| Host: |
Rabbit |
| Applications: |
WB |
| Reactivity: |
Human/Mouse |
| Note: |
STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS. |
| Short Description: |
Rabbit polyclonal antibody anti-UBE2M (1-183) is suitable for use in Western Blot research applications. |
| Clonality: |
Polyclonal |
| Conjugation: |
Unconjugated |
| Isotype: |
IgG |
| Formulation: |
PBS with 0.02% Sodium Azide, 50% Glycerol, pH7.3. |
| Purification: |
Affinity purification |
| Dilution Range: |
WB 1:200-1:2000 |
| Storage Instruction: |
Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles. |
| Gene Symbol: |
UBE2M |
| Gene ID: |
9040 |
| Uniprot ID: |
UBC12_HUMAN |
| Immunogen Region: |
1-183 |
| Immunogen: |
Recombinant fusion protein containing a sequence corresponding to amino acids 1-183 of human UBE2M (NP_003960.1). |
| Immunogen Sequence: |
MIKLFSLKQQKKEEESAGGT KGSSKKASAAQLRIQKDINE LNLPKTCDISFSDPDDLLNF KLVICPDEGFYKSGKFVFSF KVGQGYPHDPPKVKCETMVY HPNIDLEGNVCLNILREDWK PVLTINSIIYGLQYLFLEPN PEDPLNKEAAEVLQNNRRLF EQNVQRSMRGGYIGSTYFER CLK |
| Post Translational Modifications | The acetylation of Met-1 increases affinity for DCUN1D1 by about 2 orders of magnitude and is crucial for NEDD8 transfer to cullins. |
| Function | Accepts the ubiquitin-like protein NEDD8 from the UBA3-NAE1 E1 complex and catalyzes its covalent attachment to other proteins. The specific interaction with the E3 ubiquitin ligase RBX1, but not RBX2, suggests that the RBX1-UBE2M complex neddylates specific target proteins, such as CUL1, CUL2, CUL3 and CUL4. Involved in cell proliferation. |
| Protein Name | Nedd8-Conjugating Enzyme Ubc12Nedd8 Carrier ProteinUbiquitin-Conjugating Enzyme E2 M |
| Database Links | Reactome: R-HSA-2173789Reactome: R-HSA-5607761Reactome: R-HSA-5676590Reactome: R-HSA-8951664Reactome: R-HSA-983168 |
| Alternative Antibody Names | Anti-Nedd8-Conjugating Enzyme Ubc12 antibodyAnti-Nedd8 Carrier Protein antibodyAnti-Ubiquitin-Conjugating Enzyme E2 M antibodyAnti-UBE2M antibodyAnti-UBC12 antibody |
Information sourced from Uniprot.org
12 months for antibodies. 6 months for ELISA Kits. Please see website T&Cs for further guidance
