| Applications: |
ELISA |
| Reactivity: |
Human |
| Note: |
FOR SCIENTIFIC EDUCATIONAL RESEARCH USE ONLY (RUO). MUST NOT BE USED IN DIAGNOSTIC OR OTHER MEDICAL APPLICATIONS. |
| Sensitivity: |
0.19ng/mL |
| Detection Limit: |
0.31~20ng/mL |
| Short Description: |
This AQP4 Sandwich ELISA is an in-vitro enzyme-linked immunosorbent assay for the measurement of samples in human cell culture supernatant, serum and plasma (EDTA, citrate, heparin). |
| Storage Instruction: |
If unopened the kit may be stored at 2-8°C for up to 1 month. If the kit will not be used within 1 month, store the components separately, according to the component table in the manual. |
| Assay Time: |
3.5h |
| Detection: |
Colormetric |
| Gene Symbol: |
AQP4 |
| Gene ID: |
361 |
| Uniprot ID: |
AQP4_HUMAN |
| Specificity: |
This kit recognizes Human AQP-4 in samples. No significant cross-reactivity or interference between Human AQP-4 and analogues was observed. |
| Sample Type: |
Serum, plasma and other biological fluids |
| Post Translational Modifications | Phosphorylation by PKC at Ser-180 reduces conductance by 50%. Phosphorylation by PKG at Ser-111 in response to glutamate increases conductance by 40%. Isoform 2: Palmitoylated on its N-terminal region. Isoform 1: Not palmitoylated. |
| Function | Forms a water-specific channel. Plays an important role in brain water homeostasis and in glymphatic solute transport. Required for a normal rate of water exchange across the blood brain interface. Required for normal levels of cerebrospinal fluid influx into the brain cortex and parenchyma along paravascular spaces that surround penetrating arteries, and for normal drainage of interstitial fluid along paravenous drainage pathways. Thereby, it is required for normal clearance of solutes from the brain interstitial fluid, including soluble beta-amyloid peptides derived from APP. Plays a redundant role in urinary water homeostasis and urinary concentrating ability. |
| Protein Name | Aquaporin-4Aqp-4Mercurial-Insensitive Water ChannelMiwcWch4 |
| Database Links | Reactome: R-HSA-432040Reactome: R-HSA-432047 |
| Cellular Localisation | Cell MembraneMulti-Pass Membrane ProteinBasolateral Cell MembraneEndosome MembraneSarcolemmaCell ProjectionActivation Of The Vasopressin Receptor Avpr1a Triggers Aqp4 Phosphorylation At Ser-180 And Promotes Its Internalization From The Cell MembraneDetected On Brain Astrocyte Processes And Astrocyte Endfeet Close To Capillaries |
| Alternative ELISA Names | Aquaporin-4 ELISA kitAqp-4 ELISA kitMercurial-Insensitive Water Channel ELISA kitMiwc ELISA kitWch4 ELISA kitAQP4 ELISA kit |
| Specificity | This kit recognizes Human AQP-4 in samples. No significant cross-reactivity or interference between Human AQP-4 and analogues was observed. |
| Reproducibility | Both intra-CV and inter-CV are |
Information sourced from Uniprot.org
| Item | Specifications | Storage |
| Micro ELISA Plate (Dismountable) | 96T: 8 wells ×12 strips strips | -20℃, 6 months |
| Reference Standard | 96T: 2 vials 48T: 1 vial | -20℃, 6 months |
| Concentrated Biotinylated Detection Ab (100×) | 96T: 1 vial, 120 μL 60 μL | -20℃, 6 months |
| Concentrated HRP Conjugate (100×) | 96T: 1 vial, 120 μL 60 μL | -20℃ (Protect from light), 6 months |
| Reference Standard & Sample Diluent | 1 vial, 20 mL | 2-8°C, 6 months |
| Biotinylated Detection Ab Diluent | 1 vial, 14 mL | 2-8°C, 6 months |
| HRP Conjugate Diluent | 1 vial, 14 mL | 2-8°C, 6 months |
| Concentrated Wash Buffer (25×) | 1 vial, 30 mL | 2-8°C, 6 months |
| Substrate Reagent | 1 vial, 10 mL | 2-8℃ (Protect from light) |
| Stop Solution | 1 vial, 10 mL | 2-8°C |
| Plate Sealer | 5 pieces | |
| Manual | 1 copy | |
| Certificate of Analysis | 1 copy | |
| Sample Type | Range (%) | Average Recovery (%) |
| Serum(n=8) | 89-103 | 96 |
| EDTA plasma (n=8) | 95-107 | 100 |
| Cell culture media (n=8) | 90-106 | 98 |
| | Intra-assay Precision | Intra-assay Precision | Intra-assay Precision | Inter-assay Precision | Inter-assay Precision | Inter-assay Precision |
| Sample | 1.00 | 2.00 | 3.00 | 1.00 | 2.00 | 3.00 |
| n | 20.00 | 20.00 | 20.00 | 20.00 | 20.00 | 20.00 |
| Mean (ng/mL) | 1.01 | 3.21 | 8.08 | 0.92 | 3.20 | 8.88 |
| Standard deviation | 0.06 | 0.14 | 0.42 | 0.06 | 0.15 | 0.35 |
| CV (%) | 5.94 | 4.36 | 5.20 | 6.52 | 4.69 | 3.94 |
12 months for antibodies. 6 months for ELISA Kits. Please see website T&Cs for further guidance
