• Immunoprecipitation analysis of 300 Mu g extracts of MCF7 cells using 3 Mu g RBBP5 antibody (STJ29045). Western blot was performed from the immunoprecipitate using RBBP5 antibody (STJ29045) at a dilution of 1:1000.
  • Immunofluorescence analysis of A-549 cells using RBBP5 Rabbit polyclonal antibody (STJ29045). Secondary antibody: Cy3 Goat Anti-Rabbit IgG (H+L) at 1:500 dilution. Blue: DAPI for nuclear staining.
  • Immunohistochemistry analysis of RBBP5 in paraffin-embedded mouse liver using RBBP5 Rabbit polyclonal antibody (STJ29045) at dilution of 1:100 (40x lens). Perform microwave antigen retrieval with 10 mM PBS buffer pH 7. 2 before commencing with immunohistochemistry staining protocol.
  • Immunohistochemistry analysis of RBBP5 in paraffin-embedded human colon carcinoma using RBBP5 Rabbit polyclonal antibody (STJ29045) at dilution of 1:100 (40x lens). Perform microwave antigen retrieval with 10 mM PBS buffer pH 7. 2 before commencing with immunohistochemistry staining protocol.
  • Immunohistochemistry analysis of RBBP5 in paraffin-embedded rat Intestine using RBBP5 Rabbit polyclonal antibody (STJ29045) at dilution of 1:100 (40x lens). Perform microwave antigen retrieval with 10 mM PBS buffer pH 7. 2 before commencing with immunohistochemistry staining protocol.
  • Western blot analysis of various lysates using RBBP5 Rabbit polyclonal antibody (STJ29045) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 10s.

Anti-RBBP5 antibody (319-538) (STJ29045)

SKU:
STJ29045

£169.50 - £449.50
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Host: Rabbit
Applications: WB/IHC/IF/IP
Reactivity: Human/Mouse/Rat
Note: STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS.
Short Description: Rabbit polyclonal antibody anti-RBBP5 (319-538) is suitable for use in Western Blot, Immunohistochemistry, Immunofluorescence and Immunoprecipitation research applications.
Clonality: Polyclonal
Conjugation: Unconjugated
Isotype: IgG
Formulation: PBS with 0.02% Sodium Azide, 50% Glycerol, pH7.3.
Purification: Affinity purification
Dilution Range: WB 1:500-1:2000
IHC-P 1:50-1:200
IF/ICC 1:50-1:100
IP 1:500-1:1000
Storage Instruction: Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles.
Gene Symbol: RBBP5
Gene ID: 5929
Uniprot ID: RBBP5_HUMAN
Immunogen Region: 319-538
Immunogen: Recombinant fusion protein containing a sequence corresponding to amino acids 319-538 of human RBBP5 (NP_005048.2).
Immunogen Sequence: SIWAQNQVENWSAFAPDFKE LDENVEYEERESEFDIEDED KSEPEQTGADAAEDEEVDVT SVDPIAAFCSSDEELEDSKA LLYLPIAPEVEDPEENPYGP PPDAVQTSLMDEGASSEKKR QSSADGSQPPKKKPKTTNIE LQGVPNDEVHPLLGVKGDGK SKKKQAGRPKGSKGKEKDSP FKPKLYKGDRGLPLEGSAKG KVQAELSQPLTAGGAISELL
Tissue Specificity Ubiquitously expressed.
Function In embryonic stem (ES) cells, plays a crucial role in the differentiation potential, particularly along the neural lineage, regulating gene induction and H3 'Lys-4' methylation at key developmental loci, including that mediated by retinoic acid. Does not affect ES cell self-renewal. Component or associated component of some histone methyltransferase complexes which regulates transcription through recruitment of those complexes to gene promoters. As part of the MLL1/MLL complex, involved in mono-, di- and trimethylation at 'Lys-4' of histone H3. Histone H3 'Lys-4' methylation represents a specific tag for epigenetic transcriptional activation. In association with ASH2L and WDR5, stimulates the histone methyltransferase activities of KMT2A, KMT2B, KMT2C, KMT2D, SETD1A and SETD1B.
Protein Name Retinoblastoma-Binding Protein 5
Rbbp-5
Retinoblastoma-Binding Protein Rbq-3
Database Links Reactome: R-HSA-201722
Reactome: R-HSA-3214841
Reactome: R-HSA-3769402
Reactome: R-HSA-5617472
Reactome: R-HSA-8936459
Reactome: R-HSA-8951664
Reactome: R-HSA-9772755
Cellular Localisation Nucleus
Alternative Antibody Names Anti-Retinoblastoma-Binding Protein 5 antibody
Anti-Rbbp-5 antibody
Anti-Retinoblastoma-Binding Protein Rbq-3 antibody
Anti-RBBP5 antibody
Anti-RBQ3 antibody

Information sourced from Uniprot.org

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