| Applications: |
ELISA |
| Reactivity: |
Human |
| Note: |
FOR SCIENTIFIC EDUCATIONAL RESEARCH USE ONLY (RUO). MUST NOT BE USED IN DIAGNOSTIC OR OTHER MEDICAL APPLICATIONS. |
| Sensitivity: |
0.38ng/mL |
| Detection Limit: |
0.63~40ng/mL |
| Short Description: |
This ANXA2 Sandwich ELISA is an in-vitro enzyme-linked immunosorbent assay for the measurement of samples in human cell culture supernatant, serum and plasma (EDTA, citrate, heparin). |
| Storage Instruction: |
If unopened the kit may be stored at 2-8°C for up to 1 month. If the kit will not be used within 1 month, store the components separately, according to the component table in the manual. |
| Assay Time: |
3.5h |
| Detection: |
Colormetric |
| Gene Symbol: |
ANXA2 |
| Gene ID: |
302 |
| Uniprot ID: |
ANXA2_HUMAN |
| Specificity: |
This kit recognizes Human ANXA2 in samples. No significant cross-reactivity or interference between Human ANXA2 and analogues was observed. |
| Sample Type: |
Serum, plasma and other biological fluids |
| Function | Calcium-regulated membrane-binding protein whose affinity for calcium is greatly enhanced by anionic phospholipids. It binds two calcium ions with high affinity. May be involved in heat-stress response. Inhibits PCSK9-enhanced LDLR degradation, probably reduces PCSK9 protein levels via a translational mechanism but also competes with LDLR for binding with PCSK9. (Microbial infection) Binds M.pneumoniae CARDS toxin, probably serves as one receptor for this pathogen. When ANXA2 is down-regulated by siRNA, less toxin binds to human cells and less vacuolization (a symptom of M.pneumoniae infection) is seen. |
| Protein Name | Annexin A2Annexin IiAnnexin-2Calpactin I Heavy ChainCalpactin-1 Heavy ChainChromobindin-8Lipocortin IiPlacental Anticoagulant Protein IvPap-IvProtein IP36 |
| Database Links | Reactome: R-HSA-445355Reactome: R-HSA-6798695Reactome: R-HSA-75205Reactome: R-HSA-8950505 |
| Cellular Localisation | SecretedExtracellular SpaceExtracellular MatrixBasement MembraneMelanosomeIn The Lamina Beneath The Plasma MembraneIdentified By Mass Spectrometry In Melanosome Fractions From Stage I To Stage IvTranslocated From The Cytoplasm To The Cell Surface Through A Golgi-Independent Mechanism |
| Alternative ELISA Names | Annexin A2 ELISA kitAnnexin Ii ELISA kitAnnexin-2 ELISA kitCalpactin I Heavy Chain ELISA kitCalpactin-1 Heavy Chain ELISA kitChromobindin-8 ELISA kitLipocortin Ii ELISA kitPlacental Anticoagulant Protein Iv ELISA kitPap-Iv ELISA kitProtein I ELISA kitP36 ELISA kitANXA2 ELISA kitANX2 ELISA kitANX2L4 ELISA kitCAL1H ELISA kitLPC2D ELISA kit |
| Specificity | This kit recognizes Human ANXA2 in samples. No significant cross-reactivity or interference between Human ANXA2 and analogues was observed. |
| Reproducibility | Both intra-CV and inter-CV are |
Information sourced from Uniprot.org
| Item | Specifications | Storage |
| Micro ELISA Plate (Dismountable) | 96T: 8 wells ×12 strips strips | -20℃, 6 months |
| Reference Standard | 96T: 2 vials 48T: 1 vial | -20℃, 6 months |
| Concentrated Biotinylated Detection Ab (100×) | 96T: 1 vial, 120 μL 60 μL | -20℃, 6 months |
| Concentrated HRP Conjugate (100×) | 96T: 1 vial, 120 μL 60 μL | -20℃ (Protect from light), 6 months |
| Reference Standard & Sample Diluent | 1 vial, 20 mL | 2-8°C, 6 months |
| Biotinylated Detection Ab Diluent | 1 vial, 14 mL | 2-8°C, 6 months |
| HRP Conjugate Diluent | 1 vial, 14 mL | 2-8°C, 6 months |
| Concentrated Wash Buffer (25×) | 1 vial, 30 mL | 2-8°C, 6 months |
| Substrate Reagent | 1 vial, 10 mL | 2-8℃ (Protect from light) |
| Stop Solution | 1 vial, 10 mL | 2-8°C |
| Plate Sealer | 5 pieces | |
| Manual | 1 copy | |
| Certificate of Analysis | 1 copy | |
| Sample Type | Range (%) | Average Recovery (%) |
| Serum(n=8) | 88-102 | 94 |
| EDTA plasma (n=8) | 93-108 | 99 |
| Cell culture media (n=8) | 92-107 | 98 |
| | Intra-assay Precision | Intra-assay Precision | Intra-assay Precision | Inter-assay Precision | Inter-assay Precision | Inter-assay Precision |
| Sample | 1.00 | 2.00 | 3.00 | 1.00 | 2.00 | 3.00 |
| n | 20.00 | 20.00 | 20.00 | 20.00 | 20.00 | 20.00 |
| Mean (ng/mL) | 2.02 | 3.69 | 18.13 | 1.96 | 3.63 | 18.51 |
| Standard deviation | 0.14 | 0.20 | 0.94 | 0.14 | 0.16 | 0.78 |
| CV (%) | 6.93 | 5.42 | 5.18 | 7.14 | 4.41 | 4.21 |
12 months for antibodies. 6 months for ELISA Kits. Please see website T&Cs for further guidance
