Applications: | CLIA |
Reactivity: | Human |
Note: | FOR SCIENTIFIC EDUCATIONAL RESEARCH USE ONLY (RUO). MUST NOT BE USED IN DIAGNOSTIC OR OTHER MEDICAL APPLICATIONS. |
Sensitivity: | 37.5pg/mL |
Detection Limit: | 62.5~4000pg/mL |
Short Description: | This human REPIN1 kit is a highly sensitive in-vitro chemiluminescent immunoassay for the measurement of trace amounts of analytes. |
Storage Instruction: | If unopened the kit may be stored at 2-8°C for up to 1 month. If the kit will not be used within 1 month, store the components separately, according to the component table in the manual. |
Assay Time: | 3.5h |
Detection: | Chemiluminescence |
Gene Symbol: | REPIN1 |
Gene ID: | 29803 |
Uniprot ID: | REPI1_HUMAN |
Specificity: | This kit recognizes Human REPIN1 in samples. No significant cross-reactivity or interference between Human REPIN1 and analogues was observed. |
Sample Type: | Serum, plasma and other biological fluids |
Function | Sequence-specific double-stranded DNA-binding protein required for initiation of chromosomal DNA replication. Binds on 5'-ATT-3' reiterated sequences downstream of the origin of bidirectional replication (OBR) and a second, homologous ATT sequence of opposite orientation situated within the OBR zone. Facilitates DNA bending. |
Protein Name | Replication Initiator 1 60 Kda Origin-Specific Dna-Binding Protein 60 Kda Replication Initiation Region Protein Att-Binding Protein Dhfr Oribeta-Binding Protein Rip60 Zinc Finger Protein 464 |
Cellular Localisation | Nucleus |
Alternative CLIA Names | Replication Initiator 1 CLIA kit 60 Kda Origin-Specific Dna-Binding Protein CLIA kit 60 Kda Replication Initiation Region Protein CLIA kit Att-Binding Protein CLIA kit Dhfr Oribeta-Binding Protein Rip60 CLIA kit Zinc Finger Protein 464 CLIA kit REPIN1 CLIA kit RIP60 CLIA kit ZNF464 CLIA kit |
Specificity | This kit recognizes Human REPIN1 in samples. No significant cross-reactivity or interference between Human REPIN1 and analogues was observed. |
Reproducibility | Both intra-CV and inter-CV are |
Information sourced from Uniprot.org
Item | Specifications | Storage |
---|---|---|
Micro CLIA Plate (Dismountable) | 96T: 8 wells ×12 strips strips | -20℃, 6 months |
Reference Standard | 96T: 2 vials 48T: 1 vial | -20℃, 6 months |
Concentrated Biotinylated Detection Ab (100×) | 96T: 1 vial, 120 μL 60 μL | -20℃, 6 months |
Concentrated HRP Conjugate (100×) | 96T: 1 vial, 120 μL 60 μL | -20℃ (Protect from light), 6 months |
Reference Standard & Sample Diluent | 1 vial, 20 mL | 2-8°C, 6 months |
Biotinylated Detection Ab Diluent | 1 vial, 14 mL | 2-8°C, 6 months |
HRP Conjugate Diluent | 1 vial, 14 mL | 2-8°C, 6 months |
Concentrated Wash Buffer (25×) | 1 vial, 30 mL | 2-8°C, 6 months |
Substrate Reagent A | 1 vial, 5 mL | 2-8℃ (Protect from light) |
Substrate Reagent B | 1 vial, 5 mL | 2-8℃ (Protect from light) |
Plate Sealer | 5 pieces | |
Manual | 1 copy | |
Certificate of Analysis | 1 copy |
Sample Type | Range (%) | Average Recovery (%) |
---|---|---|
Serum(n=8) | 90-105 | 97 |
EDTA plasma(n=8) | 93-107 | 100 |
Cell culture media(n=8) | 96-109 | 102 |
Intra-assay Precision | Intra-assay Precision | Intra-assay Precision | Inter-assay Precision | Inter-assay Precision | Inter-assay Precision | |
---|---|---|---|---|---|---|
Sample | 1.00 | 2.00 | 3.00 | 1.00 | 2.00 | 3.00 |
n | 20.00 | 20.00 | 20.00 | 20.00 | 20.00 | 20.00 |
Mean (pg/mL) | 203.73 | 473.54 | 1490.81 | 195.05 | 429.53 | 1583.76 |
Standard deviation | 22.23 | 54.65 | 163.99 | 17.50 | 32.77 | 133.99 |
CV (%) | 10.91 | 11.54 | 11.00 | 8.97 | 7.63 | 8.46 |