Host: | HEK293 cells |
Note: | STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS. |
Formulation: | Lyophilised from a 0.22 Mu m filtered solution of PBS, pH 7.4. |
Storage Instruction: | Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles. |
Determination Method: | < 0.01 EU/Mu g of the protein by LAL method. |
Immunoreactivity: | Measured by its ability to inhibit papain cleavage of a fluorogenic peptide substrate Z-FR-AMC. The IC50 value is approximately 16 nM. |
Gene Symbol: | CST6 |
Gene ID: | 1474 |
Uniprot ID: | CYTM_HUMAN |
Immunogen Region: | Arg29-Met149 |
Immunogen: | Recombinant Human Cystatin-M/CST6 Protein is produced by HEK293 expression system. The target protein is expressed with sequence (Arg29-Met149) of human CST6 (Accession #NP_001314.1) fused with a 6×His tag at the C-terminus. |
Immunogen Sequence: | RPQERMVGELRDLSPDDPQV QKAAQAAVASYNMGSNSIYY FRDTHIIKAQSQLVAGIKYF LTMEMGSTDCRKTRVTGDHV DLTTCPLAAGAQQEKLRCDF EVLVVPWQNSSQLLKHNCVQ M |
Post Translational Modifications | Substrate for transglutaminases. Acts as an acyl acceptor but not as an acyl donor. |
Function | High affinity inhibitor for cathepsin L, cathepsin L2 (cathepsin V), and legumain. Involved in the regulation of epidermal cornification, and hair follicle morphogenesis and maintenance. |
Protein Name | Cystatin-MCystatin-6Cystatin-E |
Cellular Localisation | Secreted |
Alternative Protein Names | Cystatin-M proteinCystatin-6 proteinCystatin-E proteinCST6 protein |
Information sourced from Uniprot.org