• Human REPIN1 (Replication initiator 1) CLIA Kit (STJC0000779)

Human REPIN1 (Replication initiator 1) CLIA Kit (STJC0000779)

SKU:
STJC0000779

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Applications: CLIA
Reactivity: Human
Note: FOR SCIENTIFIC EDUCATIONAL RESEARCH USE ONLY (RUO). MUST NOT BE USED IN DIAGNOSTIC OR OTHER MEDICAL APPLICATIONS.
Sensitivity: 37.5pg/mL
Detection Limit: 62.5~4000pg/mL
Short Description: This human REPIN1 kit is a highly sensitive in-vitro chemiluminescent immunoassay for the measurement of trace amounts of analytes.
Storage Instruction: If unopened the kit may be stored at 2-8°C for up to 1 month. If the kit will not be used within 1 month, store the components separately, according to the component table in the manual.
Assay Time: 3.5h
Detection: Chemiluminescence
Gene Symbol: REPIN1
Gene ID: 29803
Uniprot ID: REPI1_HUMAN
Specificity: This kit recognizes Human REPIN1 in samples. No significant cross-reactivity or interference between Human REPIN1 and analogues was observed.
Sample Type: Serum, plasma and other biological fluids
Function Sequence-specific double-stranded DNA-binding protein required for initiation of chromosomal DNA replication. Binds on 5'-ATT-3' reiterated sequences downstream of the origin of bidirectional replication (OBR) and a second, homologous ATT sequence of opposite orientation situated within the OBR zone. Facilitates DNA bending.
Protein Name Replication Initiator 1
60 Kda Origin-Specific Dna-Binding Protein
60 Kda Replication Initiation Region Protein
Att-Binding Protein
Dhfr Oribeta-Binding Protein Rip60
Zinc Finger Protein 464
Cellular Localisation Nucleus
Alternative CLIA Names Replication Initiator 1 CLIA kit
60 Kda Origin-Specific Dna-Binding Protein CLIA kit
60 Kda Replication Initiation Region Protein CLIA kit
Att-Binding Protein CLIA kit
Dhfr Oribeta-Binding Protein Rip60 CLIA kit
Zinc Finger Protein 464 CLIA kit
REPIN1 CLIA kit
RIP60 CLIA kit
ZNF464 CLIA kit
Specificity This kit recognizes Human REPIN1 in samples. No significant cross-reactivity or interference between Human REPIN1 and analogues was observed.
Reproducibility Both intra-CV and inter-CV are

Information sourced from Uniprot.org


Item Specifications Storage
Micro CLIA Plate (Dismountable) 96T: 8 wells ×12 strips strips -20℃, 6 months
Reference Standard 96T: 2 vials 48T: 1 vial -20℃, 6 months
Concentrated Biotinylated Detection Ab (100×) 96T: 1 vial, 120 μL 60 μL -20℃, 6 months
Concentrated HRP Conjugate (100×) 96T: 1 vial, 120 μL 60 μL -20℃ (Protect from light), 6 months
Reference Standard & Sample Diluent 1 vial, 20 mL 2-8°C, 6 months
Biotinylated Detection Ab Diluent 1 vial, 14 mL 2-8°C, 6 months
HRP Conjugate Diluent 1 vial, 14 mL 2-8°C, 6 months
Concentrated Wash Buffer (25×) 1 vial, 30 mL 2-8°C, 6 months
Substrate Reagent A 1 vial, 5 mL 2-8℃ (Protect from light)
Substrate Reagent B 1 vial, 5 mL 2-8℃ (Protect from light)
Plate Sealer 5 pieces
Manual 1 copy
Certificate of Analysis 1 copy

Sample Type Range (%) Average Recovery (%)
Serum(n=8) 90-105 97
EDTA plasma(n=8) 93-107 100
Cell culture media(n=8) 96-109 102

Intra-assay Precision Intra-assay Precision Intra-assay Precision Inter-assay Precision Inter-assay Precision Inter-assay Precision
Sample 1.00 2.00 3.00 1.00 2.00 3.00
n 20.00 20.00 20.00 20.00 20.00 20.00
Mean (pg/mL) 203.73 473.54 1490.81 195.05 429.53 1583.76
Standard deviation 22.23 54.65 163.99 17.50 32.77 133.99
CV (%) 10.91 11.54 11.00 8.97 7.63 8.46