| Applications: |
CLIA |
| Reactivity: |
Human |
| Note: |
FOR SCIENTIFIC EDUCATIONAL RESEARCH USE ONLY (RUO). MUST NOT BE USED IN DIAGNOSTIC OR OTHER MEDICAL APPLICATIONS. |
| Sensitivity: |
18.75pg/mL |
| Detection Limit: |
31.25~2000pg/mL |
| Short Description: |
This human AQP1 kit is a highly sensitive in-vitro chemiluminescent immunoassay for the measurement of trace amounts of analytes. |
| Storage Instruction: |
If unopened the kit may be stored at 2-8°C for up to 1 month. If the kit will not be used within 1 month, store the components separately, according to the component table in the manual. |
| Assay Time: |
3.5h |
| Detection: |
Chemiluminescence |
| Gene Symbol: |
AQP1 |
| Gene ID: |
358 |
| Uniprot ID: |
AQP1_HUMAN |
| Specificity: |
This kit recognizes Human AQP-1 in samples. No significant cross-reactivity or interference between Human AQP-1 and analogues was observed. |
| Sample Type: |
Serum, plasma and other biological fluids |
| Tissue Specificity | Detected in erythrocytes (at protein level). Expressed in a number of tissues including erythrocytes, renal tubules, retinal pigment epithelium, heart, lung, skeletal muscle, kidney and pancreas. Weakly expressed in brain, placenta and liver. |
| Function | Forms a water-specific channel that provides the plasma membranes of red cells and kidney proximal tubules with high permeability to water, thereby permitting water to move in the direction of an osmotic gradient. Component of the ankyrin-1 complex, a multiprotein complex involved in the stability and shape of the erythrocyte membrane. |
| Protein Name | Aquaporin-1Aqp-1Aquaporin-ChipUrine Water ChannelWater Channel Protein For Red Blood Cells And Kidney Proximal Tubule |
| Database Links | Reactome: R-HSA-1237044Reactome: R-HSA-1247673Reactome: R-HSA-432040Reactome: R-HSA-432047 |
| Cellular Localisation | Cell MembraneMulti-Pass Membrane Protein |
| Alternative CLIA Names | Aquaporin-1 CLIA kitAqp-1 CLIA kitAquaporin-Chip CLIA kitUrine Water Channel CLIA kitWater Channel Protein For Red Blood Cells And Kidney Proximal Tubule CLIA kitAQP1 CLIA kitCHIP28 CLIA kit |
| Specificity | This kit recognizes Human AQP-1 in samples. No significant cross-reactivity or interference between Human AQP-1 and analogues was observed. |
| Reproducibility | Both intra-CV and inter-CV are |
Information sourced from Uniprot.org
| Item | Specifications | Storage |
| Micro CLIA Plate (Dismountable) | 96T: 8 wells ×12 strips strips | -20℃, 6 months |
| Reference Standard | 96T: 2 vials 48T: 1 vial | -20℃, 6 months |
| Concentrated Biotinylated Detection Ab (100×) | 96T: 1 vial, 120 μL 60 μL | -20℃, 6 months |
| Concentrated HRP Conjugate (100×) | 96T: 1 vial, 120 μL 60 μL | -20℃ (Protect from light), 6 months |
| Reference Standard & Sample Diluent | 1 vial, 20 mL | 2-8°C, 6 months |
| Biotinylated Detection Ab Diluent | 1 vial, 14 mL | 2-8°C, 6 months |
| HRP Conjugate Diluent | 1 vial, 14 mL | 2-8°C, 6 months |
| Concentrated Wash Buffer (25×) | 1 vial, 30 mL | 2-8°C, 6 months |
| Substrate Reagent A | 1 vial, 5 mL | 2-8℃ (Protect from light) |
| Substrate Reagent B | 1 vial, 5 mL | 2-8℃ (Protect from light) |
| Plate Sealer | 5 pieces | |
| Manual | 1 copy | |
| Certificate of Analysis | 1 copy | |
| Sample Type | Range (%) | Average Recovery (%) |
| Serum(n=8) | 87-101 | 94 |
| EDTA plasma(n=8) | 90-101 | 95 |
| Cell culture media(n=8) | 90-105 | 98 |
| | Intra-assay Precision | Intra-assay Precision | Intra-assay Precision | Inter-assay Precision | Inter-assay Precision | Inter-assay Precision |
| Sample | 1.00 | 2.00 | 3.00 | 1.00 | 2.00 | 3.00 |
| n | 20.00 | 20.00 | 20.00 | 20.00 | 20.00 | 20.00 |
| Mean (pg/mL) | 99.35 | 291.60 | 891.14 | 101.49 | 299.18 | 910.82 |
| Standard deviation | 12.49 | 28.11 | 56.59 | 10.82 | 30.16 | 79.24 |
| CV (%) | 12.57 | 9.64 | 6.35 | 10.66 | 10.08 | 8.70 |
12 months for antibodies. 6 months for ELISA Kits. Please see website T&Cs for further guidance
