Applications: | ELISA |
Reactivity: | Human |
Note: | FOR SCIENTIFIC EDUCATIONAL RESEARCH USE ONLY (RUO). MUST NOT BE USED IN DIAGNOSTIC OR OTHER MEDICAL APPLICATIONS. |
Sensitivity: | 0.38ng/mL |
Detection Limit: | 0.63~40ng/mL |
Short Description: | This ANXA2 Sandwich ELISA is an in-vitro enzyme-linked immunosorbent assay for the measurement of samples in human cell culture supernatant, serum and plasma (EDTA, citrate, heparin). |
Storage Instruction: | If unopened the kit may be stored at 2-8°C for up to 1 month. If the kit will not be used within 1 month, store the components separately, according to the component table in the manual. |
Assay Time: | 3.5h |
Detection: | Colormetric |
Gene Symbol: | ANXA2 |
Gene ID: | 302 |
Uniprot ID: | ANXA2_HUMAN |
Specificity: | This kit recognizes Human ANXA2 in samples. No significant cross-reactivity or interference between Human ANXA2 and analogues was observed. |
Sample Type: | Serum, plasma and other biological fluids |
Function | Calcium-regulated membrane-binding protein whose affinity for calcium is greatly enhanced by anionic phospholipids. It binds two calcium ions with high affinity. May be involved in heat-stress response. Inhibits PCSK9-enhanced LDLR degradation, probably reduces PCSK9 protein levels via a translational mechanism but also competes with LDLR for binding with PCSK9. (Microbial infection) Binds M.pneumoniae CARDS toxin, probably serves as one receptor for this pathogen. When ANXA2 is down-regulated by siRNA, less toxin binds to human cells and less vacuolization (a symptom of M.pneumoniae infection) is seen. |
Protein Name | Annexin A2 Annexin Ii Annexin-2 Calpactin I Heavy Chain Calpactin-1 Heavy Chain Chromobindin-8 Lipocortin Ii Placental Anticoagulant Protein Iv Pap-Iv Protein I P36 |
Database Links | Reactome: R-HSA-445355 Reactome: R-HSA-6798695 Reactome: R-HSA-75205 Reactome: R-HSA-8950505 |
Cellular Localisation | Secreted Extracellular Space Extracellular Matrix Basement Membrane Melanosome In The Lamina Beneath The Plasma Membrane Identified By Mass Spectrometry In Melanosome Fractions From Stage I To Stage Iv Translocated From The Cytoplasm To The Cell Surface Through A Golgi-Independent Mechanism |
Alternative ELISA Names | Annexin A2 ELISA kit Annexin Ii ELISA kit Annexin-2 ELISA kit Calpactin I Heavy Chain ELISA kit Calpactin-1 Heavy Chain ELISA kit Chromobindin-8 ELISA kit Lipocortin Ii ELISA kit Placental Anticoagulant Protein Iv ELISA kit Pap-Iv ELISA kit Protein I ELISA kit P36 ELISA kit ANXA2 ELISA kit ANX2 ELISA kit ANX2L4 ELISA kit CAL1H ELISA kit LPC2D ELISA kit |
Specificity | This kit recognizes Human ANXA2 in samples. No significant cross-reactivity or interference between Human ANXA2 and analogues was observed. |
Reproducibility | Both intra-CV and inter-CV are |
Information sourced from Uniprot.org
Item | Specifications | Storage |
---|---|---|
Micro ELISA Plate (Dismountable) | 96T: 8 wells ×12 strips strips | -20℃, 6 months |
Reference Standard | 96T: 2 vials 48T: 1 vial | -20℃, 6 months |
Concentrated Biotinylated Detection Ab (100×) | 96T: 1 vial, 120 μL 60 μL | -20℃, 6 months |
Concentrated HRP Conjugate (100×) | 96T: 1 vial, 120 μL 60 μL | -20℃ (Protect from light), 6 months |
Reference Standard & Sample Diluent | 1 vial, 20 mL | 2-8°C, 6 months |
Biotinylated Detection Ab Diluent | 1 vial, 14 mL | 2-8°C, 6 months |
HRP Conjugate Diluent | 1 vial, 14 mL | 2-8°C, 6 months |
Concentrated Wash Buffer (25×) | 1 vial, 30 mL | 2-8°C, 6 months |
Substrate Reagent | 1 vial, 10 mL | 2-8℃ (Protect from light) |
Stop Solution | 1 vial, 10 mL | 2-8°C |
Plate Sealer | 5 pieces | |
Manual | 1 copy | |
Certificate of Analysis | 1 copy |
Sample Type | Range (%) | Average Recovery (%) |
---|---|---|
Serum(n=8) | 88-102 | 94 |
EDTA plasma (n=8) | 93-108 | 99 |
Cell culture media (n=8) | 92-107 | 98 |
Intra-assay Precision | Intra-assay Precision | Intra-assay Precision | Inter-assay Precision | Inter-assay Precision | Inter-assay Precision | |
---|---|---|---|---|---|---|
Sample | 1.00 | 2.00 | 3.00 | 1.00 | 2.00 | 3.00 |
n | 20.00 | 20.00 | 20.00 | 20.00 | 20.00 | 20.00 |
Mean (ng/mL) | 2.02 | 3.69 | 18.13 | 1.96 | 3.63 | 18.51 |
Standard deviation | 0.14 | 0.20 | 0.94 | 0.14 | 0.16 | 0.78 |
CV (%) | 6.93 | 5.42 | 5.18 | 7.14 | 4.41 | 4.21 |