| Host: |
Rabbit |
| Applications: |
WB |
| Reactivity: |
Human/Mouse |
| Note: |
STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS. |
| Short Description: |
Rabbit polyclonal antibody anti-RAP2A (1-183) is suitable for use in Western Blot research applications. |
| Clonality: |
Polyclonal |
| Conjugation: |
Unconjugated |
| Isotype: |
IgG |
| Formulation: |
PBS with 0.02% Sodium Azide, 50% Glycerol, pH7.3. |
| Purification: |
Affinity purification |
| Dilution Range: |
WB 1:500-1:1000 |
| Storage Instruction: |
Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles. |
| Gene Symbol: |
RAP2A |
| Gene ID: |
5911 |
| Uniprot ID: |
RAP2A_HUMAN |
| Immunogen Region: |
1-183 |
| Immunogen: |
Recombinant fusion protein containing a sequence corresponding to amino acids 1-183 of human RAP2A (NP_066361.1). |
| Immunogen Sequence: |
MREYKVVVLGSGGVGKSALT VQFVTGTFIEKYDPTIEDFY RKEIEVDSSPSVLEILDTAG TEQFASMRDLYIKNGQGFIL VYSLVNQQSFQDIKPMRDQI IRVKRYEKVPVILVGNKVDL ESEREVSSSEGRALAEEWGC PFMETSAKSKTMVDELFAEI VRQMNYAAQPDKDDPCCSAC NIQ |
| Post Translational Modifications | Ubiquitinated.undergoes 'Lys-63' monoubiquitination and diubiquitination by NEDD4. Multiple lysine residues are probably modified. Ubiquitination requires TNIK, prevents interaction with effectors and inactivates RAP2A. Palmitoylated. Palmitoylation is required for association with recycling endosome membranes and activation of TNIK. (Microbial infection) Glucosylated at Thr-35 by C.difficile toxin TcdA in the colonic epithelium, and by P.sordellii toxin TcsL in the vascular endothelium. |
| Function | Small GTP-binding protein which cycles between a GDP-bound inactive and a GTP-bound active form. In its active form interacts with and regulates several effectors including MAP4K4, MINK1 and TNIK. Part of a signaling complex composed of NEDD4, RAP2A and TNIK which regulates neuronal dendrite extension and arborization during development. More generally, it is part of several signaling cascades and may regulate cytoskeletal rearrangements, cell migration, cell adhesion and cell spreading. |
| Protein Name | Ras-Related Protein Rap-2aRbbp-30 |
| Cellular Localisation | Recycling Endosome MembraneLipid-AnchorCytoplasmic SideMidbodyMay Also Localize To The Golgi And The Gelatinase-Containing Granules Of NeutrophilsColocalizes With Rasgef1b To Midbody At Telophase |
| Alternative Antibody Names | Anti-Ras-Related Protein Rap-2a antibodyAnti-Rbbp-30 antibodyAnti-RAP2A antibody |
Information sourced from Uniprot.org
12 months for antibodies. 6 months for ELISA Kits. Please see website T&Cs for further guidance
