• Western blot analysis of extracts of SGC-7901 cells, using REC8 antibody (STJ111370) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 30s.

Anti-REC8 antibody (1-270) (STJ111370)

SKU:
STJ111370

£169.50 - £449.50
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Host: Rabbit
Applications: WB
Reactivity: Human
Note: STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS.
Short Description: Rabbit polyclonal antibody anti-REC8 (1-270) is suitable for use in Western Blot research applications.
Clonality: Polyclonal
Conjugation: Unconjugated
Isotype: IgG
Formulation: PBS with 0.02% Sodium Azide, 50% Glycerol, pH7.3.
Purification: Affinity purification
Dilution Range: WB 1:500-1:2000
Storage Instruction: Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles.
Gene Symbol: REC8
Gene ID: 9985
Uniprot ID: REC8_HUMAN
Immunogen Region: 1-270
Immunogen: Recombinant fusion protein containing a sequence corresponding to amino acids 1-270 of human REC8 (NP_005123.2).
Immunogen Sequence: MFYYPNVLQRHTGCFATIWL AATRGSRLVKREYLRVNVVK TCEEILNYVLVRVQPPQPGL PRPRFSLYLSAQLQIGVIRV YSQQCQYLVEDIQHILERLH RAQLQIRIDMETELPSLLLP NHLAMMETLEDAPDPFFGMM SVDPRLPSPFDIPQIRHLLE AAIPERVEEIPPEVPTEPRE PERIPVTVLPPEAITILEAE PIRMLEIEGERELPEVSRRE LDLLIAEEEEAILLEIPRL
Tissue Specificity Expressed in testis and thymus. Expressed in the B-cell lines WI-L2-NS and Namalwa (at protein level).
Post Translational Modifications Phosphorylated.
Function Required during meiosis for separation of sister chromatids and homologous chromosomes. Proteolytic cleavage of REC8 on chromosome arms by separin during anaphase I allows for homologous chromosome separation in meiosis I and cleavage of REC8 on centromeres during anaphase II allows for sister chromatid separation in meiosis II.
Protein Name Meiotic Recombination Protein Rec8 Homolog
Cohesin Rec8p
Database Links Reactome: R-HSA-1221632
Cellular Localisation Nucleus
Chromosome
Centromere
In Meiotic Chromosomes
Localized Along Axial Elements In Prophase From The Leptotene To Diplotene Stages
At Later Prophase Stages
Diakinesis And Metaphase I
Localized Along Interstitial Axes Of Chromosomes Including Both Centromere And Arm Regions
No Longer Detected In Arm Regions In Anaphase I But Persists On Centromere Regions Until Metaphase Ii
Localized To Centromeres And Spindle Poles In Endopolyploid Tumor Cells
Alternative Antibody Names Anti-Meiotic Recombination Protein Rec8 Homolog antibody
Anti-Cohesin Rec8p antibody
Anti-REC8 antibody
Anti-REC8L1 antibody

Information sourced from Uniprot.org

12 months for antibodies. 6 months for ELISA Kits. Please see website T&Cs for further guidance