• Western blot analysis of extracts of various cell lines, using PREB antibody (STJ116559) at 1:3000 dilution. Secondary antibody: HRP Goat Anti-rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Enhanced Kit. Exposure time: 90s.

Anti-PREB antibody (13-202) (STJ116559)

SKU:
STJ116559

£169.50 - £449.50
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Host: Rabbit
Applications: WB
Reactivity: Human/Mouse
Note: STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS.
Short Description: Rabbit polyclonal antibody anti-PREB (13-202) is suitable for use in Western Blot research applications.
Clonality: Polyclonal
Conjugation: Unconjugated
Isotype: IgG
Formulation: PBS with 0.01% Thimerosal, 50% Glycerol, pH7.3.
Purification: Affinity purification
Dilution Range: WB 1:500-1:2000
Storage Instruction: Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles.
Gene Symbol: PREB
Gene ID: 10113
Uniprot ID: PREB_HUMAN
Immunogen Region: 13-202
Immunogen: Recombinant fusion protein containing a sequence corresponding to amino acids 13-202 of human PREB (NP_037520.1).
Immunogen Sequence: PFPLYALQVDPSTGLLIAAG GGGAAKTGIKNGVHFLQLEL INGRLSASLLHSHDTETRAT MNLALAGDILAAGQDAHCQL LRFQAHQQQGNKAEKAGSKE QGPRQRKGAAPAEKKCGAET QHEGLELRVENLQAVQTDFS SDPLQKVVCFNHDNTLLATG GTDGYVRVWKVPSLEKVLEF KAHEGEIEDL
Tissue Specificity Ubiquitous.
Function Guanine nucleotide exchange factor that specifically activates the small GTPase SAR1B. Mediates the recruitment of SAR1B and other COPII coat components to endoplasmic reticulum membranes and is therefore required for the formation of COPII transport vesicles from the ER. Was first identified based on its probable role in the regulation of pituitary gene transcription. Binds to the prolactin gene (PRL) promoter and seems to activate transcription.
Protein Name Prolactin Regulatory Element-Binding Protein
Mammalian Guanine Nucleotide Exchange Factor Msec12
Database Links Reactome: R-HSA-204005
Reactome: R-HSA-381038
Reactome: R-HSA-5694530
Cellular Localisation Endoplasmic Reticulum Membrane
Single-Pass Membrane Protein
Nucleus
Concentrates At Endoplasmic Reticulum Exit Sites (Eres)
Also Known As Transitional Endoplasmic Reticulum (Ter)
Alternative Antibody Names Anti-Prolactin Regulatory Element-Binding Protein antibody
Anti-Mammalian Guanine Nucleotide Exchange Factor Msec12 antibody
Anti-PREB antibody
Anti-SEC12 antibody

Information sourced from Uniprot.org

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