| Applications: |
ELISA |
| Reactivity: |
Human |
| Note: |
FOR SCIENTIFIC EDUCATIONAL RESEARCH USE ONLY (RUO). MUST NOT BE USED IN DIAGNOSTIC OR OTHER MEDICAL APPLICATIONS. |
| Sensitivity: |
75pg/mL |
| Detection Limit: |
125~8000pg/mL |
| Short Description: |
This AQP1 Sandwich ELISA is an in-vitro enzyme-linked immunosorbent assay for the measurement of samples in human cell culture supernatant, serum and plasma (EDTA, citrate, heparin). |
| Storage Instruction: |
If unopened the kit may be stored at 2-8°C for up to 1 month. If the kit will not be used within 1 month, store the components separately, according to the component table in the manual. |
| Assay Time: |
3.5h |
| Detection: |
Colormetric |
| Gene Symbol: |
AQP1 |
| Gene ID: |
358 |
| Uniprot ID: |
AQP1_HUMAN |
| Specificity: |
This kit recognizes Human AQP-1 in samples. No significant cross-reactivity or interference between Human AQP-1 and analogues was observed. |
| Sample Type: |
Serum, plasma and other biological fluids |
| Function | Forms a water-specific channel that provides the plasma membranes of red cells and kidney proximal tubules with high permeability to water, thereby permitting water to move in the direction of an osmotic gradient. Component of the ankyrin-1 complex, a multiprotein complex involved in the stability and shape of the erythrocyte membrane. |
| Protein Name | Aquaporin-1Aqp-1Aquaporin-ChipUrine Water ChannelWater Channel Protein For Red Blood Cells And Kidney Proximal Tubule |
| Database Links | Reactome: R-HSA-1237044Reactome: R-HSA-1247673Reactome: R-HSA-432040Reactome: R-HSA-432047 |
| Cellular Localisation | Cell MembraneMulti-Pass Membrane Protein |
| Alternative ELISA Names | Aquaporin-1 ELISA kitAqp-1 ELISA kitAquaporin-Chip ELISA kitUrine Water Channel ELISA kitWater Channel Protein For Red Blood Cells And Kidney Proximal Tubule ELISA kitAQP1 ELISA kitCHIP28 ELISA kit |
| Specificity | This kit recognizes Human AQP-1 in samples. No significant cross-reactivity or interference between Human AQP-1 and analogues was observed. |
| Reproducibility | Both intra-CV and inter-CV are |
Information sourced from Uniprot.org
| Item | Specifications | Storage |
| Micro ELISA Plate (Dismountable) | 96T: 8 wells ×12 strips strips | -20℃, 6 months |
| Reference Standard | 96T: 2 vials 48T: 1 vial | -20℃, 6 months |
| Concentrated Biotinylated Detection Ab (100×) | 96T: 1 vial, 120 μL 60 μL | -20℃, 6 months |
| Concentrated HRP Conjugate (100×) | 96T: 1 vial, 120 μL 60 μL | -20℃ (Protect from light), 6 months |
| Reference Standard & Sample Diluent | 1 vial, 20 mL | 2-8°C, 6 months |
| Biotinylated Detection Ab Diluent | 1 vial, 14 mL | 2-8°C, 6 months |
| HRP Conjugate Diluent | 1 vial, 14 mL | 2-8°C, 6 months |
| Concentrated Wash Buffer (25×) | 1 vial, 30 mL | 2-8°C, 6 months |
| Substrate Reagent | 1 vial, 10 mL | 2-8℃ (Protect from light) |
| Stop Solution | 1 vial, 10 mL | 2-8°C |
| Plate Sealer | 5 pieces | |
| Manual | 1 copy | |
| Certificate of Analysis | 1 copy | |
| Sample Type | Range (%) | Average Recovery (%) |
| Serum(n=8) | 93-108 | 99 |
| EDTA plasma (n=8) | 87-100 | 93 |
| Cell culture media (n=8) | 92-103 | 98 |
| | Intra-assay Precision | Intra-assay Precision | Intra-assay Precision | Inter-assay Precision | Inter-assay Precision | Inter-assay Precision |
| Sample | 1.00 | 2.00 | 3.00 | 1.00 | 2.00 | 3.00 |
| n | 20.00 | 20.00 | 20.00 | 20.00 | 20.00 | 20.00 |
| Mean (pg/mL) | 399.90 | 1101.92 | 3738.97 | 437.93 | 1190.85 | 3925.00 |
| Standard deviation | 20.03 | 59.28 | 118.15 | 26.54 | 49.18 | 174.27 |
| CV (%) | 5.01 | 5.38 | 3.16 | 6.06 | 4.13 | 4.44 |
12 months for antibodies. 6 months for ELISA Kits. Please see website T&Cs for further guidance
