• Western blot analysis of extracts of various cell lines, using TTL antibody (STJ112067) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 10s.

Anti-TTL antibody (1-270) (STJ112067)

SKU:
STJ112067

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Host: Rabbit
Applications: WB
Reactivity: Human/Mouse/Rat
Note: STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS.
Short Description: Rabbit polyclonal antibody anti-TTL (1-270) is suitable for use in Western Blot research applications.
Clonality: Polyclonal
Conjugation: Unconjugated
Isotype: IgG
Formulation: PBS with 0.02% Sodium Azide, 50% Glycerol, pH7.3.
Purification: Affinity purification
Dilution Range: WB 1:1000-1:4000
Storage Instruction: Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles.
Gene Symbol: TTL
Gene ID: 150465
Uniprot ID: TTL_HUMAN
Immunogen Region: 1-270
Immunogen: Recombinant fusion protein containing a sequence corresponding to amino acids 1-270 of human TTL (NP_714923.1).
Immunogen Sequence: MYTFVVRDENSSVYAEVSRL LLATGHWKRLRRDNPRFNLM LGERNRLPFGRLGHEPGLVQ LVNYYRGADKLCRKASLVKL IKTSPELAESCTWFPESYVI YPTNLKTPVAPAQNGIQPPI SNSRTDEREFFLASYNRKKE DGEGNVWIAKSSAGAKGEGI LISSEASELLDFIDNQGQVH VIQKYLEHPLLLEPGHRKFD IRSWVLVDHQYNIYLYREGV LRTASEPYHVDNFQDKTCH
Function Catalyzes the post-translational addition of a tyrosine to the C-terminal end of detyrosinated alpha-tubulin.
Protein Name Tubulin--Tyrosine Ligase
Ttl
Database Links Reactome: R-HSA-8955332
Alternative Antibody Names Anti-Tubulin--Tyrosine Ligase antibody
Anti-Ttl antibody
Anti-TTL antibody

Information sourced from Uniprot.org

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