• Western blot analysis of extracts of various cell lines, using TIMM50 antibody (STJ112032) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% non-fat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 1s.
  • Western blot analysis of extracts of various cell lines, using TIMM50 antibody (STJ112032) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 1s.

Anti-TIMM50 antibody (190-456) (STJ112032)

SKU:
STJ112032

£169.50 - £449.50
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Host: Rabbit
Applications: WB
Reactivity: Human/Mouse
Note: STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS.
Short Description: Rabbit polyclonal antibody anti-TIMM50 (190-456) is suitable for use in Western Blot research applications.
Clonality: Polyclonal
Conjugation: Unconjugated
Isotype: IgG
Formulation: PBS with 0.02% Sodium Azide, 50% Glycerol, pH7.3.
Purification: Affinity purification
Dilution Range: WB 1:1000-1:4000
Storage Instruction: Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles.
Gene Symbol: TIMM50
Gene ID: 92609
Uniprot ID: TIM50_HUMAN
Immunogen Region: 190-456
Immunogen: Recombinant fusion protein containing a sequence corresponding to amino acids 190-456 of human TIMM50 (NP_001001563.1).
Immunogen Sequence: GNNPVDENGAKIPDEFDNDP ILVQQLRRTYKYFKDYRQMI IEPTSPCLLPDPLQEPYYQP PYTLVLELTGVLLHPEWSLA TGWRFKKRPGIETLFQQLAP LYEIVIFTSETGMTAFPLID SVDPHGFISYRLFRDATRYM DGHHVKDISCLNRDPARVVV VDCKKEAFRLQPYNGVALRP WDGNSDDRVLLDLSAFLKTI ALNGVEDVRTVLEHYALEDD PLAAFKQRQSRLEQEEQQR
Tissue Specificity Widely expressed. Expressed at higher level in brain, kidney and liver (at protein level).
Function Essential component of the TIM23 complex, a complex that mediates the translocation of transit peptide-containing proteins across the mitochondrial inner membrane. Has some phosphatase activity in vitro.however such activity may not be relevant in vivo. Isoform 2: May participate in the release of snRNPs and SMN from the Cajal body.
Protein Name Mitochondrial Import Inner Membrane Translocase Subunit Tim50
Database Links Reactome: R-HSA-1268020
Cellular Localisation Mitochondrion Inner Membrane
Single-Pass Membrane Protein
Isoform 2: Nucleus Speckle
Nuclear And Enriched In Speckles With Snrnps
Alternative Antibody Names Anti-Mitochondrial Import Inner Membrane Translocase Subunit Tim50 antibody
Anti-TIMM50 antibody
Anti-TIM50 antibody
Anti-PRO1512 antibody

Information sourced from Uniprot.org

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