| Applications: |
CLIA |
| Reactivity: |
Human |
| Note: |
FOR SCIENTIFIC EDUCATIONAL RESEARCH USE ONLY (RUO). MUST NOT BE USED IN DIAGNOSTIC OR OTHER MEDICAL APPLICATIONS. |
| Sensitivity: |
37.5pg/mL |
| Detection Limit: |
62.5~4000pg/mL |
| Short Description: |
This human REPIN1 kit is a highly sensitive in-vitro chemiluminescent immunoassay for the measurement of trace amounts of analytes. |
| Storage Instruction: |
If unopened the kit may be stored at 2-8°C for up to 1 month. If the kit will not be used within 1 month, store the components separately, according to the component table in the manual. |
| Assay Time: |
3.5h |
| Detection: |
Chemiluminescence |
| Gene Symbol: |
REPIN1 |
| Gene ID: |
29803 |
| Uniprot ID: |
REPI1_HUMAN |
| Specificity: |
This kit recognizes Human REPIN1 in samples. No significant cross-reactivity or interference between Human REPIN1 and analogues was observed. |
| Sample Type: |
Serum, plasma and other biological fluids |
| Function | Sequence-specific double-stranded DNA-binding protein required for initiation of chromosomal DNA replication. Binds on 5'-ATT-3' reiterated sequences downstream of the origin of bidirectional replication (OBR) and a second, homologous ATT sequence of opposite orientation situated within the OBR zone. Facilitates DNA bending. |
| Protein Name | Replication Initiator 160 Kda Origin-Specific Dna-Binding Protein60 Kda Replication Initiation Region ProteinAtt-Binding ProteinDhfr Oribeta-Binding Protein Rip60Zinc Finger Protein 464 |
| Cellular Localisation | Nucleus |
| Alternative CLIA Names | Replication Initiator 1 CLIA kit60 Kda Origin-Specific Dna-Binding Protein CLIA kit60 Kda Replication Initiation Region Protein CLIA kitAtt-Binding Protein CLIA kitDhfr Oribeta-Binding Protein Rip60 CLIA kitZinc Finger Protein 464 CLIA kitREPIN1 CLIA kitRIP60 CLIA kitZNF464 CLIA kit |
| Specificity | This kit recognizes Human REPIN1 in samples. No significant cross-reactivity or interference between Human REPIN1 and analogues was observed. |
| Reproducibility | Both intra-CV and inter-CV are |
Information sourced from Uniprot.org
| Item | Specifications | Storage |
| Micro CLIA Plate (Dismountable) | 96T: 8 wells ×12 strips strips | -20℃, 6 months |
| Reference Standard | 96T: 2 vials 48T: 1 vial | -20℃, 6 months |
| Concentrated Biotinylated Detection Ab (100×) | 96T: 1 vial, 120 μL 60 μL | -20℃, 6 months |
| Concentrated HRP Conjugate (100×) | 96T: 1 vial, 120 μL 60 μL | -20℃ (Protect from light), 6 months |
| Reference Standard & Sample Diluent | 1 vial, 20 mL | 2-8°C, 6 months |
| Biotinylated Detection Ab Diluent | 1 vial, 14 mL | 2-8°C, 6 months |
| HRP Conjugate Diluent | 1 vial, 14 mL | 2-8°C, 6 months |
| Concentrated Wash Buffer (25×) | 1 vial, 30 mL | 2-8°C, 6 months |
| Substrate Reagent A | 1 vial, 5 mL | 2-8℃ (Protect from light) |
| Substrate Reagent B | 1 vial, 5 mL | 2-8℃ (Protect from light) |
| Plate Sealer | 5 pieces | |
| Manual | 1 copy | |
| Certificate of Analysis | 1 copy | |
| Sample Type | Range (%) | Average Recovery (%) |
| Serum(n=8) | 90-105 | 97 |
| EDTA plasma(n=8) | 93-107 | 100 |
| Cell culture media(n=8) | 96-109 | 102 |
| | Intra-assay Precision | Intra-assay Precision | Intra-assay Precision | Inter-assay Precision | Inter-assay Precision | Inter-assay Precision |
| Sample | 1.00 | 2.00 | 3.00 | 1.00 | 2.00 | 3.00 |
| n | 20.00 | 20.00 | 20.00 | 20.00 | 20.00 | 20.00 |
| Mean (pg/mL) | 203.73 | 473.54 | 1490.81 | 195.05 | 429.53 | 1583.76 |
| Standard deviation | 22.23 | 54.65 | 163.99 | 17.50 | 32.77 | 133.99 |
| CV (%) | 10.91 | 11.54 | 11.00 | 8.97 | 7.63 | 8.46 |
12 months for antibodies. 6 months for ELISA Kits. Please see website T&Cs for further guidance
