• Western blot analysis of various lysates, using TPI1 Rabbit polyclonal antibody (STJ11104642) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 10s.

Anti-TPI1 antibody (1-249) (STJ11104642)

SKU:
STJ11104642

£112.50 - £522.50
Current Stock:
Host: Rabbit
Applications: WB
Reactivity: Human/Mouse/Rat
Note: STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS.
Short Description: Rabbit polyclonal antibody anti-Triosephosphate Isomerase (1-249) is suitable for use in Western Blot research applications.
Clonality: Polyclonal
Conjugation: Unconjugated
Isotype: IgG
Formulation: PBS with 0.05% Proclin300, 50% Glycerol, pH7.3.
Purification: Affinity purification
Dilution Range: WB 1:500-1:1000
Storage Instruction: Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles.
Gene Symbol: TPI1
Gene ID: 7167
Uniprot ID: TPIS_HUMAN
Immunogen Region: 1-249
Immunogen: Recombinant fusion protein containing a sequence corresponding to amino acids 1-249 of human TPI1 (NP_000356.1).
Immunogen Sequence: MAPSRKFFVGGNWKMNGRKQ SLGELIGTLNAAKVPADTEV VCAPPTAYIDFARQKLDPKI AVAAQNCYKVTNGAFTGEIS PGMIKDCGATWVVLGHSERR HVFGESDELIGQKVAHALAE GLGVIACIGEKLDEREAGIT EKVVFEQTKVIADNVKDWSK VVLAYEPVWAIGTGKTATPQ QAQEVHEKLRGWLKSNVSDA VAQSTRIIYGGSVTGATCKE LASQPDVDGFLVGGASLKP
Function Triosephosphate isomerase is an extremely efficient metabolic enzyme that catalyzes the interconversion between dihydroxyacetone phosphate (DHAP) and D-glyceraldehyde-3-phosphate (G3P) in glycolysis and gluconeogenesis. It is also responsible for the non-negligible production of methylglyoxal a reactive cytotoxic side-product that modifies and can alter proteins, DNA and lipids.
Protein Name Triosephosphate Isomerase
Tim
Methylglyoxal Synthase
Triose-Phosphate Isomerase
Database Links Reactome: R-HSA-70171
Reactome: R-HSA-70263
Cellular Localisation Cytoplasm
Alternative Antibody Names Anti-Triosephosphate Isomerase antibody
Anti-Tim antibody
Anti-Methylglyoxal Synthase antibody
Anti-Triose-Phosphate Isomerase antibody
Anti-TPI1 antibody
Anti-TPI antibody

Information sourced from Uniprot.org

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