| Applications: |
CLIA |
| Reactivity: |
Human |
| Note: |
FOR SCIENTIFIC EDUCATIONAL RESEARCH USE ONLY (RUO). MUST NOT BE USED IN DIAGNOSTIC OR OTHER MEDICAL APPLICATIONS. |
| Sensitivity: |
7.5pg/mL |
| Detection Limit: |
12.5~800pg/mL |
| Short Description: |
This human CLCF1 kit is a highly sensitive in-vitro chemiluminescent immunoassay for the measurement of trace amounts of analytes. |
| Storage Instruction: |
If unopened the kit may be stored at 2-8°C for up to 1 month. If the kit will not be used within 1 month, store the components separately, according to the component table in the manual. |
| Assay Time: |
3.5h |
| Detection: |
Chemiluminescence |
| Gene Symbol: |
CLCF1 |
| Gene ID: |
23529 |
| Uniprot ID: |
CLCF1_HUMAN |
| Specificity: |
This kit recognizes Human CLCF1 in samples. No significant cross-reactivity or interference between Human CLCF1 and analogues was observed. |
| Sample Type: |
Serum, plasma and other biological fluids |
| Tissue Specificity | Expressed predominantly in lymph nodes, spleen, peripheral blood lymphocytes, bone marrow, and fetal liver. |
| Function | In complex with CRLF1, forms a heterodimeric neurotropic cytokine that plays a crucial role during neuronal development (Probable). Also stimulates B-cells. Binds to and activates the ILST/gp130 receptor. |
| Protein Name | Cardiotrophin-Like Cytokine Factor 1B-Cell-Stimulating Factor 3Bsf-3Novel Neurotrophin-1Nnt-1 |
| Database Links | Reactome: R-HSA-6788467 |
| Cellular Localisation | Secreted |
| Alternative CLIA Names | Cardiotrophin-Like Cytokine Factor 1 CLIA kitB-Cell-Stimulating Factor 3 CLIA kitBsf-3 CLIA kitNovel Neurotrophin-1 CLIA kitNnt-1 CLIA kitCLCF1 CLIA kitBSF3 CLIA kitCLC CLIA kitNNT1 CLIA kit |
| Specificity | This kit recognizes Human CLCF1 in samples. No significant cross-reactivity or interference between Human CLCF1 and analogues was observed. |
| Reproducibility | Both intra-CV and inter-CV are |
Information sourced from Uniprot.org
| Item | Specifications | Storage |
| Micro CLIA Plate (Dismountable) | 96T: 8 wells ×12 strips strips | -20℃, 6 months |
| Reference Standard | 96T: 2 vials 48T: 1 vial | -20℃, 6 months |
| Concentrated Biotinylated Detection Ab (100×) | 96T: 1 vial, 120 μL 60 μL | -20℃, 6 months |
| Concentrated HRP Conjugate (100×) | 96T: 1 vial, 120 μL 60 μL | -20℃ (Protect from light), 6 months |
| Reference Standard & Sample Diluent | 1 vial, 20 mL | 2-8°C, 6 months |
| Biotinylated Detection Ab Diluent | 1 vial, 14 mL | 2-8°C, 6 months |
| HRP Conjugate Diluent | 1 vial, 14 mL | 2-8°C, 6 months |
| Concentrated Wash Buffer (25×) | 1 vial, 30 mL | 2-8°C, 6 months |
| Substrate Reagent A | 1 vial, 5 mL | 2-8℃ (Protect from light) |
| Substrate Reagent B | 1 vial, 5 mL | 2-8℃ (Protect from light) |
| Plate Sealer | 5 pieces | |
| Manual | 1 copy | |
| Certificate of Analysis | 1 copy | |
| Sample Type | Range (%) | Average Recovery (%) |
| Serum(n=8) | 87-101 | 93 |
| EDTA plasma(n=8) | 94-110 | 102 |
| Cell culture media(n=8) | 92-108 | 100 |
| | Intra-assay Precision | Intra-assay Precision | Intra-assay Precision | Inter-assay Precision | Inter-assay Precision | Inter-assay Precision |
| Sample | 1.00 | 2.00 | 3.00 | 1.00 | 2.00 | 3.00 |
| n | 20.00 | 20.00 | 20.00 | 20.00 | 20.00 | 20.00 |
| Mean (pg/mL) | 39.47 | 92.73 | 303.04 | 37.21 | 100.65 | 324.43 |
| Standard deviation | 3.88 | 8.65 | 22.97 | 3.36 | 8.83 | 21.54 |
| CV (%) | 9.83 | 9.33 | 7.58 | 9.03 | 8.77 | 6.64 |
12 months for antibodies. 6 months for ELISA Kits. Please see website T&Cs for further guidance
