• Western blot analysis of extracts of various cell lines, using UBE2U antibody (STJ110674) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% non-fat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 30s.
  • Immunohistochemistry analysis of paraffin-embedded mouse brain using UBE2U antibody (STJ110674) at dilution of 1:100 (40x lens). Perform microwave antigen retrieval with 10 mM PBS buffer pH 7. 2 before commencing with immunohistochemistry staining protocol.
  • Immunohistochemistry of paraffin-embedded human liver using UBE2U antibody (STJ110674) at dilution of 1:100 (40x lens).
  • Immunohistochemistry analysis of paraffin-embedded human liver using UBE2U antibody (STJ110674) at dilution of 1:100 (40x lens). Perform microwave antigen retrieval with 10 mM PBS buffer pH 7. 2 before commencing with immunohistochemistry staining protocol.
  • Immunohistochemistry of paraffin-embedded mouse brain using UBE2U antibody (STJ110674) at dilution of 1:100 (40x lens).
  • Western blot analysis of extracts of various cell lines, using UBE2U antibody (STJ110674) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 30s.

Anti-UBE2U antibody (1-226) (STJ110674)

SKU:
STJ110674

£112.50 - £524.50
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Host: Rabbit
Applications: WB/IHC-P/ELISA
Reactivity: Human/Mouse
Note: STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS.
Clonality: Polyclonal
Conjugation: Unconjugated
Isotype: IgG
Formulation: PBS with 0.02% Sodium Azide, 50% Glycerol, pH 7.3.
Purification: Affinity purification
Concentration: Lot specific
Dilution Range: WB:1:500-1:2000
IHC-P:1:50-1:100
ELISA:Recommended starting concentration is 1 Mu g/mL. Please optimize the concentration based on your specific assay requirements.
Storage Instruction: Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles.
Gene Symbol: UBE2U
Gene ID: 148581
Uniprot ID: UBE2U_HUMAN
Immunogen Region: 1-226
Specificity: Recombinant fusion protein containing a sequence corresponding to amino acids 1-226 of human UBE2U (NP_689702.1).
Immunogen Sequence: MHGRAYLLLHRDFCDLKENN YKGITAKPVSEDMMEWEVEI EGLQNSVWQGLVFQLTIHFT SEYNYAPPVVKFITIPFHPN VDPHTGQPCIDFLDNPEKWN TNYTLSSILLALQVMLSNPV LENPVNLEAARILVKDESLY RTILRLFNRPLQMKDDSQEL PKDPRKCIRPIKTTSFSDYY QTWSRIATSKATEYYRTPLL KVPNFIGQYYKWKKMDLQHQ KEWNLK
Post Translational Modifications Autoubiquitinated in vitro in the presence of UBR5.
Function Catalyzes the covalent attachment of ubiquitin to other proteins.
Protein Name Ubiquitin-Conjugating Enzyme E2 U
E2 Ubiquitin-Conjugating Enzyme U
Ubiquitin Carrier Protein U
Ubiquitin-Protein Ligase U
Database Links Reactome: R-HSA-983168
Alternative Antibody Names Anti-Ubiquitin-Conjugating Enzyme E2 U antibody
Anti-E2 Ubiquitin-Conjugating Enzyme U antibody
Anti-Ubiquitin Carrier Protein U antibody
Anti-Ubiquitin-Protein Ligase U antibody
Anti-UBE2U antibody

Information sourced from Uniprot.org

12 months for antibodies. 6 months for ELISA Kits. Please see website T&Cs for further guidance