• Western blot analysis of lysates from Mouse kidney, using STK35 Rabbit polyclonal antibody (STJ119852) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 90s.

Anti-STK35 antibody (190-380) (STJ119852)

SKU:
STJ119852

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Host: Rabbit
Applications: WB
Reactivity: Mouse
Note: STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS.
Short Description: Rabbit polyclonal antibody anti-STK35 (190-380) is suitable for use in Western Blot research applications.
Clonality: Polyclonal
Conjugation: Unconjugated
Isotype: IgG
Formulation: PBS with 0.01% Thimerosal, 50% Glycerol, pH7.3.
Purification: Affinity purification
Dilution Range: WB 1:500-1:2000
Storage Instruction: Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles.
Gene Symbol: STK35
Gene ID: 140901
Uniprot ID: STK35_HUMAN
Immunogen Region: 190-380
Immunogen: Recombinant fusion protein containing a sequence corresponding to amino acids 190-380 of human STK35 (NP_543026.2).
Immunogen Sequence: RPEGGGGSARPRYSLLAEIG RGSYGVVYEAVAGRSGARVA VKKIRCDAPENVELALAEFW ALTSLKRRHQNVVQFEECVL QRNGLAQRMSHGNKSSQLYL RLVETSLKGERILGYAEEPC YLWFVMEFCEGGDLNQYVLS RRPDPATNKSFMLQLTSAIA FLHKNHIVHRDLKPDNILIT ERSGTPILKVA
Tissue Specificity Expressed in testis.
Post Translational Modifications Autophosphorylated.
Protein Name Serine/Threonine-Protein Kinase 35
Clp-36-Interacting Kinase 1
Clik-1
Pdlim1-Interacting Kinase 1
Serine/Threonine-Protein Kinase 35 L1
Cellular Localisation Nucleus
Nucleolus
Cytoplasm
When Associated With Pdlim1
It Is Mostly Found In Cytoplasm
Localized To Actin Stress Fibers
However
Pubmed:19756140 Detected Stk35 Only In The Nucleus
And The Presence Of Pdlim1 Had No Influence On Its Location
Alternative Antibody Names Anti-Serine/Threonine-Protein Kinase 35 antibody
Anti-Clp-36-Interacting Kinase 1 antibody
Anti-Clik-1 antibody
Anti-Pdlim1-Interacting Kinase 1 antibody
Anti-Serine/Threonine-Protein Kinase 35 L1 antibody
Anti-STK35 antibody
Anti-CLIK1 antibody
Anti-PDIK1 antibody
Anti-STK35L1 antibody

Information sourced from Uniprot.org

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