• Western blot analysis of extracts of mouse liver, using SERPINF2 antibody (STJ111986) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% non-fat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 30s.
  • Immunohistochemistry analysis of paraffin-embedded rat pancreas using SERPINF2 antibody (STJ111986) at dilution of 1:100 (40x lens). Perform microwave antigen retrieval with 10 mM PBS buffer pH 7. 2 before commencing with immunohistochemistry staining protocol.
  • Immunohistochemistry of paraffin-embedded rat pancreas using SERPINF2 antibody (STJ111986) at dilution of 1:100 (40x lens).
  • Western blot analysis of extracts of Mouse liver, using SERPINF2 antibody (STJ111986) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 30s.

Anti-SERPINF2 antibody (302-491) (STJ111986)

SKU:
STJ111986

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Host: Rabbit
Applications: WB/IHC
Reactivity: Mouse/Rat
Note: STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS.
Short Description: Rabbit polyclonal antibody anti-SERPINF2 (302-491) is suitable for use in Western Blot and Immunohistochemistry research applications.
Clonality: Polyclonal
Conjugation: Unconjugated
Isotype: IgG
Formulation: PBS with 0.02% Sodium Azide, 50% Glycerol, pH7.3.
Purification: Affinity purification
Dilution Range: WB 1:500-1:1000
IHC-P 1:50-1:100
Storage Instruction: Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles.
Gene Symbol: SERPINF2
Gene ID: 5345
Uniprot ID: A2AP_HUMAN
Immunogen Region: 302-491
Immunogen: Recombinant fusion protein containing a sequence corresponding to amino acids 302-491 of human SERPINF2 (NP_001159392.1).
Immunogen Sequence: VPTHFEWNVSQVLANLSWDT LHPPLVWERPTKVRLPKLYL KHQMDLVATLSQLGLQELFQ APDLRGISEQSLVVSGVQHQ STLELSEVGVEAAAATSIAM SRMSLSSFSVNRPFLFFIFE DTTGLPLFVGSVRNPNPSAP RELKEQQDSPGNKDFLQSLK GFPRGDKLFGPDLKLVPPME EDYPQFGSPK
Tissue Specificity Expressed by the liver and secreted in plasma.
Post Translational Modifications Proteolytically cleaved at Pro-39 by both the prolyl endopeptidase FAP form and antiplasmin-cleaving enzyme FAP soluble form to generate mature alpha-2-antiplasmin.
Function Serine protease inhibitor. The major targets of this inhibitor are plasmin and trypsin, but it also inactivates matriptase-3/TMPRSS7 and chymotrypsin.
Protein Name Alpha-2-Antiplasmin
Alpha-2-Ap
Alpha-2-Plasmin Inhibitor
Alpha-2-Pi
Serpin F2
Database Links Reactome: R-HSA-114608
Reactome: R-HSA-75205
Cellular Localisation Secreted
Alternative Antibody Names Anti-Alpha-2-Antiplasmin antibody
Anti-Alpha-2-Ap antibody
Anti-Alpha-2-Plasmin Inhibitor antibody
Anti-Alpha-2-Pi antibody
Anti-Serpin F2 antibody
Anti-SERPINF2 antibody
Anti-AAP antibody
Anti-PLI antibody

Information sourced from Uniprot.org

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