| Host: |
Rabbit |
| Applications: |
WB |
| Reactivity: |
Human |
| Note: |
STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS. |
| Short Description: |
Rabbit polyclonal antibody anti-VRK2 (269-508) is suitable for use in Western Blot research applications. |
| Clonality: |
Polyclonal |
| Conjugation: |
Unconjugated |
| Isotype: |
IgG |
| Formulation: |
PBS with 0.01% Thimerosal, 50% Glycerol, pH7.3. |
| Purification: |
Affinity purification |
| Dilution Range: |
WB 1:500-1:2000 |
| Storage Instruction: |
Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles. |
| Gene Symbol: |
VRK2 |
| Gene ID: |
7444 |
| Uniprot ID: |
VRK2_HUMAN |
| Immunogen Region: |
269-508 |
| Immunogen: |
Recombinant fusion protein containing a sequence corresponding to amino acids 269-508 of human VRK2 (NP_006287.2). |
| Immunogen Sequence: |
DELPQSVLKWAPSGSSCCEI AQFLVCAHSLAYDEKPNYQA LKKILNPHGIPLGPLDFSTK GQSINVHTPNSQKVDSQKAA TKQVNKAHNRLIEKKVHSER SAESCATWKVQKEEKLIGLM NNEAAQESTRRRQKYQESQE PLNEVNSFPQKISYTQFPNS FYEPHQDFTSPDIFKKSRSP SWYKYTSTVSTGITDLESST GLWPTISQFTLSEETNADVY YYRIIIPVLLMLVFLALFF |
| Tissue Specificity | Isoform 1 and isoform 2 are expressed in various tumor cell lines. Expression of isoform 1 inversely correlates with ERBB2 in breast carcinomas (at protein level). Widely expressed. Highly expressed in fetal liver, skeletal muscle, pancreas, heart, peripheral blood leukocytes and testis. |
| Post Translational Modifications | Isoform 1: Autophosphorylated. Isoform 2: Autophosphorylated. |
| Function | Serine/threonine kinase that regulates several signal transduction pathways. Isoform 1 modulates the stress response to hypoxia and cytokines, such as interleukin-1 beta (IL1B) and this is dependent on its interaction with MAPK8IP1, which assembles mitogen-activated protein kinase (MAPK) complexes. Inhibition of signal transmission mediated by the assembly of MAPK8IP1-MAPK complexes reduces JNK phosphorylation and JUN-dependent transcription. Phosphorylates 'Thr-18' of p53/TP53, histone H3, and may also phosphorylate MAPK8IP1. Phosphorylates BANF1 and disrupts its ability to bind DNA and reduces its binding to LEM domain-containing proteins. Down-regulates the transactivation of transcription induced by ERBB2, HRAS, BRAF, and MEK1. Blocks the phosphorylation of ERK in response to ERBB2 and HRAS. Can also phosphorylate the following substrates that are commonly used to establish in vitro kinase activity: casein, MBP and histone H2B, but it is not sure that this is physiologically relevant. Isoform 2: Phosphorylates 'Thr-18' of p53/TP53, as well as histone H3. Reduces p53/TP53 ubiquitination by MDM2, promotes p53/TP53 acetylation by EP300 and thereby increases p53/TP53 stability and activity. |
| Protein Name | Serine/Threonine-Protein Kinase Vrk2Vaccinia-Related Kinase 2 |
| Database Links | Reactome: R-HSA-2980766 Q86Y07-2Reactome: R-HSA-2995383 Q86Y07-2Reactome: R-HSA-9013149Reactome: R-HSA-9013404Reactome: R-HSA-9013405Reactome: R-HSA-9013408Reactome: R-HSA-9013423 |
| Cellular Localisation | Isoform 1: CytoplasmEndoplasmic Reticulum MembraneSingle-Pass Type Iv Membrane ProteinMitochondrion MembraneNucleus EnvelopeIsoform 2: CytoplasmNucleus |
| Alternative Antibody Names | Anti-Serine/Threonine-Protein Kinase Vrk2 antibodyAnti-Vaccinia-Related Kinase 2 antibodyAnti-VRK2 antibody |
Information sourced from Uniprot.org
12 months for antibodies. 6 months for ELISA Kits. Please see website T&Cs for further guidance
