• Western blot analysis of the lysates from HepG2 cells using RRAGA/B antibody.
  • Western blot analysis of lysates from HepG2 cells, using RRAGA/B Antibody. The lane on the right is blocked with the synthesized peptide.
  • Immunohistochemical analysis of paraffin-embedded Human brain. Antibody was diluted at 1:100 (4°C overnight). High-pressure and temperature Tris-EDTA, pH8.0 was used for antigen retrieval. Negetive contrl (right) obtaned from antibody was pre-absorbed by immunogen peptide.
  • Immunohistochemical analysis of paraffin-embedded Human brain. Antibody was diluted at 1:100 (4°C overnight). High-pressure and temperature Tris-EDTA, pH8.0 was used for antigen retrieval. Negetive contrl (right) obtaned from antibody was pre-absorbed by immunogen peptide.
  • Western blot analysis of HepG2 cells using Rag A/B Polyclonal Antibody diluted at 1：1000
  • Western blot analysis of various cells using Rag A/B Polyclonal Antibody diluted at 1：1000

Anti-Rag A/B antibody (264-313 aa) (STJ95355)

SKU:
STJ95355

£46.00 - £226.00
Current Stock:
Host: Rabbit
Applications: WB/IHC/IF/ELISA
Reactivity: Human/Mouse/Rat
Note: STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS.
Short Description: Rabbit polyclonal antibody anti-Ras-related GTP-binding protein A and Ras-related GTP-binding protein B (264-313 aa) is suitable for use in Western Blot, Immunohistochemistry, Immunofluorescence and ELISA research applications.
Clonality: Polyclonal
Conjugation: Unconjugated
Isotype: IgG
Formulation: Liquid in PBS containing 50% Glycerol, 0.5% BSA and 0.02% Sodium Azide.
Purification: The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.
Concentration: 1 mg/mL
Dilution Range: WB 1:500-1:2000
IHC 1:100-1:300
ELISA 1:20000
IF 1:50-200
Storage Instruction: Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles.
Gene Symbol: RRAGA
RRAGB
Gene ID: 10670
10325
Uniprot ID: RRAGA_HUMAN
RRAGB_HUMAN
Immunogen Region: 264-313 aa
Specificity: Rag A/B Polyclonal Antibody detects endogenous levels of Rag A/B protein.
Immunogen: The antiserum was produced against synthesized peptide derived from the human RRAGA/B at the amino acid range 264-313
Post Translational Modifications Ubiquitinated. 'Lys-68'-linked polyubiquitination of the GDP-bound inactive form of RRAGA by RNF152 is increased in response to amino acid starvation. Polyubiquitination promotes interaction with the GATOR1 complex. This does not affect RRAGA degradation.
Function Guanine nucleotide-binding protein that plays a crucial role in the cellular response to amino acid availability through regulation of the mTORC1 signaling cascade. Forms heterodimeric Rag complexes with RRAGC or RRAGD and cycles between an inactive GDP-bound and an active GTP-bound form. In its active form participates in the relocalization of mTORC1 to the lysosomes and its subsequent activation by the GTPase RHEB. Involved in the RCC1/Ran-GTPase pathway. May play a direct role in a TNF-alpha signaling pathway leading to induction of cell death. (Microbial infection) May alternatively act as a cellular target for adenovirus E3-14.7K, an inhibitor of TNF-alpha functions, thereby affecting cell death.
Protein Name Ras-Related Gtp-Binding Protein A
Rag A
Raga
Adenovirus E3 14.7 Kda-Interacting Protein 1
Fip-1
Database Links Reactome: R-HSA-1632852
Reactome: R-HSA-165159
Reactome: R-HSA-166208
Reactome: R-HSA-380972
Reactome: R-HSA-5628897
Reactome: R-HSA-8866654
Reactome: R-HSA-8943724
Reactome: R-HSA-9639288
Cellular Localisation Cytoplasm
Nucleus
Lysosome
Predominantly Cytoplasmic
May Shuttle Between The Cytoplasm And Nucleus
Depending On The Bound Nucleotide State
Colocalizes In Vivo With Adenovirus E3-14
7k Mainly To The Cytoplasm Especially Near The Nuclear Membrane And In Discrete Foci On Or Near The Plasma Membrane
Alternative Antibody Names Anti-Ras-Related Gtp-Binding Protein A antibody
Anti-Rag A antibody
Anti-Raga antibody
Anti-Adenovirus E3 14.7 Kda-Interacting Protein 1 antibody
Anti-Fip-1 antibody
Anti-RRAGA antibody

Information sourced from Uniprot.org

12 months for antibodies. 6 months for ELISA Kits. Please see website T&Cs for further guidance