further guidance
